Genetic analysis of broad spectrum resistance to potyviruses using doubled haploid lines of pepper (Capsicum annuum L.)

Abstract

Genetic analysis of resistance to PVY in androgenetic doubled haploid lines, Fl, F2 and backcross progenies of the Mexican pepper line, CM 334 (Capsicum annuum L.), was performed . Three reaction types were observed when seedlings were inoculated with several PVY strains of different pathotypes and with an American PeMV strain . Resistant genotypes never showed systemic symptoms although some individuals sporadically developed necrotic local lesions on inoculated cotyledons . Susceptible genotypes exhibited either a typical systemic mosaic or a systemic necrosis that caused the death of the inoculated seedlings . Segregation analyses indicated that resistance to pepper potyviruses in CM 334 is conferred by two genes . The first one, tentatively named Pr4, is dominant and confers the resistance to all now known pathotypes of PVY and to PeMV The second one, tentatively named pry, is recessive ; it confers only the resistance to common strains of PVY The systemic necrotic response is conferred by an independent dominant gene, tentatively named Pnl . 231

Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.)

“…The data presented in Table 1 suggest that the resistance carried by the variety 'SCM-334' against the PVY pathotype 1-2 is due to a unique and dominant gene described as Pvr4 (Dogimont et al 1996).…”

“…Subsequently, some oligogenic resistances were also reported (Nagai and Costa 1972). Later, a monogenic dominant gene named Pvr4, that confers resistance against all known PVY pathotypes and pepper mottle virus (PepMoV), was found in the Capsicum annuum L. line 'Serrano Criollo de Morelos-334' (SCM-334) (Pasko et al 1992;Dogimont et al 1996). Recently, a new dominant source of resistance, Pvr7, has been described in Capsicum chinense Jacq.…”

New inheritance studies related to Potato Virus Y (PVY) resistance in Capsicum annuum L. ‘Serrano Criollo de Morelos-334’

“…Some of these resistances are oligogenic or polygenic (Caranta & Palloix, 1996) and two monogenic and dominant resistances have also been reported (Dogimont et al, 1996;Grube et al, 2000).…”

Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea

“…Dogimont et al (1996) reported that cultivars Vat and CM334 contain the lethal systemic necrosis factor Pn1. When inoculated with Potato virus Y (PVY) P(0) and PVY (1,2), Vat developed lethal necrosis, whereas CM334 developed the typical hypersensitive-response type of resistance.…”