Genetic analysis of partial resistance to basal stem rot (Sclerotinia sclerotiorum) in sunflower

Amouzadeh, Masoumeh; Darvishzadeh, Reza; Haddadi, Parham; Abdollahi, Mandoulakani Babak; Rezaee, Danesh Younes

doi:10.2298/gensr1303737a

Cited by 16 publications

(23 citation statements)

References 25 publications

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“…The development of an artificial inoculation method by Gulya et al (2008) provided the opportunity to evaluate a large number of sunflower lines for BSR in multiple field environments (Talukder et al, 2014b,c). Prior to our study, there were only two reports of QTL mapping for BSR resistance in a sunflower RIL mapping population, PAC2 × RHA266 (Davar et al, 2010; Amouzadeh et al, 2013). Both studies were conducted under controlled growth chamber conditions but with different fungal isolates.…”

Section: Discussionmentioning

confidence: 99%

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SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

Talukder¹,

Seiler

Song

et al. 2016

The Plant Genome

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Basal stalk rot (BSR), caused by the ascomycete fungus Sclerotinia sclerotiorum (Lib.) de Bary, is a serious disease of sunflower (Helianthus annuus L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 ´ RHA 439. A genotyping-bysequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401.36 cM. The RILs were tested in five environments (locations and years) for resistance to BSR. Quantitative trait loci were identified in each environment separately and also with integrated data across environments. A total of six QTL were identified in all five environments: one of each on LGs 4, 9, 10, 11, 16, and 17. The most significant QTL, Qbsr-10.1 and Qbsr-17.1, were identified at multiple environments on LGs 10 and 17, explaining 31.6 and 20.2% of the observed phenotypic variance, respectively. The remaining four QTL, .1, were detected in only one environment on LGs 4, 9, 11, and 16, respectively. Each of these QTL explains between 6.4 and 10.5% of the observed phenotypic variation in the RIL population. Alleles conferring increased resistance were contributed by both parents. The potential of the Qbsr-10.1 and Qbsr-17.1 in marker-assisted selection (MAS) breeding are discussed. Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungus with a vast host range of >400 broadleaf species (Boland and Hall, 1994). The fungus causes three distinctly different diseases on sunflower: BSR or wilt, midstalk rot (MSR), and head rot. This fungal pathogen is characterized by its ability to produce long-term survival structures called sclerotia, which individually consist of masses of hyphae surrounded by a hard, black, protective rind. Depending on environmental conditions, sclerotia can germinate myceliogenically, and cause root infection, or carpogenically, producing apothecia then ascospores and infect aboveground parts of host plants (Gulya et al., 1997;Bolton et al., 2006). Unlike other hosts, BSR symptoms start from a root infection resulting from myceliogenic germination of sclerotia. Midstalk rot commonly begins as a leaf infection, while head rot infection begins on capitula. Both MSR and head rot symptoms are incited by airborne ascospores Univ., Fargo, ND 58102, USA. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.

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Section: Discussionmentioning

confidence: 99%

“…2). Amouzadeh et al (2013) screened the same RIL population with fungal isolate SSKH41 and identified five QTL on LGs 1, 3, 8, 10, and 17. Among them, LGs 10 and 17 were common with our study, where we also identified BSR resistance QTL.…”

Section: Discussionmentioning

confidence: 99%

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

Talukder¹,

Seiler

Song

et al. 2016

The Plant Genome

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“…Notably, seven of the eight resistant introgression lines developed in this study had the alien segments detected in LG8, suggesting that new QTL of BSR resistance from the wild species are also present in this linkage group. The two QTL on LG10 detected by Amouzadeh et al (2013) and Talukder et al (2016) are located at a region between 253.4 and 281. 3 Mb, while ~34 polymorphic SNPs (66.7% of the shared SNPs) in LG10 that detected alien segments in three introgression lines were also located in this region (Table 8).…”

Section: Discussionmentioning

confidence: 85%

“…Amouzadeh et al (2013) reported QTL conferring partial resistance to BSR using a recombinant inbred line (RIL) population derived from a cross of PCA2/RHA 266. The five QTL for the percentage of necrotic area, based on controlled growth chamber tests, were located on LGs 1, 3, 8, 10, and 17 with the small effects, and each QTL explained between 0.5 and 3.2% of the observed phenotypic variance in the RIL population.…”

Section: Discussionmentioning

confidence: 99%

Genotyping-by-Sequencing Uncovers the Introgression Alien Segments Associated with Sclerotinia Basal Stalk Rot Resistance from Wild Species—I. Helianthus argophyllus and H. petiolaris

Long

Talukder

et al. 2016

Front. Genet.

