Generation of matched patient-derived xenograft in vitro-in vivo models using 3D macroporous hydrogels for the study of liver cancer

Fong, Eliza Li Shan; Toh, Tan Boon; Lin, Quy Xiao Xuan; Liu, Zheng; Hooi, Lissa; Rashid, Masturah Bte Mohd Abdul; Benoukraf, Touati; Chow, Edward Kai‐Hua; Huynh, The Hung; Yu, Hanry

doi:10.1016/j.biomaterials.2017.12.026

Cited by 59 publications

(63 citation statements)

References 48 publications

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“…Due to their interconnected, macroporous 3D structure, cryogels have been widely used in bioseparation‐related applications [1–10], tissue engineering [10–14], and other relevant bioengineering and sensor applications [15–17]. In particular, monolithic cryogels have been used as a new generation of chromatographic matrices for the separation of cells (mammalian, bacterial, and yeast), proteins, viruses, and plasmids [18–22].…”

Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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A novel, facile, and robust strategy was proposed to increase the pore size and mechanical strength of cryogels. By mixing the monomers of acrylamide and 2‐hydroxyethyl methacrylate as the precursor, a monolithic copolymer cryogel with large interconnected pores and thick pore walls was prepared. Hydrogen bonding between the two monomers contributed to the entanglement and aggregation of the copolymers, thickening the pore walls and resulting in larger pore sizes. Analysis via mercury porosimetry demonstrated that the interconnected pore diameter of the copolymer cryogel ranged from 10‐350 µm, which was far larger than that of the cryogels from one monomer (10‐50 µm). Additionally, the thicker pore walls of the copolymer cryogel improved its mechanical strength. Affinity cryogels were prepared through covalent immobilization using Tris(hydroxymethyl)aminomethane as a coupling agent, and the affinity binding of lysozymes on Tris‐cryogel was evaluated by the Langmuir isothermal adsorption with the maximum adsorption capacity of 360 mg/g. Compared with that of the Tris‐cryogels produced from one monomer, the copolymer Tris‐cryogel exhibited higher adsorption capacity and lysozyme purity, when the chicken egg white solution flowed solely driven by gravity. This work provides a new avenue for designing and developing supermacroporous cryogels for bioseparation.

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Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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“…Currently, researchers use biopsy or surgical resection of tumor samples for detection, but if relevant test factors can be found in the blood, it would be of great significance in determining the clinical effectiveness and responsiveness of immunotherapy. In vitro research models need to be closer to the state of the body, such as organoids incorporating the immune microenvironment (89,90). Meanwhile, organoids can better reveal tumor progression and drug resistance and contribute to anticancer drug screening in the future (90).…”

Section: Discussionmentioning

confidence: 99%

“…In vitro research models need to be closer to the state of the body, such as organoids incorporating the immune microenvironment (89,90). Meanwhile, organoids can better reveal tumor progression and drug resistance and contribute to anticancer drug screening in the future (90).…”

Section: Discussionmentioning

confidence: 99%

Progress and Challenges in Precise Treatment of Tumors With PD-1/PD-L1 Blockade

Jiang

Zhao

et al. 2020

Front. Immunol.

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Immune checkpoint inhibitors target the inhibitory receptors on T cells to reinstate their antitumor ability and have shown significant efficacy in treating various cancers. However, because of tumor heterogeneity and many other uncover reasons, the objective response rate for programmed death 1 and programmed death-ligand 1 (PD-1/PD-L1) blockade is only 20 to 30%; its response rate in solid tumors is relatively low, and different degrees of side effects have occurred. There are still many unknown factors affecting the therapeutic effectiveness of PD-1/PD-L1 blockade. Additionally, screening the responding tumor patients accurately and improving the response rate and efficacy are huge challenges for tumor precise treatment. Here, we attempt to summarize the recent progress in response prediction and combined application of PD-1/PD-L1 blockade and briefly discuss the methods and evaluations combined with PD-1/PD-L1 blockade to improve the implementation of precision immunotherapy.

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“…They demonstrated the benefits of this model in promoting the understanding of liver cancer biology and in developing personalized‐medicine approaches for the disease. Moreover, Fong et al generated a 3D macroporous sponge system to maintain in vivo‐like metabolic activities of a tumor model based on the combination of hepatocellular carcinoma (HCC) cells and a patient‐derived xenograft (PDX). They demonstrated that this sponge system was able to support the rapid organoid formation of HCC‐PDX cells and maintain their viability and proliferative capacity.…”

Section: Conventional Versus Advanced Processing Technologies For LIVmentioning

confidence: 99%

“…Therefore the development of models, like organoids, which could well mimic the in vivo conditions through self‐organization has emerged. In order to create liver organoids, advanced processing technologies, such as 3D Bioprinting, have been applied . However, recent models have been developed based on the self‐organization of the cells, resulting in the development of “scaffold‐free” organoids.…”

Section: Conventional Versus Advanced Processing Technologies For LIVmentioning

confidence: 99%

Advanced Biomaterials and Processing Methods for Liver Regeneration: State‐of‐the‐Art and Future Trends

Morais

Vieira

Zhao

et al. 2020

Adv Healthcare Materials

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Liver diseases contribute markedly to the global burden of mortality and disease. The limited organ disposal for orthotopic liver transplantation results in a continuing need for alternative strategies. Over the past years, important progress has been made in the field of tissue engineering (TE). Many of the early trials to improve the development of an engineered tissue construct are based on seeding cells onto biomaterial scaffolds. Nowadays, several TE approaches have been developed and are applied to one vital organ: the liver. Essential elements must be considered in liver TE—cells and culturing systems, bioactive agents or growth factors (GF), and biomaterials and processing methods. The potential of hepatocytes, mesenchymal stem cells, and others as cell sources is demonstrated. They need engineered biomaterial‐based scaffolds with perfect biocompatibility and bioactivity to support cell proliferation and hepatic differentiation as well as allowing extracellular matrix deposition and vascularization. Moreover, they require a microenvironment provided using conventional or advanced processing technologies in order to supply oxygen, nutrients, and GF. Herein the biomaterials and the conventional and advanced processing technologies, including cell‐sheets process, 3D bioprinting, and microfluidic systems, as well as the future trends in these major fields are discussed.

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Generation of matched patient-derived xenograft in vitro-in vivo models using 3D macroporous hydrogels for the study of liver cancer

Cited by 59 publications

References 48 publications

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Progress and Challenges in Precise Treatment of Tumors With PD-1/PD-L1 Blockade

Advanced Biomaterials and Processing Methods for Liver Regeneration: State‐of‐the‐Art and Future Trends

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