Genetic Analysis of a Soybean Genetic Pool using ISSR Marker: Effect of Gamma Radiation on Genetic Variability

Mudibu, J.; Nkongolo, K. K.; Mehes-Smith, M.; Kalonji-Mbuyi, A.

doi:10.3923/ijpbg.2011.235.245

Cited by 33 publications

(29 citation statements)

References 10 publications

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“…The dose of gamma rays radiation is important for inducing genetic variation that can lead to positive mutant. Previous studies in soybean has shown that gamma ray irradiation at a dose of 0.2 kGy gamma rays effectively lead to genetic diversity in plants Mudibu et al [3] demonstrated using ISSR markers that the level of polymorphic loci observed within soybean varieties was significantly increased by more than 10 % when 0.2 kGy gamma-rays treatment was compared with the control. Hanafiah et al [6] reported also that the highest genetic variation at M2 generation of soybean was on 0.2 kGy doses.…”

Section: Discussionmentioning

confidence: 99%

“…The US, Brazil, Argentina, China and India are the world's largest soybean producers and represent more than 90% of global soybean production. The majority of African countries growing soybeans use tropical soybean varieties developed by the International Institute of tropical Agriculture (ITTA) [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…Genetic variability can also be increased by inducing mutations with ionized radiations [3,15,16]. Genetic diversity of mutant lines can be monitored using morphological, agronomic and molecular characterizations.…”

Section: Introductionmentioning

confidence: 99%

“…Genetic diversity of mutant lines can be monitored using morphological, agronomic and molecular characterizations. In addition, the effectiveness of gamma radiation in improving plant growth, seed quality, cooking time and physiological processes is highly related to the level of doses used [3,17,18]. For example, Hezazi and Hamideldin [17] showed that gamma irradiation at 0.4 kGy gave the highest effect in improving okra plant growth and seed yield compared to 0.3 kGy and 0.5 kGy.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (Glycine max L.)

Mudibu¹,

Nkongolo²,

Kalonji-Mbuyi³

et al. 2012

AJPS

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Mutation breeding in crop plants is an effective approach in improvement of crop having narrow genetic base such as soybean. The main objective of the present study is to determine the effect of different doses of gamma irradiation on different morpho-agronomic characteristics. Agronomic traits that were analyzed included; grain yield, number of pods/plant, number of seeds/plant and weight of 100 seeds and numbers of days to 50% flowering. Morphometric characterization of the descriptive data included plant height, stem diameter, number of leaves/plant, leaflet length, leaflet width, number of ramifications/plant, and pod length and width at 3 lodge stage. The results of the present study revealed that the two gamma irradiation doses used (0.2 kGy and 0.4 kGy) decreased significantly most of agronomic and morphological traits evaluated in M1 populations. Different effects of 0.2 kGy and 0.4 kGy irradiation were observed in M2 populations with significant increase of grain yields and yield components in all the three soybean varieties. In general, a significant decrease or no changes of morphological traits were observed for the two irradiation doses in M2 populations. The levels of changes varied among varieties. Potential high yielding mutants were identified in progenies of irradiated seeds

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (Glycine max L.)

Mudibu¹,

Nkongolo²,

Kalonji-Mbuyi³

et al. 2012

AJPS

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“…Mutation breeding supplements conventional plant breeding as a source of increasing variability and could confer specific improvement without significantly altering its phenotype [1]. The successful utilization of gamma rays to generate genetic variability in plant breeding has been reported in soybean [2][3][4] and other crops [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Groundnut (Arachis hypogaea L.)

Tshilenge-Lukanda¹,

Kalonji-Mbuyi²,

Nkongolo³

et al. 2013

AJPS

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Induced mutation in plant improvement has been used in several crops to generate new sources of genetic variations. A study was conducted to determine the effect of different doses of gamma irradiation on different morpho-agronomic characteristics. Agronomic traits that were analyzed included: grain yield, number of pods/plant, number of seeds/plant and weight of 100 seeds and numbers of days to 50% flowering. Morphometric characterisation of the descriptive data included plant height, stem diameter, number of leaves/plant, leaflet length, leaflet width and number of ramification/ plant. Groundnut seeds were treated with various doses of gamma rays (100, 200, 400 and 600 Gy). Among the various dose treatments, gamma rays treatment at 100 Gy resulted in a higher increase of grain yield and other morpho-agronomic parameters especially for the JL24 variety. In fact the gamma irradiation at 100 Gy increased significantly grain yield by 14% for JL24, and 4 % for JL12. The number of pods per plant was increased by 2% for JL12 and 37% for JL24. For the number of seeds per plant, there was a significant increase of 8% for JL12, and 62% for JL24 at 100 Gy. A similar trend was observed for the JL24 at 200 Gy dose. Higher doses of gamma rays (400 and 600 Gy) reduced significantly plant growth and grain yield. The usefulness of the mutants identified in a groundnut breeding program is discussed.

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Determination of the effective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed

Yarar

Koçak

Denli³

et al. 2021

Mol Biol Rep

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Plant breeding allows altering the genetic structure of plants to meet human needs. The use of radiation technology for inducing mutations and -thereby-new phenotypic variants has become increasingly common as a tool for developing new crops. The aim of this study was to determine the effective gamma irradiation dose for inducing mutations in purple carrot. For this purpose, increasing gamma radiation doses [0, 50, 100, 200, 300, 400, 500, and 600 Gy] were applied to purple carrot seeds. The irradiated seeds were sown in pots and the emergence and survival rates of the seedlings were analyzed. Considering plant emergence (%) as a response variable, the LD 50 dose was 387.5 Gy. Analysis of root length, root width (shoulder diameter) and plant height in control (0 Gy) and irradiated plants (50-600 Gy) revealed an inverse association between these morphological traits and radiation dose. SRAP and ISSR markers were used to identify DNA polymorphisms in irradiated and control plants. The range of amplicons per primer set revealed by ISSR and SRAP markers was 4-10 and 2-13, respectively. In ISSR analysis, the average Nei's gene diversity, Shannon's information index, and PIC value ranged from 0.13 to 0.25, from 0.20 to 0.35, from 1.39 to 1.67 for eight doses, respectively. In SRAP analysis, the average Nei's gene diversity, Shannon's information index, and PIC value were ranged from 0.15 to 0.25, from 0.23 to 0.37, from 0.43 to 0.58 for eight doses, respectively. Cluster analysis revealed three main groups; a) non-irratidated (control) plants, b) plants from the 600 Gy dose, and c) a third group with two subgroups: one with individuals from the lowest irradiation doses (50-200 Gy) and a second group with individuals from the highest irradiation doses (300-500 Gy).

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Genetic Analysis of a Soybean Genetic Pool using ISSR Marker: Effect of Gamma Radiation on Genetic Variability

Cited by 33 publications

References 10 publications

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (<i>Glycine max</i> L.)

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (<i>Glycine max</i> L.)

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Groundnut (<i>Arachis hypogaea</i> L.)

Determination of the effective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed

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Genetic Analysis of a Soybean Genetic Pool using ISSR Marker: Effect of Gamma Radiation on Genetic Variability

Cited by 33 publications

References 10 publications

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (&lt;i&gt;Glycine max&lt;/i&gt; L.)

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (&lt;i&gt;Glycine max&lt;/i&gt; L.)

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Groundnut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.)

Determination of the effective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed

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Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (<i>Glycine max</i> L.)

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (<i>Glycine max</i> L.)

Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Groundnut (<i>Arachis hypogaea</i> L.)