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Basal stalk rot (BSR), caused by Sclerotinia sclerotiorum, is a devastating disease in sunflower worldwide. The progress of breeding for Sclerotinia BSR resistance has been hampered due to the lack of effective sources of resistance for cultivated sunflower. Our objective was to transfer BSR resistance from wild annual Helianthus species into cultivated sunflower and identify the introgressed alien segments associated with BSR resistance using a genotyping-by-sequencing (GBS) approach. The initial crosses were made between the nuclear male sterile HA 89 with the BSR resistant plants selected from wild Helianthus argophyllus and H. petiolaris populations in 2009. The selected resistant F1 plants were backcrossed to HA 458 and HA 89, respectively. Early generation evaluations of BSR resistance were conducted in the greenhouse, while the BC2F3 and subsequent generations were evaluated in the inoculated field nurseries. Eight introgression lines; six from H. argophyllus (H.arg 1 to H.arg 6), and two from H. petiolaris (H.pet 1 and H.pet 2), were selected. These lines consistently showed high levels of BSR resistance across seven environments from 2012 to 2015 in North Dakota and Minnesota, USA. The mean BSR disease incidence (DI) for H.arg 1 to H.arg 6, H.pet 1, and H.pet 2 was 3.0, 3.2, 0.8, 7.2, 7.7, 1.9, 2.5, and 4.4%, compared to a mean DI of 36.1% for Cargill 270 (susceptible hybrid), 31.0% for HA 89 (recurrent parent), 19.5% for HA 441 (resistant inbred), and 11.6% for Croplan 305 (resistant hybrid). Genotyping of the highly BSR resistant introgression lines using GBS revealed the presence of the H. argophyllus segments in linkage groups (LGs) 3, 8, 9, 10, and 11 of the sunflower genome, and the H. petiolaris segments only in LG8. The shared polymorphic SNP loci in the introgression lines were detected in LGs 8, 9, 10, and 11, indicating the common introgression regions potentially associated with BSR resistance. Additionally, a downy mildew resistance gene, Pl17, derived from one of the parents, HA 458, was integrated into five introgression lines. Germplasms combining resistance to Sclerotinia BSR and downy mildew represent a valuable genetic source for sunflower breeding to combat these two destructive diseases.

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“…Breeding using resistant germplasm is the most economic and efficient disease management strategy used by most sunflower breeding programs. However, BSR resistance breeding is relatively complex, as resistance is controlled by multiple genes, each with a small effect (Davar et al, 2010; Talukder et al, 2014b), some of which have been mapped to the sunflower genome and linked with DNA markers (Davar et al, 2010; Amouzadeh et al, 2013; Talukder et al, 2016). Although no sunflower hybrids are completely resistant to BSR, significant variation in tolerance to this disease has been reported in currently available germplasm resources (Talukder et al, 2014a).…”

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confidence: 99%

Registration of Oilseed Sunflower Germplasm HA‐BSR1 Highly Tolerant to Sclerotinia Basal Stalk Rot

Talukder

Seiler³

et al. 2017

J of Plant Registrations

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Basal stalk rot (BSR) is a devastating disease that causes a significant damage to sunflower (Helianthus annuus L.) production worldwide by reducing seed yield and quality. The objective of this research was to develop highly BSR tolerant sunflower germplasm by incorporating genetic factors from various partially tolerant breeding lines. HA‐BSR1 (Reg. No. GP‐336, PI 678571) sunflower germplasm is an F7–derived oilseed maintainer developed from the cross HA 441/RHA 439, selected for tolerance to Sclerotinia BSR. HA‐BSR1 showed significantly higher tolerance to BSR disease than both of the parents and the checks when evaluated across seven environments in North Dakota and Minnesota from 2012 to 2015. A 4‐yr mean disease incidence of HA‐BSR1 was 1.6% compared with the parents HA 441 (20.0%) and RHA 439 (12.6%), the susceptible hybrid check ‘Cargill 270’ (32.8%), and the resistant hybrid check ‘Croplan 305’ (10.4%). Molecular genetic analysis of HA‐BSR1 indicated the presence of six alleles associated with quantitative trait loci (QTL) conferring resistance against BSR, three alleles derived from each parents. The release of HA‐BSR1 along with the information of single nucleotide polymorphism markers linked to QTL will be useful in marker‐assisted selection breeding to pyramid BSR resistance with other desirable agronomic traits in elite sunflower lines.

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Genetic analysis of partial resistance to basal stem rot (Sclerotinia sclerotiorum) in sunflower

Cited by 16 publications

References 25 publications

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping‐by‐Sequencing

Genotyping-by-Sequencing Uncovers the Introgression Alien Segments Associated with Sclerotinia Basal Stalk Rot Resistance from Wild Species—I. Helianthus argophyllus and H. petiolaris

Registration of Oilseed Sunflower Germplasm HA‐BSR1 Highly Tolerant to Sclerotinia Basal Stalk Rot

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