Genetic differentiation between Cinta Senese and commercial pig breeds using microsatellite

Abstract

Background: Cinta Senese (CS) is an autochthonous Tuscan breed, which risked extinction since the '60s. Results: Monitoring the genetic variability of the actual population by use of DNA molecular markers is essential to address a correct breeding policy, finalized to obtain the race preservation and its fitness in the future. 17 SSRs autosomal markers and 1 associated to the X chromosome were used to genotype 86 individuals belonging to the CS and 12 belonging to two main white races Landrace (L), Large White (LW) and crosses between LW and L and L and CS widespread in Tuscany and used in the recent past to obtain hybrids with the CS. Conclusions: A dendrogram of similarity measures the relative genetic distance between individuals in the population. Data show that CS pigs have a distinct genotype from L, LW, LW x L and L x CS.

Genetic Traceability of Cinta Senese Pig (<i>Sus scrofa domesticus</i> L.): A Study of the Meat and Processed Products by Microsatellite Markers

“…The genomic DNA of all samples (meats, salami and hams) were amplified at 8 SSRs loci (S0026, SW240, S0068, SW122, SW632, SW1828, SW1067, S0226) ( Table 1) recommended by ISAG/FAO and selected as most suitable for genetic identification of CS breed according to polymorphic information and allele frequency reported by [53].…”

“…The meats coming from six different CS pigs post-mortem were genotyped at 8 SSRs loci (S0026, SW240, S0068, SW122, SW632, SW1828, SW1067, S0226) [53]. The allele sizes characterizing the genotype of each CS pig post-mortem were used for the production of a dendrogram of similarity by NTSYS 2.0, including the different pig breeds belonging to our private databank listed in Table 2 [53].…”

“…The allele sizes characterizing the genotype of each CS pig post-mortem were used for the production of a dendrogram of similarity by NTSYS 2.0, including the different pig breeds belonging to our private databank listed in Table 2 [53].…”

“…The most commonly used molecular markers for genetic identification and traceability in forensic and animal/crop sciences are the SSRs or microsatellite [51]- [53]. These molecular markers are highly polymorphic, informative and interspersed throughout the entire genome and for their characteristics have been proposed for tracking food, including meat and wine [54].…”

“…Recent works showed as SSR markers are suitable for distinguish genotypically the Cinta Senese breed from the main white breeds spread in Italy and that the use of a general, neutral SSRs genotyping approach, seems appropriate in determining the pig breed identity [53]. Therefore, the aim of this work was to verify the utility of the SSR markers for the implementation of a molecular traceability system through the whole Cinta Senese production chain, from meat to processed products.…”