Genetic characterization and ligand specificity of the ovine Fc gamma receptor I (ovFcγRІ)

Qiao, Songlin; Liu, Yunchao; Zhang, Jiu‐liang; Yang, Suzhen; Wan, Bo; PingLing, Shi; Zhang, Hong; Guo, Junqing; Zhang, Gaiping

doi:10.1016/j.vetimm.2010.06.002

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…PRRSV has antigenic variability and macrophage affinity, and the main structural proteins encoded by genomes are: GP3, GP4, GP5, M, and N. Current studies indicate that GP5 and N are responsible for the PRRSV ADE (Table 1). It has been shown that GP5 can induce NAbs, but there is high variability in immunedominant non-neutralizing epitope A (aa [27][28][29][30][31][32][33][34][35] in the external structure domain of GP5, which completely covers neutralizing epitope B (aa 37-45), reduces the neutralizing effect, and produces ADE [38]. The N protein of PRRSV is highly conserved, and the antibodies against N are all non-NAbs.…”

Section: Common Characteristics Of the Viruses That Are Able To Induc...mentioning

confidence: 99%

“…FcRs can be divided into Fc gamma receptors (FcγRs), Fc epsilon receptors (FcεR), Fc alpha receptor (FcαR), and Fc delta receptor (FcδR). FcγRs play important roles in Fc-ADE, binding to IgG antibodies and mediating the interactions between immune cells and immune complex [27]. Three types of FcγR are present in humans: FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16).…”

Section: Introductionmentioning

confidence: 99%

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Antibody-Dependent Enhancement: ″Evil″ Antibodies Favorable for Viral Infections

Yang

Zhang

Zhao

et al. 2022

Viruses

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The pandemics caused by emerging viruses such as severe acute respiratory syndrome coronavirus 2 result in severe disruptions to public health. Vaccines and antibody drugs play essential roles in the control and prevention of emerging infectious diseases. However, in contrast with the neutralizing antibodies (NAbs), sub- or non-NAbs may facilitate the virus to enter the cells and enhance viral infection, which is termed antibody-dependent enhancement (ADE). The ADE of most virus infections is mediated by the Fc receptors (FcRs) expressed on the myeloid cells, while others are developed by other mechanisms, such as complement receptor-mediated ADE. In this review, we comprehensively analyzed the characteristics of the viruses inducing FcRs-mediated ADE and the new molecular mechanisms of ADE involved in the virus entry, immune response, and transcription modulation, which will provide insights into viral pathogenicity and the development of safer vaccines and effective antibody drugs against the emerging viruses inducing ADE.

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Section: Common Characteristics Of the Viruses That Are Able To Induc...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibody-Dependent Enhancement: ″Evil″ Antibodies Favorable for Viral Infections

Yang

Zhang

Zhao

et al. 2022

Viruses

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“…A diverse range of Fc receptors (FcRs), widely expressed on the surface of immune cells, can bind specifically to the Fc fragment of antibodies and promote the activation of immune cells and immune complexes to trigger and regulate the immune response after formation of the FcR-Fc complex (Park et al 1984;McCann et al 2010). FcRs can be divided into FccR IgG receptors (including FccRI,FccRIIA,FccRIIB1,FccRIIB2,FccRIIIA and FccRIIIB), FceR IgE receptors (including FceRI and FceRII), FcaR IgA receptors and FcdR IgD receptors based on the different ligands they bind (Qiao et al 2010). Activation of IgE and FceR is the main cause of type I allergic reactions (Suzuki et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

et al. 2018

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Porcine reproductive and respiratory syndrome virus (PRRSV) shows characteristic antibody-dependent enhancement (ADE) of infection and causes porcine systemic inflammation, which is similar to a type I allergic reaction; however, the role of porcine FcεRI in ADE is still unclear. In this study, the expression of different Fc receptors (FcRs) on macrophages was investigated in a PRRSV 3D4/21 cell infection model in the presence or absence of PRRSV antibody. The transcription level of FcγII and FcεRI was significantly up-regulated under PRRSV-antibody complex infection. Internalization and proliferation of PRRSV were promoted by the ADE mechanism when FcεRI was expressed in permissive 3D4/21 cells and the non-permissive cell line HEK 293T. Transcriptome sequencing data showed that the expression levels of AKT, ERK and other signal molecules in the anti-inflammatory pathway were significantly increased, especially in the cells infected with the PRRSV-antibody immune complex. Inflammatory regulatory molecules such as PLA2G6, LOX, TRPM8 and TRPM4 were significantly up-regulated following PRRSV infection but significantly down-regulated in the cells infected with the PRRSV-antibody immune complex. Our results demonstrated that FcεRI could be involved in PRRSV ADE, the antigen presenting process and regulation of the inflammatory response during PRRSV infection, which provides new insights into PRRSV infection mediated by FcεRI and the PRRSV-antibody immune complex.

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Cloning and characterization of ovine immunoglobulin G Fc receptor III (FcγRIII)

Liu

Qiao

Wang

et al. 2011

Veterinary Immunology and Immunopathology

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Receptors for the Fc regions of immunoglobin G (IgG) play a critical role in immunoregulation and immune defenses against pathogens. In this study, we describe the cloning, eukaryotic expression and IgG subclass specificity of ovine Fc gamma receptor III (FcγRIII). The newly cloned ovine FcγRIII cDNA contains a 940 bp open-reading frame (ORF), and is predicted to encode a 250 amino acid transmembrane glycoprotein composed of two immunoglobulin-like extracellular domains, a transmembrane region and a short cytoplasmic tail. The overall identity of the ovine FcγRIII amino acid sequence to its cattle, pig and human counterparts was 83.2%, 62.0%, 60.7%, respectively. Overlapping PCR was performed with the extracellular domain of ovine FcγRIII and the transmembrane and intracellular region of ovine Fc gamma chain to construct a chimeric receptor. Rosetting analysis showed that transfected COS-7 cells required Fc receptor gamma chain for the expression of FcγRIII on the surface. COS-7 cells expressing FcγRIII were able to bind chicken erythrocytes sensitized with ovine IgG1, but not IgG2. Identification of ovine FcγRIII will further our understanding of the ovine immune system.

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Genetic characterization and ligand specificity of the ovine Fc gamma receptor I (ovFcγRІ)

Cited by 4 publications

References 20 publications

Antibody-Dependent Enhancement: ″Evil″ Antibodies Favorable for Viral Infections

Antibody-Dependent Enhancement: ″Evil″ Antibodies Favorable for Viral Infections

Porcine FcεRI Mediates Porcine Reproductive and Respiratory Syndrome Virus Multiplication and Regulates the Inflammatory Reaction

Cloning and characterization of ovine immunoglobulin G Fc receptor III (FcγRIII)

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