Genes essential for morphological development and antibiotic production in <i>Streptomyces coelicolor</i> are targets of BldD during vegetative growth

Hengst, Chris D. den; Tran, Ngat T.; Bibb, Maureen J.; Chandra, Govind; Leskiw, Brenda K.; Buttner, Mark J.

doi:10.1111/j.1365-2958.2010.07338.x

Cited by 175 publications

(276 citation statements)

References 84 publications

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“…The master regulators in the whi family, which are believed crucial for sporulation of aerial hyphae, have also been identified in the core genome: WhiG (strep4938) which encodes a sigma factor; WhiH (strep5046) which encodes a repressor; WhiI (strep5113) which encodes a two-component regulator; WhiA (strep3138), WhiB (strep3685) and WhiD (strep4478). Two other sigma factors that are important for sporulation, BldD (strep2821) [62] and SigF (strep4128) [63] are also present in the core genome [26]. …”

Section: Resultsmentioning

confidence: 99%

Genome plasticity and systems evolution in Streptomyces

Zhou

et al. 2012

BMC Bioinformatics

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BackgroundStreptomycetes are filamentous soil-dwelling bacteria. They are best known as the producers of a great variety of natural products such as antibiotics, antifungals, antiparasitics, and anticancer agents and the decomposers of organic substances for carbon recycling. They are also model organisms for the studies of gene regulatory networks, morphological differentiation, and stress response. The availability of sets of genomes from closely related Streptomyces strains makes it possible to assess the mechanisms underlying genome plasticity and systems adaptation.ResultsWe present the results of a comprehensive analysis of the genomes of five Streptomyces species with distinct phenotypes. These streptomycetes have a pan-genome comprised of 17,362 orthologous families which includes 3,096 components in the core genome, 5,066 components in the dispensable genome, and 9,200 components that are uniquely present in only one species. The core genome makes up about 33%-45% of each genome repertoire. It contains important genes for Streptomyces biology including those involved in gene regulation, secretion, secondary metabolism and morphological differentiation. Abundant duplicate genes have been identified, with 4%-11% of the whole genomes composed of lineage-specific expansions (LSEs), suggesting that frequent gene duplication or lateral gene transfer events play a role in shaping the genome diversification within this genus. Two patterns of expansion, single gene expansion and chromosome block expansion are observed, representing different scales of duplication.ConclusionsOur results provide a catalog of genome components and their potential functional roles in gene regulatory networks and metabolic networks. The core genome components reveal the minimum requirement for streptomycetes to sustain a successful lifecycle in the soil environment, reflecting the effects of both genome evolution and environmental stress acting upon the expressed phenotypes. A better understanding of the LSE gene families will, on the other hand, bring a wealth of new insights into the mechanisms underlying strain-specific phenotypes, such as the production of novel antibiotics, pathogenesis, and adaptive response to environmental challenges.

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Section: Resultsmentioning

confidence: 99%

Genome plasticity and systems evolution in Streptomyces

Zhou

et al. 2012

BMC Bioinformatics

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“…Following early genomic analyses predicting that GGDEF, EAL, and HD-GYP domains are involved in c-di-GMP metabolism in a variety of bacteria (27,34,107), experimental evidence for c-di-GMP signaling pathways was obtained for the major phylogenetic branches, including the Proteobacteria, Spirochetes, Cyanobacteria, Deinococcus-Thermus, Thermotogae, Actinobacteria, and Firmicutes (59,181,182). In the past several years, genome sequencing has revealed domains associated with c-di-GMP signaling even in the phyla previously thought to be devoid of c-di-GMP.…”

Section: Cyclic Di-gmp In Genomic Context Cyclic Di-gmp Signaling Enzmentioning

confidence: 99%

“…These hyphae undergo a sporulation-like cell division process, resulting in an aerial mycelium comprised of prespores, each of which contains only one set of genomic DNA, that subsequently metamorphose into mature spores (322). den Hengst et al first discovered that overexpression of the DGC designated CdgA (SCO2817) blocks formation of aerial hyphae and results in a bald colony appearance (182). Overexpression of yet another DGC, CdgB (SCO4281), resulted in the impaired formation of aerial mycelium.…”

