Generation and characterization of three induced pluripotent stem cells lines from an 86-year old female individual diagnosed with an invasive lobular mammary carcinoma

Co-cultures of iPSC-derived Mammary-like Organoids and Patient-derived Microtumors Model Invasive Behavior of Breast Cancer ex vivo

“…CD34 + hematopoietic stem and progenitor cells were isolated from peripheral blood mononuclear cells (PBMCs) from two patients diagnosed with an invasive lobular carcinoma and reprogrammed into iPSCs with non-integrative episomal vectors (Thermo Fisher Scienti c, USA). The iPSCs were characterized comprehensively as recently described [15,16]. For the co-culture with BCP 1 PDMs, BCP 3 PDMs and MDA-MB-231 spheroids, MLOs derived from the cell line NMIi011-A were used (here referred to as "MLO 1") [16].…”

“…For the co-culture with BCP 1 PDMs, BCP 3 PDMs and MDA-MB-231 spheroids, MLOs derived from the cell line NMIi011-A were used (here referred to as "MLO 1") [16]. NMIi012-B derived MLOs (here named "MLO 2") were used for the co-culture with BCP 2 PDMs and MCF-7 spheroids [15]. Notably, NMIi012-B iPSCs were generated from PBMCs of BCP 2.…”

“…In the present study, we introduce a three-dimensional (3D) co-culture system of patient-derived breast microtumors or cell line-derived spheroids (hereafter summarized as "breast cancer spheres") and mammary-like organoids (MLOs), which allows to investigate the in uence of the mammary (myo-) epithelium on tumor growth, invasion, and metastasisrelated processes in an autologous and allogenic manner. We recently generated induced pluripotent stem cells (iPSCs) from peripheral blood of two breast cancer patient (BCP) diagnosed with invasive lobular carcinomas whose microtumors were included in this study [15,16]. Here, we differentiated the iPSCs into functional MLOs expressing markers of the mammary epithelium and myoepithelium.…”