Genetic analysis of parentage within experimental populations of a male dimorphic beetle, Onthophagus taurus, using amplified fragment length polymorphism

Simmons, Leigh W.; Beveridge, Maxine; Krauss, Siegfried L.

doi:10.1007/s00265-004-0843-0

Cited by 36 publications

(45 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amplified fragment length polymorphism (AFLP) fingerprinting can be used to determine parentage (Mueller & Wolfenbarger, 1999;Vos et al, 1995) and has recently been applied to some insect species (Garcia-Gonzalez et al, 2003Simmons et al, 2004). AFLPs were resolved according to the AFLP™ Plant Mapping Kit protocol (Perkin Elmer).…”

Section: Parentage Analysismentioning

confidence: 99%

“…All peaks were scored for presence/absence in each individual using the Genescan™ analysis software in the 50-500 bp range. All peaks with a height above 150 fluorescent units we considered for small fragments (Questiau et al, 1999), since two diagnostic peaks seem to be sufficient for detecting parentage in most cases ( Garcia-Gonzalez et al, 2003Questiau et al, 1999;Simmons et al, 2004). All the fragments present in the offspring and the two potential fathers and mothers were scored.…”

Section: Parentage Analysismentioning

confidence: 99%

See 1 more Smart Citation

Mating competition and parentage assessment in Ptomascopus morio (Coleoptera: Silphidae): A case for resource defense polygyny

Suzuki¹,

Nagano²,

Kobayashi³

2006

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract. Ptomascopus morio of both sexes are attracted to vertebrate carcasses, a necessary resource for reproduction. The stage during reproduction that resource defense was most intense and the hypothesis that large beetles were better competitors and sired a larger share of the offspring were supported and tested. Male-male aggression (pushing, biting and mounting) was commonly observed before and during oviposition, but rarely after the larvae hatched. Few female-female aggressive interactions were observed at any time. Parentage analysis of the offspring of six groups of two males and two females each reproducing on a separate carcass revealed that the large males sired more of the offspring than small males. Paternity analysis, using AFLP markers, revealed that larger males had higher paternity than smaller males, but the number of eggs produced by each female did not differ between large and small females. This suggests that competition among males is intense until the end of oviposition and that resident (large) males can acquire more mates and sire more offspring than smaller males; competition among females was not evident at any time.

show abstract

Section: Parentage Analysismentioning

confidence: 99%

Section: Parentage Analysismentioning

confidence: 99%

Mating competition and parentage assessment in Ptomascopus morio (Coleoptera: Silphidae): A case for resource defense polygyny

Suzuki¹,

Nagano²,

Kobayashi³

2006

Eur. J. Entomol.

View full text Add to dashboard Cite

show abstract

“…In male dimorphic species, minor or hornless males adopt the alternative mating tactic of sneaking copulations with females that are guarded by major or horned males (17,18,20). In the species that have been studied, a male's fertilization success depends on the amount of sperm inseminated relative to other males (21,22), an assumption made in sperm competition game theory (3), and the proportion of sneak males in a population is associated with both the relative sizes of the testes, and the numbers of sperm produced (23), suggesting that sperm competition favors large testes size in these beetles. Thus, onthophagine beetles appear to have experienced a history of selection for significant investment into both horns and testes and for this reason are ideal for testing for a developmental allocation trade-off between these traits.…”

mentioning

confidence: 99%

Evolutionary trade-off between weapons and testes

Simmons

Emlen

2006

Proc. Natl. Acad. Sci. U.S.A.

