DOI: 10.1038/protex.2017.155
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Abstract: This protocol describes procedures for generating cell lines in which the MT plus end tracking protein EB1 is replaced by a photo-inactivated variant (π-EB1), which we generated by inserting a light sensitive protein interaction module between functional domains of EB1. π-EB1 photo-dissociation allows control of microtubule dynamics with high spatial and temporal accuracy and local investigation of microtubule functions in complex cell behaviors. Here we describe two protocols we have used to functionally repl…

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