Gene sequences and specific detection for Panton-Valentine leukocidin

Nakagawa, Saori; Taneike, Ikue; Mimura, Daigo; Iwakura, Nobuhiro; Nakayama, Teruko; Emura, Tomoko; Kitatsuji, Maki; Fujimoto, Atsushi; Yamamoto, Tatsuo

doi:10.1016/j.bbrc.2005.01.054

Cited by 94 publications

(60 citation statements)

References 21 publications

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“…DNA extraction was performed as previously described (34). The presence of Panton-Valentine leukocidin was assessed using real-time multiplex PCR assays as previously described (13,30). Staphylococcal cassette chromosome mec (SCCmec) typing was performed by the method of Oliveira et al (32).…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Risk Factor Analysis for Methicillin-Resistant Staphylococcus aureus Nasal Colonization in Children Attending Child Care Centers

et al. 2011

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Children attending child care centers (CCCs) are at increased risk for infections, including those caused by methicillin-resistant Staphylococcus aureus (MRSA). Nasal colonization often precedes infection, and MRSA colonization has been associated with increased infection risk. Community-associated MRSA (CA-MRSA) has caused increased MRSA infections in the general population, including children. Little is known about the frequency of MRSA nasal colonization in young children, particularly in those attending CCCs where disease transmission is common. We sampled the nares of 1,163 children in 200 classrooms from 24 CCCs in North Carolina and Virginia to assess S. aureus colonization. MRSA strains were molecularly analyzed for staphylococcal cassette chromosome mec (SCCmec) type, Panton-Valentine leukocidin status, and multilocus sequence type. A case-control study was performed to identify risk factors for MRSA colonization. We found that 18.1% children were colonized with S. aureus and 1.3% with MRSA. Molecular analysis of the MRSA strains identified 47% as CA-MRSA and 53% as health care-associated MRSA (HA-MRSA). Although two centers had multiple children colonized with MRSA, genotyping indicated that no transmission had occurred within classrooms. The case-control study did not detect statistically significant risk factors for MRSA colonization. However, MRSA-colonized children were more likely to be nonwhite and to have increased exposure to antibiotics and skin infections in the home. Both CA-MRSA and HA-MRSA strains were found colonizing the nares of children attending CCCs. The low frequency of colonization observed highlights the need for a large multicenter study to determine risk factors for MRSA colonization and subsequent infection in this highly susceptible population.

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Section: Methodsmentioning

confidence: 99%

Prevalence and Risk Factor Analysis for Methicillin-Resistant Staphylococcus aureus Nasal Colonization in Children Attending Child Care Centers

et al. 2011

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“…The complete DNA sequence of lukS-PV and lukF-PV genes was investigated using PCR primers described by Nakagawa et al (2005). The following conditions were used for cycle sequencing of the genes: 1 min at 96°C, 30 cycles of 10 s at 96°C, 5 s at 50°C (for lukSforward), 5 s at 61°C (for lukS-reverse), 5 s at 48°C (lukF-forward), 5 s at 58°C (lukF-reverse) and 4 min at 60°C.…”

Section: Sequencing Of Luks-pv and Lukf-pv Genesmentioning

confidence: 99%

Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton–Valentine Leukocidin gene

Sudağıdan

Aydın

2010

International Journal of Food Microbiology

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“…The presence of PVL was assessed using real-time PCR assays targeting pvlS and pvlF (12) and the intergenic region between pvlS and pvlF. The assay was done using 200 nM of the probe (5Ј FAM-CTC ATG AAA TTA AAG TGA AAG GAC-TAMRA, where FAM is 6-carboxyfluorescein and TAMRA is 6-carboxytetramethylrhodamine) and 900 nM of each primer (forward, 5Ј AAC AGA AAT TAC ACA GTT A; reverse, 5Ј ATT GAT GTA ACA ACT GAT GAT).…”

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Prevalence of Community-Associated Methicillin-Resistant Staphylococcus aureus in Patients with Cystic Fibrosis

et al. 2009

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We prospectively determined the prevalence of community-associated Staphylococcus aureus in a large cystic fibrosis (CF) center between October 2005 and October 2007. We found that 2.7% (19/707) of the CF patients who had cultures during the study period were infected with this organism, representing 14% of the total methicillin-resistant Staphylococcus aureus strains ( n = 140) recovered from the patient population during the study period.

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Gene sequences and specific detection for Panton-Valentine leukocidin

Cited by 94 publications

References 21 publications

Prevalence and Risk Factor Analysis for Methicillin-Resistant Staphylococcus aureus Nasal Colonization in Children Attending Child Care Centers

Prevalence and Risk Factor Analysis for Methicillin-Resistant Staphylococcus aureus Nasal Colonization in Children Attending Child Care Centers

Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton–Valentine Leukocidin gene

Prevalence of Community-Associated Methicillin-Resistant Staphylococcus aureus in Patients with Cystic Fibrosis

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