Generation of Neural Progenitor Cells from Whole Adult Bone Marrow

Kabos, Peter; Ehtesham, Moneeb; Kabosova, Andrea; Black, Keith L.; Yu, John S.

doi:10.1006/exnr.2002.8039

Cited by 134 publications

(85 citation statements)

References 18 publications

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“…CD90, OCT4, Musashi-1 (MSI), NOTCH4, JAG2, and DLL-1 are typically expressed in embryonic or adult stem cells (Kaneko et al, 2000;Kabos et al, 2002;Nagato et al, 2005). We found that expression levels of these stem cell-associated genes were higher in PASC1 than in its differentiated daughter cells (Figure 3A a).…”

Section: Expression Of Stem Cell-associated Genes By Pascs and Chemosmentioning

confidence: 82%

Isolation of tumour stem-like cells from benign tumours

et al. 2009

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BACKGROUND: Cancerous stem-like cells (CSCs) have been implicated as cancer-initiating cells in a range of malignant tumours. Diverse genetic programs regulate CSC behaviours, and CSCs from glioblastoma patients are qualitatively distinct from each other. The intrinsic connection between the presence of CSCs and malignancy is unclear. We set out to test whether tumour stem-like cells can be identified from benign tumours. METHODS: Tumour sphere cultures were derived from hormone-positive and -negative pituitary adenomas. Characterisation of tumour stem-like cells in vitro was performed using self-renewal assays, stem cell-associated marker expression analysis, differentiation, and stimulated hormone production assays. The tumour-initiating capability of these tumour stem-like cells was tested in serial brain tumour transplantation experiments using SCID mice. RESULTS: In this study, we isolated sphere-forming, self-renewable, and multipotent stem-like cells from pituitary adenomas, which are benign tumours. We found that pituitary adenoma stem-like cells (PASCs), compared with their differentiated daughter cells, expressed increased levels of stem cell-associated gene products, antiapoptotic proteins, and pituitary progenitor cell markers. Similar to CSCs isolated from glioblastomas, PASCs are more resistant to chemotherapeutics than their differentiated daughter cells.

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Section: Expression Of Stem Cell-associated Genes By Pascs and Chemosmentioning

confidence: 82%

Isolation of tumour stem-like cells from benign tumours

et al. 2009

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“…The marrow was flushed and the tissue was passed through a mesh to remove small pieces of debris. After washing, nestin-positive spheres were obtained as described previously by our group (Kabos et al 2002). Cells were plated at a density of 1!10 6 cells/well in poly-D lysinecoated 24-well plates (BD Biosciences, San Jose, CA, USA) in serum-free DMEM/F12 medium (Invitrogen), supplemented with 20% B27 (Invitrogen), 20 ng/ml of fibroblast growth factor (bFGF, Peprotech, Rocky Hill, NJ, USA), 20 ng/ml of epidermal growth factor (EGF, Peprotech), penicillin (100 U/ml) and streptomycin (100 mg/ml).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

“…We previously demonstrated that a subpopulation of nestin-expressing cells, isolated from bone marrow is able to generate cellular spheres similar to neurospheres derived from brain neural stem cells. These cells from bone marrow could differentiate into all three neural phenotypes (neurons, astrocytes, and oligodendrocytes) in vitro and in vivo (Kabos et al 2002, Zeng et al 2007. Recent studies showed that bone marrow-derived stem cells could reverse the hyperglycemic phenotype in a diabetic animal model but the mechanism behind the rescue or regeneration of pancreatic islets is still debated (Hasegawa et al 2007, Xu et al 2007, Gao et al 2008, Zhao et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of nestin-positive cells derived from bone marrow into pancreatic endocrine and ductal cells in vitro

Milanesi

Lee²,

Xu³

et al. 2011

Journal of Endocrinology

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Promising results of pancreatic islet transplantation to treat type 1 diabetes mellitus, combined with severe shortage of donor pancreata, have spurred efforts to generate pancreatic islet-like cells and insulin-producing b-cells from various progenitor populations. In this study, we show for the first time that multipotent nestin-positive stem cells selected from rat bone marrow can be differentiated into pancreatic ductal and insulin-producing b-cells in vitro. We report an effective multistep protocol in a serum-free system, which could efficiently induce b-cell differentiation from multipotent nestin-positive bone marrow stem cells. To enhance the induction and differentiation toward pancreatic lineage we used trichostatin A, an important regulator of chromatin remodeling, and 5-aza 2 0 deoxycytidine, an inhibitor of DNA methylase. All-trans retinoic acid was then utilized to promote pancreatic differentiation. We sequentially induced important transcription factor genes, such as Pdx1, Ngn3, and Pax6, following the in vivo development timeline of the pancreas in rats. Furthermore, in the final stage with the presence of nicotinamide, the induced cells expressed islet and ductal specific markers. The differentiated cells not only expressed insulin and glucose transporter 2, but also displayed a glucoseresponsive secretion of the hormone. Our results delineate a new model system to study islet neogenesis and possible pharmaceutical targets. Nestin-positive bone marrow stem cells may be therapeutically relevant for b-cell replacement in type 1 diabetes.

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“…Different agents, especially cytokines, growth factors, neurotrophins, and retinoic acid have been shown to promote neural cell induction in vitro [31,39]. In addition, some other regimens based on chemical inductive mediums including dimethyl sulfoxide (DMSO) [33] and butylated hydroxyanisole (BHA), or agents that elevate intracellular cAMP levels [35], promote MSC neuronal differentiation.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Somatic stem cell research for neural repair: current evidence and emerging perspectives

Corti

Locatelli

Papadimitriou

et al. 2004

J Cellular Molecular Medi

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Recent evidence supports the existence of adult mammalian stem cell subpopulations, particularly within the bone marrow, that may be able to "transdifferentiate" across tissue lineage boundaries, thus offering an accessible source for therapeutic applications even for neural tissue repair. However, the difficulties in reproducing some experimental data, the rarity of the transdifferentiation events and observations that cell fusion may be an alternative explanation argue against the idea of stem cell plasticity. Investigations going beyond descriptive experiments and more mechanicistic approaches may provide a more solid foundation to adult stem cell therapeutic potential.

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Generation of Neural Progenitor Cells from Whole Adult Bone Marrow

Cited by 134 publications

References 18 publications

Isolation of tumour stem-like cells from benign tumours

Isolation of tumour stem-like cells from benign tumours

Differentiation of nestin-positive cells derived from bone marrow into pancreatic endocrine and ductal cells in vitro

Somatic stem cell research for neural repair: current evidence and emerging perspectives

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