Genetic diversity among red swamp crayfish (Procambarus clarkii) populations in the middle and lower reaches of the Yangtze River based on AFLP markers

Zhu, Bi‐Ru; Huang, Yu‐Ching; Dai, Yufei; Bi, Chunxia; Hu, C Y

doi:10.4238/2013.march.13.8

Cited by 14 publications

(8 citation statements)

References 37 publications

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“…This historical report perfectly matches the genetic pattern (i.e. founder effect and strong bottleneck) found in Japanese and, overall, Chinese populations of P. clarkii (Yue et al., ; Li et al., ; Zhu, Huang, Dai, Bi, & Hu, , this study), in which a smaller batch was introduced to Japan and subsequent invasions came from the Japan population with few founders (Figure ). The lack of ectoparasites of the order Branchiobdellida is often attributed to long shipments in poor conditions (Clavero, Nores, Kubersky‐Piredda, & Centeno‐Cuadros, ; Gelder & Williams, ).…”

Section: Discussionsupporting

confidence: 87%

Unravelling the global invasion routes of a worldwide invader, the red swamp crayfish (Procambarus clarkii)

et al. 2019

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Understanding how introduced species succeed and become widely distributed within non‐native areas is critical to reduce the threats posed by them. Our goal was to reconstruct the main invasion routes and invasion dynamics of a global freshwater invader, the red swamp crayfish, Procambarus clarkii, through the analysis of its genetic variability in both native and invasive ranges. We inferred invasion routes and population structure from the analysis of a fragment (608 base pairs) of the mitochondrial marker cytochrome c oxidase subunit I from 1,062 individuals of P. clarkii in addition to 354 GenBank sequences, for a total of 122 populations (22 natives and 100 invaded). Genetic structure was assessed using analysis of molecular variance and non‐metric multidimensional scaling analyses. We analysed haplotype frequencies for the genetic variability in each locality and region. The haplotype network was depicted by using PopART software. A high haplotype diversity was found in the native range (haplotype diversity [Hd]: 0.90), but also in some non‐native areas, such as western U.S.A. (Hd: 0.80), areas of Mexico (Hd: 0.78), and some hotspots in Europe (e.g. southern Spain or Italy), suggesting a complex pattern of multiple introductions. We grouped all localities in five differentiated groups according to biogeographic origin: the native area, west Americas, east U.S.A., Asia, and Europe. Additionally, the identification of 15 haplotypes shared between at least two localities, the phylogenetic network estimation and indices of genetic differentiation among localities allowed us to identify a large genetic admixture in the native range; the two independent invasion routes (i.e. westwards and eastwards) in U.S.A. from the native range (Louisiana and Texas) with translocations within each area; a stepping‐stone introduction from U.S.A. to Japan (involving few individuals) themselves introduced to China afterwards; the entry of P. clarkii from Louisiana (U.S.A.) into southern Spain and their multiple secondary introductions over Europe as well as other possible introductions in central Europe. Our study emphasises the need for unravelling the global invasion routes and the demographic processes underlying the introduction of exotic species (i.e. admixture, bridgehead invasion effect, and propagule pressure) to control the spread of invasive species. Our findings highlight the value of genetic analyses to identify the geographic origin of source populations as well as the variability of invaded areas in order to reconstruct invasion dynamics and facilitate management of invasive species (e.g. through environmental DNA monitoring).

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Section: Discussionsupporting

confidence: 87%

Unravelling the global invasion routes of a worldwide invader, the red swamp crayfish (Procambarus clarkii)

et al. 2019

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“…P. clarkii was first introduced into Nanjing, China, from Japan in 1929 [6], and at present it can be found in freshwater habitats such as rivers, swamps, sloughs, and paddy fields [5]. Although P. clarkii could lead to economic losses and declines biodiversity [7], the crayfish is one of the most important aquaculture resources [7][8][9], since it is welcomed by a vast number of consumers for its delicious taste and high meat quality. As a successful invasive species, P. clarkii has advantageous traits including a short life cycle, high fecundity, and high disease resistance [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…The species is highly adaptable and can disperse widely in the habitat and tolerate diverse environmental conditions [4,10]. Furthermore, P. clarkii has retained high levels of genetic diversity in both wild populations [5,6,9,11,12] and commercial populations [13]; this contributes to avoiding the harmful effects of inbreeding, for adapting to different environments [14], and in the selection of good breeding germplasm for crayfish artificial culture [5]. At present, P. clarkii farming has become an important industry in China, with production reaching 1,638,700 tons and a total output value of 369 billion China Yuan (CNY) in 2018 [15].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii

