volume 66, issue 7, P4339-4347 1992
DOI: 10.1128/jvi.66.7.4339-4347.1992
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H Y Lee, P J Krell

Abstract: We have generated defective genomes of Autographa californica nuclear polyhedrosis virus (AcNPV) by serial, undiluted passage in IPLB-SF-21 cell culture in an attempt to identify potential cis-acting sequences important for AcNPV DNA replication. Viral DNA isolated from some of the 81 serial passages was analyzed by pulsed-field gel electrophoresis, restriction endonuclease analysis, and Southern blot hybridization. AcNPV-defective genomes appeared to be generated through a series of successively smaller and t…

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