Section: Regulation Of Cell Cycle and Differentiationmentioning

confidence: 99%

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

Römling

Galperin

Gomelsky

2013

Microbiol Mol Biol Rev

1,474

1,698

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SUMMARY Twenty-five years have passed since the discovery of cyclic dimeric (3′→5′) GMP (cyclic di-GMP or c-di-GMP). From the relative obscurity of an allosteric activator of a bacterial cellulose synthase, c-di-GMP has emerged as one of the most common and important bacterial second messengers. Cyclic di-GMP has been shown to regulate biofilm formation, motility, virulence, the cell cycle, differentiation, and other processes. Most c-di-GMP-dependent signaling pathways control the ability of bacteria to interact with abiotic surfaces or with other bacterial and eukaryotic cells. Cyclic di-GMP plays key roles in lifestyle changes of many bacteria, including transition from the motile to the sessile state, which aids in the establishment of multicellular biofilm communities, and from the virulent state in acute infections to the less virulent but more resilient state characteristic of chronic infectious diseases. From a practical standpoint, modulating c-di-GMP signaling pathways in bacteria could represent a new way of controlling formation and dispersal of biofilms in medical and industrial settings. Cyclic di-GMP participates in interkingdom signaling. It is recognized by mammalian immune systems as a uniquely bacterial molecule and therefore is considered a promising vaccine adjuvant. The purpose of this review is not to overview the whole body of data in the burgeoning field of c-di-GMP-dependent signaling. Instead, we provide a historic perspective on the development of the field, emphasize common trends, and illustrate them with the best available examples. We also identify unresolved questions and highlight new directions in c-di-GMP research that will give us a deeper understanding of this truly universal bacterial second messenger.

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“…5 The bldD gene product, BldD, is a small DNA-binding protein with 167 amino acids and exists predominantly as a homodimer in solution. [6][7][8] BldD binds its own promoter and regulatory regions of several genes, 6,7,9,10 including bldN encoding a sigma factor involved in aerial hyphae formation, 11 whiG encoding a sigma factor related in spore formation 12 and sigH encoding a sigma factor associated with stress responses. 13 The transcription levels of these target genes in the bldD mutant in comparison with those of the wild type have shown that BldD operates as a transcriptional repressor.…”

Section: Introductionmentioning

confidence: 99%

“…13 The transcription levels of these target genes in the bldD mutant in comparison with those of the wild type have shown that BldD operates as a transcriptional repressor. 9,10,13 BldD has two structurally independent domains, an N-terminal domain (residues 1-79, BldD-NTD), which is responsible for both the DNA-binding and dimerization, and a C-terminal domain (residues 80-167, BldD-CTD), of which function is unknown. 8 Although the crystal structure of BldD-NTD showed a classical helix-turn-helix motif for DNA binding and a remarked structural similarity with lambda repressor, 14 it was not sufficient to fully explain the regulatory mechanism of BldD, without structural information of the BldD-CTD.…”

Section: Introductionmentioning

confidence: 99%

Mainchain NMR Assignments and secondary structure prediction of the C-terminal domain of BldD, a developmental transcriptional regulator from Streptomyces coelicolor A3(2)

Kim¹,

Won²,

Kang³

2013

Journal of the Korean Magnetic Resonance Society

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BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [ 13 C/ 15 N]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a β-α-α-β-α-α-α topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

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Genes essential for morphological development and antibiotic production in Streptomyces coelicolor are targets of BldD during vegetative growth

Abstract: SummaryBldD is a transcriptional regulator essential for morphological development and antibiotic production in

Cited by 175 publications

References 84 publications

Genome plasticity and systems evolution in Streptomyces

Genome plasticity and systems evolution in Streptomyces

Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger

Mainchain NMR Assignments and secondary structure prediction of the C-terminal domain of BldD, a developmental transcriptional regulator from Streptomyces coelicolor A3(2)

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