2019
DOI: 10.21475/ajcs.19.13.07.p1638
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Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers

Abstract: In this study, we aimed to investigate the genetic diversity and polymorphism among 30 soybean genotypes maintained by the ARC using simple sequence repeat (SSR) markers. Soybean genotypes were characterized using 20 SSR primers. DNA was extracted using the standard cetyl trimethylammonium bromide method and amplified using PCR. Allele size was determined via comparison with a 100 base pair (bp) DNA ladder. Molecular data were analyzed, and a dendrogram and matrix were generated using GGT 2.0 software. A total… Show more

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Cited by 11 publications
(10 citation statements)
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“…Genetic Diversity Studies of Soybean [Glycine max (L.) Merrill] Germplasm Accessions using Cluster and Principal Component Analysis extracted using the Principal Component Analysis approach by using the GRAPES software (Kujane et al 2019).…”
Section: Methodsmentioning
confidence: 99%
“…Genetic Diversity Studies of Soybean [Glycine max (L.) Merrill] Germplasm Accessions using Cluster and Principal Component Analysis extracted using the Principal Component Analysis approach by using the GRAPES software (Kujane et al 2019).…”
Section: Methodsmentioning
confidence: 99%
“…The colour of the seed, plant height, seed colour pattern, seed shape and 100 seed weight were recorded at harvest. Using qualitative characters, hierarchical clustering was performed by calculating Euclidean distance and the cluster diagram was constructed by NJ (Neighbour Joining) dendogram using GGT 2.0 Software (Kujane et al, 2019).…”
Section: Methodsmentioning
confidence: 99%
“…The genotypes were scored for each trait based on the note values of the DUS characters given by PPV & FR, 2001. The morphological data were subjected to cluster analysis using GGT 2.0 Software (Kujane et al, 2019). The dendrogram was constructed using UPGMA tree clustering.…”
Section: Dusmentioning
confidence: 99%