2007
DOI: 10.1534/genetics.106.066837
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Abstract: The maize transposable element Activator (Ac) has been exploited as an insertional mutagen to disrupt, clone, and characterize genes in a number of plant species. To develop an Ac-based mutagenesis platform for maize, a large-scale mutagenesis was conducted targeting the pink scutellum1 locus. We selected 1092 Ac transposition events from a closely linked donor Ac, resulting in the recovery of 17 novel ps1 alleles. Multiple phenotypic classes were identified corresponding to Ac insertions in the 59-UTR and cod… Show more

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Cited by 34 publications
(39 citation statements)
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“…The resulting ears were screened using one of two methods. When the ps1-m8TAc allele was used as a donor Ac, colorless progeny ears were screened visually for segregation of a ps1 mutant phenotype with a lack of Ac activity (Singh et al 2003;Bai et al 2007). Progeny ears from all other donor Ac elements were screened using a molecular assay.…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…The resulting ears were screened using one of two methods. When the ps1-m8TAc allele was used as a donor Ac, colorless progeny ears were screened visually for segregation of a ps1 mutant phenotype with a lack of Ac activity (Singh et al 2003;Bai et al 2007). Progeny ears from all other donor Ac elements were screened using a molecular assay.…”
Section: Methodsmentioning
confidence: 99%
“…Ac donor lines were selected from a subset of previously characterized insertions (Kolkman et al 2005) and from an Ac insertion at the pink scutellum1 (ps1) locus, ps1-m8TAc, which conditions a weak mutant phenotype when homozygous (Bai et al 2007). Ac activity was visualized as purple sectors on the kernels resulting from excision of a Ds element from the r1 locus during kernel development (r1-sc:m3; see materials and methods).…”
Section: Genetic Selection For Newly Formed Ds Derivativesmentioning
confidence: 99%
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“…A second project involves the use of the Activator (Ac)/Dissociation (Ds) transposable element system in a strategy of targeted, regional mutagenesis. Exploiting the propensity of Ac and Ds to transpose short distances, this strategy aims to saturate specific chromosomal regions adjacent to mapped Ac/Ds transposons in an effort to generate new insertional mutations in genes closely linked to existing transposons (Bai et al 2007). Once identified, new Ac/Ds insertional mutations generate a molecular tag that is utilized for cloning targeted genes (Pohlman et al 1984).…”
Section: Genetics and Genomicsmentioning
confidence: 99%
“…Once identified, new Ac/Ds insertional mutations generate a molecular tag that is utilized for cloning targeted genes (Pohlman et al 1984). Imprecise transposon excision/insertion events can create "footprint" alleles, which generate allelic variation and contribute to genome evolution (Chen et al 1987;Kolkman et al 2005;Bai et al 2007). Characterized by an increased transposon copy number and a correspondingly elevated mutation rate, the Mutator (Mu) transposable element system has emerged as a popular method for mutagenesis among maize geneticists.…”
Section: Genetics and Genomicsmentioning
confidence: 99%