et al. 2021

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Background The red swamp crayfish Procambarus clarkii is a freshwater species that possesses high adaptability, environmental tolerance, and fecundity. P. clarkii is artificially farmed on a large scale in China. However, the molecular mechanisms of ovarian development in P. clarkii remain largely unknown. In this study, we identified four stages of P. clarkii ovary development, the previtellogenic stage (stage I), early vitellogenic stage (stage II), middle vitellogenic stage (stage III), and mature stage (stage IV) and compared the transcriptomics among these four stages through next-generation sequencing (NGS). Results The total numbers of clean reads of the four stages ranged from 42,013,648 to 62,220,956. A total of 216,444 unigenes were obtained, and the GC content of most unigenes was slightly less than the AT content. Principal Component Analysis (PCA) and Anosim analysis demonstrated that the grouping of these four stages was feasible, and each stage could be distinguished from the others. In the expression pattern analysis, 2301 genes were continuously increase from stage I to stage IV, and 2660 genes were sharply decrease at stage IV compared to stages I-III. By comparing each of the stages at the same time, four clusters of differentially expressed genes (DEGs) were found to be uniquely highly expressed in stage I (136 genes), stage II (43 genes), stage III-IV (49 genes), and stage IV (22 genes), thus exhibiting developmental stage specificity. Moreover, in comparisons between adjacent stages, the number of DEGs between stage III and IV was the highest. GO enrichment analysis demonstrated that nutrient reservoir activity was highest at stage II and that this played a foreshadowing role in ovarian development, and the GO terms of cell, intracellular and organelle participated in the ovary maturation during later stages. In addition, KEGG pathway analysis revealed that the early development of the ovary was mainly associated with the PI3K-Akt signaling pathway and focal adhesion; the middle developmental period was related to apoptosis, lysine biosynthesis, and the NF-kappa B signaling pathway; the late developmental period was involved with the cell cycle and the p53 signaling pathway. Conclusion These transcriptomic data provide insights into the molecular mechanisms of ovarian development in P. clarkii. The results will be helpful for improving the reproduction and development of this aquatic species.

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“…Undoubtedly, P. clarkii could provide a desirable mode for investigating the genetic structure of an invasive species mainly dispersed by human-mediated factors. Previous studies 6 , 12 , 13 proposed that the populations of P. clarkii in China exhibited a high level of genetic diversity after successfully invading a new environment. Meanwhile, in the past years, the human-mediated dispersal in aquaculture accelerated the invasion of P. clarkii in China and might result in the differnet levels of gene flow among different populations 11 .…”

Section: Introductionmentioning

confidence: 99%

“…The population structure and genetic diversity of this alien species help us revealing the historical dispersal pattern of P. clarkii in China, and also provide new insights into invasion prevention and utilization of genetic resource. Previously, population genetic studies of P. clarkii have generally used mitochondrial genes 12 , microsatellites 14 , 15 and AFLP 13 , and that the genomic data of P. clarkii is quite limited 8 , 16 . The growing accessibility to high-throughput sequencing technologies allows the production of massive data and the discovery of genome-wide resources at relatively modest and decreasing costs.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Population Genetic Structure of red swamp crayfish, Procambarus clarkii, in China

Shi

et al. 2018

Sci Rep

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The red swamp crayfish (Procambarus clarkii) is one of the most economically important farmed aquatic species in China. However, it is also a famous invasive species in the world. This invasive species was dispersed most via human activities including intentional or unintentional carry in China. Thus, P. clarkii naturally distributed in China provides us a desirable mode to investigate the genetic structure of an invasive species dispersed mainly by human-mediated factors. To reveal the impact of human-mediated dispersal on genetic structure of P. clarkii in China, a total of 22,043 genome-wide SNPs were obtained from approximately 7.4 billion raw reads using 2b-RAD technique in this study. An evident pattern of population genetic structure and the asymmetrical migrational rates between different regions were observed with 22 populations based on these SNPs. This study provide a better understanding of the population genetic structure and demographic history of P. clarkii populations in China, inferring that anthropogenic factors (aquaculture or by accident) and ecological factors (e.g., complicated topography and climatic environment), as well as its special biological traits could account for the current population structure pattern and dispersal history of P. clarkii.

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Genetic diversity among red swamp crayfish (Procambarus clarkii) populations in the middle and lower reaches of the Yangtze River based on AFLP markers

Cited by 14 publications

References 37 publications

Unravelling the global invasion routes of a worldwide invader, the red swamp crayfish (Procambarus clarkii)

Unravelling the global invasion routes of a worldwide invader, the red swamp crayfish (Procambarus clarkii)

Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii

Characterization of Population Genetic Structure of red swamp crayfish, Procambarus clarkii, in China

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