Genetic detection of hantaviruses in rodents, Albania

Papa, Anna; Rogozi, Elton; Velo, Enkelejda; Papadimitriou, Εvangelia; Bino, Silvia

doi:10.1002/jmv.24486

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Phylogenetic analysis revealed that two variants of DOBV circulate in Romania . In Albania, 263 small mammals were trapped during 2006‐2007; 10 of 148 A. flavicollis rodents were found to be infected with DOBV …”

Section: Discussionmentioning

confidence: 99%

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Dobrava hantavirus variants found in Apodemus flavicollis mice in Kırklareli Province, Turkey

Polat

Sironen²,

Plyusnina³

et al. 2018

Journal of Medical Virology

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Hantaviruses infect humans via inhalation of viral particles within secretions of infected rodents or rarely through direct contact with infected rodents. Determining the prevalence of hantavirus infections among rodent populations is of vital importance to obtain information on hantavirus-related cases and to predict possible outbreaks. We hypothesized that DOBV strains circulating in the Thrace Region in Turkey would be related to other Balkan DOBV strains. In this study, hantavirus infections in the rodent population of the Kırklareli-İğneada Region (north-western Turkey, near the Bulgarian border) were investigated. This region is of particular importance, as it is located in the south-eastern margin of the European continent and was used as an entrance point of Asian faunal elements into Europe. DOBV infection was detected in eight of 73 rodents; all were of the Apodemus flavicollis species. Partial sequences of the viral S-, M-, and L-genome segments were recovered and compared with previously reported DOBV sequences. The newly characterized Turkish strains were similar to other DOBV variants. Silent nucleotide mutations were dominant. The hantavirus prevalence in the İğneada region was similar to what has been reported in Greece and Bulgaria. For the first time, the M-segment sequences of DOBV from Turkey were recovered and genetic data of hantaviruses from Thrace region of Turkey were obtained.

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Section: Discussionmentioning

confidence: 99%

“…Prevention is important due to the lack of effective antivirals . Surveillance offers important data on the infection in wildlife and hantavirus prevalence and thus facilitates prediction of possible outbreaks …”

Section: Introductionmentioning

confidence: 99%

Dobrava hantavirus variants found in Apodemus flavicollis mice in Kırklareli Province, Turkey

Polat

Sironen²,

Plyusnina³

et al. 2018

Journal of Medical Virology

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“…Their prevalence in Western Palearctic and common status in Western and Central Europe made them one of the model organisms to study post-glacial movement of mammals [24, 45]. Both species have traditionally been studied in a parasitological context, as one of the vectors of Borellia -carrying ticks Ixodes ricinus , who often feed on Apodemus [13, 61], tick-borne encephalitis virus [47] and hantaviruses [33, 50] and have been used as markers for environmental quality [40, 66]. Lastly, they have extra-autosomal chromosomes, called B chromosomes, with varied distribution among the populations [59] but unknown role, although it has been suggested they are involved in cellular metabolism [34, 39].…”

Section: Introductionmentioning

confidence: 99%

Population structure of Apodemus flavicollis and comparison to Apodemus sylvaticus in northern Poland based on RAD-seq

