GENETIC ANALYSIS OF PROTEIN EXPORT IN <i>ESCHERICHIA COLI</i> K12

Benson, Spencer A.; Hall, Michael N.; Silhavy, Thomas J.

doi:10.1146/annurev.bi.54.070185.000533

Cited by 329 publications

(90 citation statements)

References 83 publications

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“…Our initial work focused on the signal sequence for the Escherichia coli outer membrane protein maltoporin, also called LamB (the kphage receptor), because extensive genetic studies in the Silhavy lab [7][8][9][10] had yielded a number of defective mutants as well as so-called pseudorevertants, in which second site mutations led to restored function. 10 These LamB signal sequences allowed us to correlate conformational behavior with ability to perform the required functions in targeting.…”

Section: Signal Peptide Conformations and Membrane Interactionsmentioning

confidence: 99%

Use of synthetic signal sequences to explore the protein export machinery

Clérico¹,

Maki²,

Gierasch³

2008

Biopolymers

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The information for correct localization of newly synthesized proteins in both prokaryotes and eukaryotes resides in self‐contained, often transportable targeting sequences. Of these, signal sequences specify that a protein should be secreted from a cell or incorporated into the cytoplasmic membrane. A central puzzle is presented by the lack of primary structural homology among signal sequences, although they share common features in their sequences. Synthetic signal peptides have enabled a wide range of studies of how these “zipcodes” for protein secretion are decoded and used to target proteins to the protein machinery that facilitates their translocation across and integration into membranes. We review research on how the information in signal sequences enables their passenger proteins to be correctly and efficiently localized. Synthetic signal peptides have made possible binding and crosslinking studies to explore how selectivity is achieved in recognition by the signal sequence‐binding receptors, signal recognition particle, or SRP, which functions in all organisms, and SecA, which functions in prokaryotes and some organelles of prokaryotic origins. While progress has been made, the absence of atomic resolution structures for complexes of signal peptides and their receptors has definitely left many questions to be answered in the future. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90: 307–319, 2008.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Signal Peptide Conformations and Membrane Interactionsmentioning

confidence: 99%

Use of synthetic signal sequences to explore the protein export machinery

Clérico¹,

Maki²,

Gierasch³

2008

Biopolymers

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“…Strong linkage disequilibrium among six of the seven polymorphisms has been reported. Polymorphisms located in the signal peptide region regulate translation and͞or vectorial transport of proteins (28). In view of our findings that TGF-␤ 1 protein levels distinguished blacks from whites, we explored the hypothesis that codon 10 alleles, located in the signal peptide region of the TGF-␤ 1 gene, would distinguish blacks from whites.…”

Section: Increased Frequency Of Pro 10 Allele In Blacksmentioning

confidence: 99%

Transforming growth factor-beta 1 hyperexpression in African-American hypertensives: A novel mediator of hypertension and/or target organ damage

Suthanthiran

Li²,

Song³

et al. 2000

Proceedings of the National Academy of Sciences

192

126

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Hypertension, a remediable risk factor for stroke, cardiovascular disease, and renal failure, affects 50 million individuals in the United States alone. African Americans (blacks) have a higher incidence and prevalence of hypertension and hypertension-associated target organ damage compared with Caucasian Americans (whites). Herein, we explored the hypotheses that transforming growth factor-␤1 (TGF-␤1) is hyperexpressed in hypertensives compared with normotensives and that TGF-␤1 overexpression is more frequent in blacks compared with whites. These hypotheses were stimulated by our recent demonstration that TGF-␤1 is hyperexpressed in blacks with end-stage renal disease compared with white end-stage renal disease patients and by the biological attributes of TGF-␤1, which include induction of endothelin-1 expression, stimulation of renin release, and promotion of vascular and renal disease when TGF-␤ 1 is produced in excess. TGF-␤1 profiles were determined in black and white hypertensive subjects and normotensive controls and included circulating protein concentrations, mRNA steady-state levels, and codon 10 genotype. Our investigation demonstrated that TGF-␤1 protein levels are highest in black hypertensives, and TGF-␤1 protein as well as TGF-␤1 mRNA levels are higher in hypertensives compared with normotensives. The proline allele at codon 10 (Pro 10 ) was more frequent in blacks compared with whites, and its presence was associated with higher levels of TGF-␤1 mRNA and protein. Our findings support the idea that TGF-␤ 1 hyperexpression is a risk factor for hypertension and hypertensive complications and provides a mechanism for the excess burden of hypertension in blacks.

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“…Quenching data are summarized in Table 2 in the form of the ratio of the fluorescence intensity in unlabeled LUVs (IO) to that in labeled LUVs (I). An I0/I value of 1 The total lipid concentration in each experiment was 1 mM in 5 mM Tris, pH 7.3, and peptide concentrations were 5 uM. It is not appropriate to compare directly the values for the different quenchers, since accurate doxyl concentrations and quenching efficiencies are not known for the different positions.…”

Section: Resultsmentioning

confidence: 99%

“…The importance of the signal sequence in protein secretion is well established (1)(2)(3). Several lines of evidence also indicate that the mature portion of a secretory protein must meet certain criteria to be competent for secretion, and, further, that the signal sequence and its passenger must be compatible to be successful partners in the export pathway (4).…”

mentioning

confidence: 99%