273

255

View full text Add to dashboard Cite

It has long been recognized that male mating competition is responsible for the evolution of weaponry for mate acquisition. However, when females mate with more than one male, competition between males can continue after mating in the form of sperm competition. Theory predicts that males should increase their investment in sperm production as sperm competition is increased, but it assumes that males face a trade-off between sperm production and other life-history traits such as mate acquisition. Here, we use a genus of horned beetle, Onthophagus, to examine the trade-off between investment in testes required for fertilizations and investment in weapons used to obtain matings. In a within-species study, we prevented males from developing horns and found that these males grew larger and invested relatively more in testes growth than did males allowed to grow horns. Among species, there was no general relationship between the relative sizes of horns and testes. However, the allometric slope of horn size on body size was negatively associated with the allometric slope of testes size on body size. We suggest that this reflects meaningful evolutionary changes in the developmental mechanisms regulating trait growth, specifically in the degree of nutrition-dependent phenotypic plasticity versus canalization of traits. Finally, we show how this resource allocation trade-off has influenced the evolutionary diversification of weapons, revealing a rich interplay between developmental trade-offs and both preand postmating mechanisms of sexual competition.secondary sexual traits ͉ sperm competition ͉ testes size ͉ beetle horns

show abstract

“…While needing more pipetting steps, our two-step protocol could be performed in two 30 min incubations, whereas the usual double digest performed in a single-step in most AFLP protocols is much more time consuming, requiring an incubation for 2-3 h (Vos et al, 1995;Behura et al, 2000;Simmons et al, 2004). An initial typical result of the two-step digestion protocol performed on DNA extracted with the high-salt protocol is illustrated in Figure 1B.…”

Section: Aflp Protocol Optimizationmentioning

confidence: 99%

“…We initiated this study with a commercially available and frequently employed system (AFLP Analysis system I, Invitrogen) which has been successfully used in a number of insect species, such as the gall midge Orseolia oryzae (Behura et al, 2000;Katiyar et al, 2000), the fruit fly Ceratitis capitata (Corsini et al, 1999), the damselfly Nehalennia irene (Wong et al, 2001), the sweet potato whitefly, Bemisia tabaci (Göçmen and Devran, 2002), the dung beetle Onthophagus taurus (Simmons et al, 2004) and also the honey bee, Apis mellifera (Suazo and Hall, 1999;Rueppell et al, 2004). Since we could not obtain reproducible results (consistent bands using the same DNA extracts as templates) with this system in the stingless bee M. quadrifasciata, even after testing different strategies of DNA extraction to obtain DNA of adequate purity for an insect AFLP study (Reineke et al, 1998), we decided to go through a complete optimization process of the AFLP reactions with this species.…”

Section: Introductionmentioning

confidence: 99%

An optimized method for the generation of AFLP markers in a stingless bee (Melipona quadrifasciata) reveals a high degree of intracolonial genetic polymorphism

2006

View full text Add to dashboard Cite

-This study describes an optimized protocol for the generation of Amplified Fragment Length Polymorphism (AFLP) markers in a stingless bee. Essential modifications to standard protocols are a restriction enzyme digestion (EcoRI and Tru1I) in a two-step procedure, combined with a touchdown program in the selective PCR amplification step and product labelling by incorporation of α[ 33 P]dATP. In an analysis of 75 workers collected from three colonies of Melipona quadrifasciata we obtained 719 markers. Analysis of genetic variability revealed that on average 32% of the markers were polymorphic within a colony. Compared to the overall percentage of polymorphism (44% of the markers detected in our bee samples), the observed rates of within-colony polymorphism are remarkably high, considering that the workers of each colony were all offspring of a singly mated queen. amplified fragment length polymorphism / genotyping / stingless bee / genetic polymorphism / Melipona / Apidae

show abstract

Genetic analysis of parentage within experimental populations of a male dimorphic beetle, Onthophagus taurus, using amplified fragment length polymorphism

Cited by 36 publications

References 65 publications

Mating competition and parentage assessment in Ptomascopus morio (Coleoptera: Silphidae): A case for resource defense polygyny

Mating competition and parentage assessment in Ptomascopus morio (Coleoptera: Silphidae): A case for resource defense polygyny

Evolutionary trade-off between weapons and testes

An optimized method for the generation of AFLP markers in a stingless bee (Melipona quadrifasciata) reveals a high degree of intracolonial genetic polymorphism

Contact Info

Product

Resources

About