Genetic diversity and clonal evolution of carbapenem-resistant Acinetobacter baumannii isolates from Portugal and the dissemination of ST118

Manageiro, Vera; Jones-Dias, Daniela; Ferreira, Eugénia; Louro, Deolinda; Caniça, Manuela

doi:10.1016/j.ijantimicag.2012.06.013

Cited by 27 publications

(19 citation statements)

References 28 publications

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“…However, results of phenotypic and genotypic tests in several investigations have also consistence with our result. For example, Shoja reported that 55% of the isolates were positive by DDS test but did not find any MBL producing gene, which is consistent with the studies of Manageiro [26] and Hu [27].…”

Section: Discussionsupporting

confidence: 77%

Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

Khaledi

Abadi

Validi

et al. 2019

Preprint

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Objective: Acinetobacter baumanii is a pathogenic bacterium that is the cause of many nosocomial infections. This study aimed to determine metallo-β-lactamases (MBL) produced by the A . baumanii isolated obtained from clinical samples in Shahrekord, southwest Iran . Results: A total of 100 A . baumanii isolates were isolated from 250 clinical samples between June 2013 and June 2014. Then, the isolates were identified by biochemical tests and MBL screening conducted by the phenotypic tests modified Hodge, EDTA-disk synergy (EDS), combined disk (CD) and AmpC disc after antibiotic sensitivity test, and the bla genes detected by PCR. Eighty-five (85%) isolates were resistant to meropenem and imipenem. Phenotypic tests showed that out of the 100 isolates, 46, 59, 50, 65 and 65 isolates were positive: AmpC disk, CD, EDS, Modified Hodge and Etest MBL, suggesting MBL production, respectively. Gene detection by PCR showed that 23 isolates carried the VIM-1 gene and only three isolates carried the IMP-1 gene. The prevalence of metallo-β-lactamases-containing A . baumanii isolates is increasing, and the coexistence of various carbapenemases is a problem. Continuous monitoring of the infections due to these bacteria should be taken into account in planning for alternative and new therapeutic strategies.

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Section: Discussionsupporting

confidence: 77%

Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

Khaledi

Abadi

Validi

et al. 2019

Preprint

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“…PCR and DNA sequencing were used to screen and identify genes encoding for class A ( bla TEM , bla SHV , bla OXA‐G1 , bla CTX‐M , bla KPC , bla GES ), class B ( bla IMP , bla VIM , bla NDM , imiS, L1 ), class C ( bla CMY , bla ACC , bla ACT, bla DHA, bla MIR, bla FOX ), and class D ( bla OXA‐23 , bla OXA‐24 , bla OXA‐48 , bla OXA‐51 , bla OXA‐58 ) β‐lactamases, using primers specifically designed for this study (Table ), and others previously described (Pérez‐Pérez & Hanson, ; Mendonça et al ., , ; Manageiro et al ., ). PMQR‐encoding genes [ qnrA (Wang et al ., ), qnrB (Wu et al ., ), qnrC (Wang et al ., ), qnrD (Cavaco et al ., ), qnrS (Robicsek et al ., ), qepA (Jones‐Dias et al ., ), and aac(6')Ib‐cr (Park et al ., )] and the presence of class 1 (Leverstein‐Van Hall et al ., ) and class 2 integrase (Jones‐Dias et al ., ) were also investigated by PCR, using specific primers.…”

Section: Methodsmentioning

confidence: 97%

GES-5 among theβ-lactamases detected in ubiquitous bacteria isolated from aquatic environment samples

Manageiro

Ferreira

Caniça

et al. 2013

FEMS Microbiol Lett

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In this study, we investigated the β-lactamase-encoding genes responsible for β-lactam resistance phenotypes detected among 56 Gram-negative isolates (Gamma- and Alpha-proteobacteria) recovered from wastewater, urban streams, and drinking water. The β-lactam resistance mechanisms detected in 36 isolates comprised the presence of class A (bla , bla , bla , bla ), class B (ImiS, L1), class C (bla , bla , bla , bla , bla , bla , bla ), and class D (blaOXA-309)β-lactamase-encoding genes, some variants described for the first time here. Notably, the results showed antimicrobial resistance genes related not only to commonly used antibiotics, but also to carbapenems, providing the first description of a GES-5-producing Enterobacteriaceae. The importance of ubiquitous bacteria thriving in aquatic environments as reservoirs or carriers of clinically relevant resistance determinants was confirmed, and the need to monitor water habitats as potential sources for the emergence and/or spread of antibiotic resistance in the environment was highlighted.

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“…The first description of OXA-23 -producing A. baumannii was reported in Scotland in 1985 (138) . Since the last decade this enzyme has been detected in several countries such as Tunisia (78) , the United Arab Emirates (99) , Bulgaria (143) , Afghanistan and Iraq (16) , Turkey (48) ,Thailand (104) , South Korea (54,151) , Italy (3) ,France (37) , China (31,55) , Portugal (77) , Poland (106) , Greece (69) and Brazil (4,5,18,19,24,84,85,97) .…”

Section: Acinetobacter Baumannii and Production Of Oxa-23-type Carbapmentioning

confidence: 99%

Oxacillinase (OXA)-producing Acinetobacter baumannii in Brazil: clinical and environmental impact and therapeutic options

Medeiros

Lincopán²

2013

J. Bras. Patol. Med. Lab.

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Genetic diversity and clonal evolution of carbapenem-resistant Acinetobacter baumannii isolates from Portugal and the dissemination of ST118

Cited by 27 publications

References 28 publications

Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

Phenotypic and genotypic detection of metallo-β-lactamases in A. baumanii isolates obtained from clinical samples in Shahrekord, southwest Iran

GES-5 among theβ-lactamases detected in ubiquitous bacteria isolated from aquatic environment samples

Oxacillinase (OXA)-producing Acinetobacter baumannii in Brazil: clinical and environmental impact and therapeutic options

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