Genetic damage following introduction of DNA in Phycomyces

Obraztsova, Irina; Prados, Nicolás; Holzmann, Klaus; Ávalos, Javier; Cerdá‐Olmedo, Enrique

doi:10.1016/j.fgb.2003.09.007

Cited by 33 publications

(18 citation statements)

References 70 publications

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“…They suggested that stable integrative transformation can be achieved in Zygomycetes only through the introduction of endogenous or closely related DNA. In some previous studies, a Zygomycetes defence mechanism was suggested that eliminates exogenous DNA via rearrangements and deletions [22,23,26]. Our results suggest that this mechanism may well be a general characteristic of the Mucoralean fungi, but This study had demonstrated the introduction and expression of the hph and gfp genes into the Backusella genome via ATMT.…”

supporting

confidence: 49%

Agrobacterium tumefaciens ‐mediated transformation of the zygomycete fungus Backusella lamprospora

Nyilasi

Papp

Csernetics

et al. 2008

J. Basic Microbiol.

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The Agrobacterium -mediated transformation was adapted to Backusella lamprospora, a zygomycete fungus closely related to Mucor. The transforming plasmid contained the hygromycin B resistance (hph) and the green fluorescent protein (gfp) genes under the control of the regulator regions of the Mucor circinelloides gpd1 gene. The presence of the hph and gfp genes in the transformants was detected by PCR. The introduced genes could also be amplified directly from the spores of the transformants. The transformation efficiency was investigated by fluorescence microscopy of the transformed spores. A gradual decrease in the hygromycin B resistance was observed during several cultivation cycles: the growth of the transformants on the selection medium became slower, and the detection of the introduced gene became more difficult.

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supporting

confidence: 49%

Agrobacterium tumefaciens ‐mediated transformation of the zygomycete fungus Backusella lamprospora

Nyilasi

Papp

Csernetics

et al. 2008

J. Basic Microbiol.

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“…We suspect that the discovery of adenine methylation being strongly associated with gene expression may explain some of the historic difficulty in genetically modifying early-diverging fungi 18,19 and consequently may be crucial to developing successful transformation techniques within these (and other) challenging eukaryotic systems. For example, taking MAC positioning and features into consideration when designing constructs may be important for achieving methylation and sufficient expression of target genes.…”

mentioning

confidence: 99%

Widespread adenine N6-methylation of active genes in fungi

et al. 2017

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N6-methyldeoxyadenine (6mA) is a noncanonical DNA base modification present at low levels in plant and animal genomes 1-4 , but its prevalence and association with genome function in other eukaryotic lineages remains poorly understood. Here we report that abundant 6mA is associated with transcriptionally active genes in early-diverging fungal lineages 5 . Using single-molecule long-read sequencing of 16 diverse fungal genomes, we observed that up to 2.8% of all adenines were methylated in early-diverging fungi, far exceeding levels observed in other eukaryotes and more derived fungi. 6mA occurred symmetrically at ApT dinucleotides and was concentrated in dense methylated adenine clusters surrounding the transcriptional start sites of expressed genes; its distribution was inversely correlated with that of 5-methylcytosine. Our results show a striking contrast in the genomic distributions of 6mA and 5-methylcytosine and reinforce a distinct role for 6mA as a gene-expressionassociated epigenomic mark in eukaryotes.

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“…The mechanism whereby SexM or SexP, encoded by the sex locus of Phycomyces, contributes to UPI remains to be elucidated. The absence of a stable transformation system for this species prevents gene manipulation approaches to explore this trait (27). Nevertheless, finding this correlation between mating type and UPI in Phycomyces provides research directions in more tractable eukaryotes, to test if the genes required for sex determination also control UPI more widely.…”

Section: Resultsmentioning

confidence: 99%

Sex Determination Directs Uniparental Mitochondrial Inheritance in Phycomyces

Shakya

Idnurm

2014

Eukaryot Cell

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Uniparental inheritance (UPI) of mitochondria is common among eukaryotes. The underlying molecular basis by which the sexes of the parents control this non-Mendelian pattern of inheritance is yet to be fully understood. Two major factors have complicated the understanding of the role of sex-specific genes in the UPI phenomenon: in many cases (i) fusion occurs between cells of unequal size or (ii) mating requires a large region of the genome or chromosome that includes genes unrelated to sex determination. The fungus Phycomyces blakesleeanus is a member of the Mucoromycotina and has a simple mating type locus encoding only one high-mobility group (HMG) domain protein, and mating occurs by fusion of isogamous cells, thus providing a model system without the limitations mentioned above. Analysis of more than 250 progeny from a series of genetic crosses between wild-type strains of Phycomyces revealed a correlation between the individual genes in the mating type locus and UPI of mitochondria. Inheritance is from the plus (؉) sex type and is associated with degradation of the mtDNA from the minus (؊) parent. These findings suggest that UPI can be directly controlled by genes that determine sex identity, independent of cell size or the complexity of the genetic composition of a sex chromosome.

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Genetic damage following introduction of DNA in Phycomyces

Cited by 33 publications

References 70 publications

Agrobacterium tumefaciens ‐mediated transformation of the zygomycete fungus Backusella lamprospora

Agrobacterium tumefaciens ‐mediated transformation of the zygomycete fungus Backusella lamprospora

Widespread adenine N6-methylation of active genes in fungi

Sex Determination Directs Uniparental Mitochondrial Inheritance in Phycomyces

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