General Approach for Monitoring Peptide–Protein Interactions Based on Graphene–Peptide Complex

Lü, Chunhua; Li, Juan; Zhang, Xiaolong; Zheng, Aixian; Yang, Huanghao; Chen, Xi

doi:10.1021/ac200617k

Cited by 89 publications

(44 citation statements)

References 54 publications

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“…13À15 The currently available fluorescent approaches to study proteinÀpeptide interactions are largely based on green fluorescent protein, 16 fluorophore/quencher dual-labeled peptides, 17À19 or quencher/fluorophorelabeled peptide complex. 20 The requirement of dual modification of peptides or the use of complexes adds to the cost and complexity of the probe design and assay operation. In addition, most of the existing fluorescence assays for a proteinÀpeptide interaction study do not operate in living organisms.…”

mentioning

confidence: 99%

Self-Assembly-Induced Far-Red/Near-Infrared Fluorescence Light-Up for Detecting and Visualizing Specific Protein–Peptide Interactions

Wang¹,

Liu

Han³

et al. 2014

ACS Nano

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Understanding specific protein-peptide interactions could offer a deep insight into the development of therapeutics for many human diseases. In this work, we designed and synthesized a far-red/near-infrared (FR/NIR) fluorescence light-up probe (DBT-2EEGWRESAI) by simply integrating two tax-interacting protein-1 (TIP-1)-specific peptide ligands (EEGWRESAI) with one 4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole (DBT) unit. We first demonstrated that DBT is an environment-sensitive fluorophore with FR/NIR fluorescence due to its strong charge transfer character in the excited state. Thanks to the environmental sensitivity of DBT, the probe DBT-2EEGWRESAI is very weakly fluorescent in aqueous solution but lights up its fluorescence when the probe specifically binds to TIP-1 protein or polyprotein (ULD-TIP-1 tetramer). It is found that the DBT-2EEGWRESAI/TIP-1 protein and the DBT-2EEGWRESAI/ULD-TIP-1 tetramer could self-assemble into spherical nanocomplexes and a nanofiber network, respectively, which lead to probe fluorescence turn-on through providing DBT with a hydrophobic microenvironment. By virtue of the self-assembly-induced FR/NIR fluorescence turn-on, DBT-2EEGWRESAI can detect and visualize specific protein/polyprotein-peptide interactions in both solution and live bacteria in a high contrast and selective manner.

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confidence: 99%

Self-Assembly-Induced Far-Red/Near-Infrared Fluorescence Light-Up for Detecting and Visualizing Specific Protein–Peptide Interactions

Wang¹,

Liu

Han³

et al. 2014

ACS Nano

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“…Thus, many GO-based fluorescent chem/bio-sensors have been developed for monitoring the enzymatic activities [34,35,36,37,38,39], measuring the levels of various analytes including nucleic acids, proteins, metal ions and small molecules [40,41,42,43,44], and imaging of cells as well as animals [45,46]. Based on the high quenching ability of GO and the specific aptamer–target interaction, several groups have reported the detection of proteins (e.g., thrombin, cyclin A2, amyloid-β oligomers, α-bungarotoxin and antibodies) with the dye-labeled DNA or peptide probes as the recognition elements [47,48,49,50,51]. In a typical detection model, the fluorescence of a dye-labeled probe would be quenched when it was absorbed onto the surface of GO.…”

Section: Introductionmentioning

confidence: 99%

A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin

Xia

Wang

Liu

2016

Sensors

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Human chorionic gonadotropin (hCG) has been regarded as a biomarker for the diagnosis of pregnancy and some cancers. Because the currently used methods (e.g., disposable Point of Care Testing (POCT) device) for hCG detection require the use of many less stable antibodies, simple and cost-effective methods for the sensitive and selective detection of hCG have always been desired. In this work, we have developed a graphene oxide (GO)-based fluorescent platform for the detection of hCG using a fluorescein isothiocyanate (FITC)-labeled hCG-specific binding peptide aptamer (denoted as FITC-PPLRINRHILTR) as the probe, which can be manufactured cheaply and consistently. Specifically, FITC-PPLRINRHILTR adsorbed onto the surface of GO via electrostatic interaction showed a poor fluorescence signal. The specific binding of hCG to FITC-PPLRINRHILTR resulted in the release of the peptide from the GO surface. As a result, an enhanced fluorescence signal was observed. The fluorescence intensity was directly proportional to the hCG concentration in the range of 0.05–20 IU/mL. The detection limit was found to be 20 mIU/mL. The amenability of the strategy to hCG analysis in biological fluids was demonstrated by assaying hCG in the urine samples.

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“…With the numerous reports of the many exceptional properties and applications of CNTs and fullerenes, the intensive research of graphene and its potential use as a nanometer-scale building block and fertile ground for analytical purpose, is expected. Especially, incorporation of light sbsorbing antenna chromophores through a covalent or noncovalent linkage with the extended π electrons of graphene sheets would constitute an ideal supramolecular nanoassembly, which has been considered to be important to tune optoelectronic properties of graphene [6]–[8] and enlarge the field of graphene-based analytical application [9]–[12].…”

Section: Introductionmentioning

confidence: 99%

Obstruction of Photoinduced Electron Transfer from Excited Porphyrin to Graphene Oxide: A Fluorescence Turn-On Sensing Platform for Iron (III) Ions

2012

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A comparative reaserch of the assembly of different porphyrin molecules on graphene oxide (GO) and reduced graphene oxide (RGO) was carried out, respectively. Despite the cationic porphyrin molecules can be assembled onto the surfaces of graphene sheets, including GO and RGO, to form complexes through electrostatic and π-π stacking interactions, the more obvious fluorescence quenching and the larger red-shift of the Soret band of porphyrin molecule in RGO-bound states were observed than those in GO-bound states, due to the differenc of molecular flattening in degree. Further, more interesting finding was that the complexes formed between cationic porphyrin and GO, rather than RGO sheets, can facilitate the incorporation of iron (III) ions into the porphyrin moieties, due to the presence of the oxygen-contained groups at the basal plane of GO sheets served as auxiliary coordination units, which can high-efficiently obstruct the electron transfer from excited porphyrin to GO sheets and result in the occurrence of fluorescence restoration. Thus, a fluorescence sensing platform has been developed for iron (III) ions detection in this contribution by using the porphyrin/GO nanohybrids as an optical probe, and our present one exhibited rapid and sensitive responses and high selectivity toward iron (III) ions.

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General Approach for Monitoring Peptide–Protein Interactions Based on Graphene–Peptide Complex

Cited by 89 publications

References 54 publications

Self-Assembly-Induced Far-Red/Near-Infrared Fluorescence Light-Up for Detecting and Visualizing Specific Protein–Peptide Interactions

Self-Assembly-Induced Far-Red/Near-Infrared Fluorescence Light-Up for Detecting and Visualizing Specific Protein–Peptide Interactions

A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin

Obstruction of Photoinduced Electron Transfer from Excited Porphyrin to Graphene Oxide: A Fluorescence Turn-On Sensing Platform for Iron (III) Ions

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