Cerezo

Kučka

Zub

et al. 2019

Preprint

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Background: Mice of the genus Apodemus are one the most common mammals in the Palaearctic region. Despite their broad range and long history of ecological observations, there are no whole-genome data available for Apodemus, hindering our ability to further exploit the genus in evolutionary and ecological genomics context.Results: Here we present results from the whole-genome, high-density genotyping using double-digest restriction site-associated DNA sequencing (ddRAD-seq) on 72 individuals of A. flavicollis and 10 A. sylvaticus from four populations, sampled across 500 km distance in northern Poland. Our data present clear genetic divergence of the two species, with average sequence divergence, based on 21377 common loci, of 1.51% and an evolutionary rate of 0.0019 substitutions per site per million years. We provide a catalogue of 117 highly divergent loci that enable genetic differentiation of the two species in Poland and to a large degree of 20 unrelated samples from several European countries and Tunisia. We also show evidence of admixture between the three A. flavicollis populations but demonstrate that they have negligible average genome-wide population structure, with largest pairwise F ST < 0.086. Conclusion:Our study demonstrates the feasibility of genome-wide, high-density genotyping in Apodemus and provides the first insights into the population genomics of one of the species. 1 2 Background 3 Mice of the genus Apodemus (Kaup, 1829) (Rodentia: Muridae) are one the most 4 common mammals in the Palaearctic region [43]. The genus comprises of three 5 subgenera (Sylvaemus, Apodemus and Karstomys) [43], however the systematic 6 classification of the 20 species belonging to the genus [16] is not fully settled [36]. 7 In the Western Palearctic, the yellow-necked mice A. flavicollis (Melchior, 1934) 8 and the woodmice A. sylvaticus (Linnaeus, 1758) are widespread, sympatric and 9 occasionally syntopic species. They are often difficult to distinguish morphologically 10 in their southern range [30], but in the Central and Northern Europe both are 11 easily recognisable by the yellow collar around the neck of A. flavicollis, absent in 12 A. sylvaticus. 13 Their prevalence in Western Palearctic and common status in Western and Central 14 Europe made them one of the model organisms to study post-glacial movement of context, as one of the vectors of Borellia-carrying ticks Ixodes ricinus, who often feed 17 on Apodemus [13, 61], tick-borne encephalitis virus [47] and hantaviruses [33, 50] 18 and have been used as markers for environmental quality [40, 66]. Lastly, they 19 have extra-autosomal chromosomes, called B chromosomes, with varied distribution 20 among the populations [59] but unknown role, although it has been suggested they 21are involved in cellular metabolism [34, 39]. 22Previous studies on Apodemus typically employed a small number of microsatellite 23 [62] and mtDNA markers [24, 42, 44, 45], which are insufficient to learn about the 24 species' population structure and admixture patterns in detail,...

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“…In Albania, the major causative hantavirus agent is Dobrava-Belgrade virus (DOBV). At least three DOBV genotypes have been identified, in association with Apodemus flavicollis, A. agrarius and A. ponticus hosts, DOBV associated with A. flavicollis has been found to be more pathogenic with a fatality rate up to 12% [12][13][14]. According to Papa et al, 2016, DOBV is the only hantavirus detected in Albania and A. flavicollis was the only DOBV-positive rodent species [12,13,15].…”

Section: Discussionmentioning

confidence: 99%

Pancreatitis in patients with hemorrhagic fever with renal syndrome: A five-year experience

Puca¹,

Harxhi²,

Pipero³

et al. 2017

J Infect Dev Ctries

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Introduction: Pancreatitis is a rare complication of hemorrhagic fever with renal syndrome (HFRS). The causative agents of HFRS are hantaviruses, which belong to the genus Hantavirus, family Bunyaviridae. The purpose of this study was to evaluate cases of acute pancreatitis (AP) in patients with HFRS at the Service of Infectious Diseases, Tirana, Albania. Methodology: In this retrospective study, clinical and laboratory data was obtained from 36 patients with confirmed HFRS, between January 2011 and December 2016. The diagnosis had been confirmed by a positive enzyme-linked immunosorbent assay (ELISA) for IgM or IgG antibodies to hantavirus. Results: The average patient age was 39.7 ± 14.1 years with a range of 15-59 years. From 36 HFRS patients, four (11.1%) were found to have AP, all were male. Abdominal pain was the most common symptom and an increase in amylase and lipase was observed in all four patients. Abdominal computed tomography (CT) indicated pancreatitis with surrounding edema, necrosis and hemorrhage. In this study, the total mortality was 11.1% (4/36), while mortality in patients with AP was 25% (1/4). Conclusions: The results indicate that AP is a serious complication of HFRS, with a poor prognosis. Increased awareness of AP in clinicians and assessment of amylasemia or lipasemia in patients with HFRS should be considered, especially in endemic areas where a rapid diagnosis is crucial for a positive outcome.

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Genetic detection of hantaviruses in rodents, Albania

Cited by 7 publications

References 14 publications

Dobrava hantavirus variants found in Apodemus flavicollis mice in Kırklareli Province, Turkey

Dobrava hantavirus variants found in Apodemus flavicollis mice in Kırklareli Province, Turkey

Population structure of Apodemus flavicollis and comparison to Apodemus sylvaticus in northern Poland based on RAD-seq

Pancreatitis in patients with hemorrhagic fever with renal syndrome: A five-year experience

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