Genetic Complementation of the Obligate Marine Actinobacterium Salinispora tropica with the Large Mechanosensitive Channel Gene
            <i>mscL</i>
            Rescues Cells from Osmotic Downshock

Bucarey, Sergio A.; Penn, Kevin; Paul, Lauren A.; Fenical, William; Jensen, Paul R.

doi:10.1128/aem.00577-12

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…Marine adaptation genes (MAGs) were identified from S. tropica and S. arenicola by comparative genomic analysis, and the loss of a mechanosensitive channel gene mscL was thought to result in the inability of Salinispora strains to grow in low osmotic environment. The finding was later confirmed by genetic complementation of S. tropica with mscL ( Bucarey et al, 2012 ). By the genomic comparison of two sponge-derived Streptomyces albus isolates with their terrestrial relative S. albus J1074, several putative MAGs linked to electron transport and potassium uptake were identified ( Ian et al, 2014 ).…”

Section: Introductionmentioning

confidence: 78%

Comparative Genomics Provides Insights Into the Marine Adaptation in Sponge-Derived Kocuriaflava S43

et al. 2018

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Sponge-derived actinomycetes represent a significant component of marine actinomycetes. Members of the genus Kocuria are distributed in various habitats such as soil, rhizosphere, clinical specimens, marine sediments, and sponges, however, to date, little is known about the mechanism of their environmental adaptation. Kocuria flava S43 was isolated from a coastal sponge. Phylogenetic analysis revealed that it was closely related to the terrestrial airborne K. flava HO-9041. In this study, to gain insights into the marine adaptation in K. flava S43 we sequenced the draft genome for K. flava S43 by third generation sequencing (TGS) and compared it with those of K. flava HO-9041 and some other Kocuria relatives. Comparative genomics and phylogenetic analyses revealed that K. flava S43 might adapt to the marine environment mainly by increasing the number of the genes linked to potassium homeostasis, resistance to heavy metals and phosphate metabolism, and acquiring the genes associated with electron transport and the genes encoding ATP-binding cassette (ABC) transporter, aquaporin, and thiol/disulfide interchange protein. Notably, gene acquisition was probably a primary mechanism of environmental adaptation in K. flava S43. Furthermore, this study also indicated that the Kocuria isolates from various marine and hyperosmotic environments possessed common genetic basis for environmental adaptation.

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Section: Introductionmentioning

confidence: 78%

Comparative Genomics Provides Insights Into the Marine Adaptation in Sponge-Derived Kocuriaflava S43

et al. 2018

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“…The majority of their secondary metabolite biosynthetic gene clusters are located in genomic islands, which was used to suggest the products provide ecologically relevant adaptive traits 36 . The genus is unique among the Micromonosporaceae in that all strains tested to date fail to grow when seawater is replaced with deionized water in the growth medium, which was subsequently linked to a variety of marine adaptation genes using both bioinformatic 37 and experimental approaches 38 . However, the primary interest in this taxon has focused on its ability to produce unique and biologically active secondary metabolites.…”

Section: Discovery Of the Genusmentioning

confidence: 99%

The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery

2015

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This review covers the initial discovery of the marine actinomycete genus Salinispora through its development as a model for natural product research. A focus is placed on the novel chemical structures reported with reference to their biological activities and the synthetic and biosynthetic studies they have inspired. The time line of discoveries progresses from more traditional bioassay-guided approaches through the application of genome mining and genetic engineering techniques that target the products of specific biosynthetic gene clusters. This overview exemplifies the extraordinary biosynthetic diversity that can emanate from a narrowly defined genus and supports future efforts to explore marine taxa in the search for novel natural products.

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“…Loss of a particular gene encoding a mechanosensitive channel required for growth in the media with low osmotic strength has been noted for Salinispora . Later, Bucarey et al [5] have demonstrated that complementation of this lost gene restores the capacity of Salinispora to grow on media without sea water. Unfortunately, only a few genome sequences of marine actinomycetes are currently available, thus making studies on marine adaptation of these bacteria rather difficult.…”

Section: Introductionmentioning

confidence: 99%

Genomics of Sponge-Associated Streptomyces spp. Closely Related to Streptomyces albus J1074: Insights into Marine Adaptation and Secondary Metabolite Biosynthesis Potential

et al. 2014

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A total of 74 actinomycete isolates were cultivated from two marine sponges, Geodia barretti and Phakellia ventilabrum collected at the same spot at the bottom of the Trondheim fjord (Norway). Phylogenetic analyses of sponge-associated actinomycetes based on the 16S rRNA gene sequences demonstrated the presence of species belonging to the genera Streptomyces, Nocardiopsis, Rhodococcus, Pseudonocardia and Micromonospora. Most isolates required sea water for growth, suggesting them being adapted to the marine environment. Phylogenetic analysis of Streptomyces spp. revealed two isolates that originated from different sponges and had 99.7% identity in their 16S rRNA gene sequences, indicating that they represent very closely related strains. Sequencing, annotation, and analyses of the genomes of these Streptomyces isolates demonstrated that they are sister organisms closely related to terrestrial Streptomyces albus J1074. Unlike S. albus J1074, the two sponge streptomycetes grew and differentiated faster on the medium containing sea water. Comparative genomics revealed several genes presumably responsible for partial marine adaptation of these isolates. Genome mining targeted to secondary metabolite biosynthesis gene clusters identified several of those, which were not present in S. albus J1074, and likely to have been retained from a common ancestor, or acquired from other actinomycetes. Certain genes and gene clusters were shown to be differentially acquired or lost, supporting the hypothesis of divergent evolution of the two Streptomyces species in different sponge hosts.

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Genetic Complementation of the Obligate Marine Actinobacterium Salinispora tropica with the Large Mechanosensitive Channel Gene mscL Rescues Cells from Osmotic Downshock

Cited by 14 publications

References 28 publications

Comparative Genomics Provides Insights Into the Marine Adaptation in Sponge-Derived Kocuriaflava S43

Comparative Genomics Provides Insights Into the Marine Adaptation in Sponge-Derived Kocuriaflava S43

The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery

Genomics of Sponge-Associated Streptomyces spp. Closely Related to Streptomyces albus J1074: Insights into Marine Adaptation and Secondary Metabolite Biosynthesis Potential

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