Genetic diversity among Turkish local grape accessions (Vitis vinifera L.) using RAPD markers

Karataş, Hüseyin; Ağaoğlu, Y. Sabit

doi:10.1111/j.0018-0661.2008.02011.x

Cited by 22 publications

(6 citation statements)

References 8 publications

(6 reference statements)

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“…In this study, the general similarity ratios among the cultivars were found to be between 0.650 and 0.900, showing a parallelism with the other RAPD analyses performed by other researchers, 0.553-0.952 (Ergül et al, 2002;Karataş, 2005;Ağaoğlu et al, 2006;Karataş and Ağaoğlu, 2008).…”

Section: Resultssupporting

confidence: 89%

RAPD analysis of selected local Turkish grape cultivars (Vitis vinifera)

Karataş

Ys²

2010

Genet. Mol. Res.

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ABSTRACT. Turkey is very rich in local grape varieties. The solution to the problem of identifying local cultivars, which is considered an important deficiency for the region, will only be possible when they can be defined with molecular markers. Forty-nine local grapevine cultivars from Şanlıurfa (Turkey) were characterized with RAPD markers. Twenty-five decamer primers selected from 60 primers were used in this analysis. A total of 171 bands were obtained with the 25 primers, of which 112 were polymorphic; the level of DNA polymorphism was 65.49% in these local cultivars. Among the selected primers, OPA-18, OPO-07 and P-123 gave the maximum number of polymorphic bands (seven). Genetic relationships among these cultivars were determined with a similarity index and using a dendogram. Among the grape cultivars, the lowest similarity ratio (0.578) was observed among the Külahi-Kızılbanki cultivars and the highest similarity ratio (0.908) was observed for the Çilorut-Dökülgen combination. The high similarity ratio among the grape cultivars of Şanlıurfa Province was also reflected in the dendogram. 1981©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 1980-1986 (2010) RAPD analysis of Turkish grape cultivars (Vitis vinifera) In general, no relationships were encountered between the genetic identification of the cultivars and their ampelographic properties.

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Section: Resultssupporting

confidence: 89%

RAPD analysis of selected local Turkish grape cultivars (Vitis vinifera)

Karataş

Ys²

2010

Genet. Mol. Res.

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“…Outside, for some non comprehensive reasons, the genetic variability between accessions of Calligonum is due to the occupied different specific geographical places, and therefore to their capacities to adjust to the specific climatic conditions. The survey of germplasms of the Turk grape suggested that the geographical and ecological distribution of plants contributes to a high genetic variability (Karatas and Agaoglu, 2008). According to Liu and Pei, (1999) and Runo et al (2004), the similar strips gotten by RAPD from different individuals are not necessarily homologous, although, they can share the same dimension in pairs of basis.…”

Section: Discussionmentioning

confidence: 99%

Natural genetic variation in Calligonum Tunisian genus analyzed by RAPD markers

Dhief¹,

Guasmi²,

Triki³

et al. 2013

Afr. J. Biotechnol.

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The Calligonum genus is one of the most economically important resources of the Tunisian desert, playing an important role in the lives of desert local population. A great range of genetic diversity could be seen in diverse populations of this genus which are spread all over Tunisian areas. DNA-based molecular markers are playing increasingly important role in the analysis of genetic diversity in wide range of plants. This study is an attempt to collect, compile and collate information on the existing natural genetic diversity, at intra-specific and inter-populational levels in Calligonum genus (C.azel, C.arich and C.comosum). This study deals with 16 genotypes of Calligonum (Polygonaceae) collected from different regions of Tunisian desert using random amplified polymorphic DNA (RAPD) marker. In total, 46 polymorphic bands amplified by 4 random primers, with the polymorphic rate of 89.06% were recorded. The commercial software package SPSS 16 was used to develop similar matrices based on the Dice coefficient which is defined as 2a/2a+u, where "a" is the number of positive matches and "u" is the number of non-matches. From the analysis, it is imperative that predominantly obligate out breeding behavior of Calligonum genus, helps these diverse accessions to spread and occupy specific geographical niches in the Tunisian arid regions.

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“…For these reasons many fruit tree crops have been successfully fingerprinted using RAPD markers, e.g. grape (Huseyin and Sabitagaoglu, 2008), strawberry (Sugimoto et al, 2005), olive (Sanz-Cortes et al, 2001) and pineapple (Sripaoraya et al, 2001). However, despite its limitations such as sensitivity to reaction conditions, problems with repeatability, and amplification of non-homologous sequences, it has been successfully used for the assessment of genetic diversity in plants (Maria et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

Munankarmi

Shrestha²,

Rana

et al. 2014

Int J Appl Sci Biotechnol

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Acid lime (Citrus aurantifolia Swingle) is an important commercial fruit crop, cultivated from terai to high hill landscapes of Nepal. However, production and productivity is very low due to various reasons including infestations by various diseases and pests, lack of diseases and pests resistant and high yielding varieties. In this context, determination of genetic variation at molecular level is fundamental to citrus breeders for the development of elite cultivars with desirable traits. In the present study, Random Amplified Polymorphic DNA (RAPD) marker technique has been employed to assess genetic diversity in 60 acid lime landraces representing different agro-ecological zones of eastern Nepal. Nine selected arbitrary primers generated 79 RAPD fragments of which 75 were polymorphic (94.94%). Phenogram was constructed by NTSYS-PC ver. 2.21i using UPGMA cluster analysis based on Jaccard's similarity coefficient to deduce overall genetic diversity and relationships of the acidlime genotypes under study. Sixty acid lime landraces formed seven clusters and similarity value ranged from 38% to 98% with an average of 72%. Genetic variation at different agro-ecological zones was assessed using Popgene ver. 1.32 and found 47% to 69.6% polymorphism. Shannon's index and Nei's gene diversity showed highest level of acid lime diversity in Terai zone (PPB, 69.62%; H, 0.213; I, 0.325) followed by mid-hill zone (PPB, 67.09%; H, 0.208; I, 0.317). The results obtained will be useful to citrus breeders for elite cultivar development. The RAPD-PCR technique is found to be the rapid and effective tool for genetic diversity assessment in acid lime landraces of Nepal.

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Genetic diversity among Turkish local grape accessions (Vitis vinifera L.) using RAPD markers

Cited by 22 publications

References 8 publications

RAPD analysis of selected local Turkish grape cultivars (Vitis vinifera)

RAPD analysis of selected local Turkish grape cultivars (Vitis vinifera)

Natural genetic variation in Calligonum Tunisian genus analyzed by RAPD markers

Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

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