Genetic analysis of floral anthocyanin pigmentation traits in Asiatic hybrid lily using molecular linkage maps

Abstract

To understand the genetic background of two floral anthocyanin pigmentation traits, anthocyanin pigmentation in the flower tepals and spot formation, in the Asiatic hybrid lily (2n = 24), segregation of the two traits among 96 F(1) plants derived from a cross between commercial cultivars 'Montreux' and 'Connecticut King' were investigated. 'Montreux' has anthocyanin pigmentation in the tepals with many spots, and 'Connecticut King' has flowers with carotenoid pigmentation without spots. The F(1) plants with or without anthocyanin pigment in the tepals segregated with a 1:1 segregation ratio, indicating that a single gene controls anthocyanin pigmentation in the tepals. The number of spots per square centimeter of all tepals showed continuous distribution in the F(1) plants. To map the loci for the two anthocyanin pigmentation traits, molecular linkage maps in the Asiatic hybrid lily were constructed using a double pseudo-testcross strategy, with the same F(1) plants used for phenotypic evaluation, and 212 PCR-based DNA markers. The trait for anthocyanin pigmentation in tepals was used as a trait marker. The map of 'Montreux' comprised 95 markers in 26 linkage groups, and the map of 'Connecticut King' used 119 markers in 24 linkage groups. The total map lengths were 867.5 and 1,114.8 cM, respectively. The trait locus for anthocyanin pigmentation in the tepals was between markers ASR35-180 and P506-40 in linkage group 1 of the 'Montreux' map with a map distance of 1.2 cM and 2.6 cM, respectively. A single-point analysis of quantitative trait loci (QTLs) for tepal spot number identified two putative QTLs in linkage groups 1 and 19 of the 'Connecticut King' map. One putative QTL in linkage group 19 explained 64% of the total phenotypic variation. Because both putative QTLs were mapped on the linkage map of 'Connecticut King' that has no spots, dominant alleles of them might suppress spot formation.

Genetic analysis and associated SRAP markers for flowering traits of chrysanthemum (Chrysanthemum morifolium)

“…Although we were unable to estimate the contribution of polygene and environmental variation, as is possible using several jointly segregating families Zeng and Pu 2006), the detection of major genes for initial blooming time and duration of flowering of chrysanthemum is a useful start to identifying molecular markers associated with these traits. (Abe et al 2002;Dugo et al 2005;Dunemann et al 1999;Remay et al 2009;Yagi et al 2006). In chrysanthemum, in contrast, there has been less progress to date in this direction (Zhang et al 2010b;Zhao et al 2009a, b).…”

“…This requires a single segregating generation and several jointly segregating families (Wang and Gai 1997;Zhang et al 2000), and has been successfully applied to the genetic analysis of a number of agronomically important quantitative traits Guo et al 2006;Hao et al 2008b;Zeng and Pu 2006;Zhang et al 2006). Quantitative trait locus (QTL) analysis has been attempted in the ornamental species lily (Abe et al 2002), rose (Dugo et al 2005;Oyant et al 2008) and carnation (Yagi et al 2006), and genetic linkage maps of chrysanthemum have recently been constructed (Zhang et al 2010b). Among the major methods of QTL analysis, marker-based variance analysis has become the preferred means where only limited genetic linkage map information is available-such as in the tree species poplar (Huang et al 2004a, b;Su et al 2000), Taxus yunnanensis (Su et al 2009) and Camellia sinensis (Yao et al 2010).…”

Two distinct spontaneous mutations involved in white flower development in Lilium speciosum

“…The total anthocyanins in the tepals, anthers, and buds were determined using the method of Abe et al (2002), and total carotenoids in the anthers were determined according to the methods of Nakano et al (2005) and Yamagishi et al (2010a). Briefly, anthocyanins and carotenoids were extracted using a 5 % formic acid and acetone solution, and pigment concentrations were determined by measuring the absorbance at A 515 or A 437 for anthocyanins or carotenoids, respectively, using a spectrophotometer.…”

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

“…This is due to the fact that lily is a vegetative propagated bulbous plant, and from crossing to anthesis, it takes more than one year. But studies have rarely been conducted to clarify the genetic bases of the important agronomic traits in lily (Abe et al 2002). Therefore, it is of great practical importance to shorten the breeding cycle so as to improve the breeding efficiency.…”