Gene regulation in response to DNA damage

Mannuss, Anja; Trapp, Oliver; Puchta, Holger

doi:10.1016/j.bbagrm.2011.08.003

Cited by 66 publications

(58 citation statements)

References 235 publications

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“…Under conditions of acute X-ray irradiation, the gene AtRad1 is regulated independently of AtKu70 and AtRAD51. Thus, it can be concluded that expression of inducible gene AtRAD51 and partly inducible gene AtKu70 is governed by the same transcriptional factor SOG1 [2,5]. Regarding AtRad1, investigation confirms the regulation of its expression by SOG1-independent mechanism, as indicated by the observation that transcription activity of AtRad1 under X-ray exposure do not match by activity of the genes AtKu70 and AtRAD51.…”

Section: Co-regulation Of Transcriptional Response Of Genes Participsupporting

confidence: 53%

“…3). Specific transcription factors, such as suppressor of gamma response 1 (SOG1) [2,5], and RNA-interference can be considered as potential regulators of transcription of these genes.…”

Section: Radiation-induced Expression Of Atku70 Andmentioning

confidence: 99%

“…Therefore, it can be assumed that the particular modification of radiosensitivity of plant cells under the influence of radiation might be caused by the change in the transcriptional activity of genome caretaker genes in a manner resembling those found in mammalian cells [5][6][7].…”

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confidence: 99%

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The Transcriptional Response of Arabidopsis thaliana L. Genes AtKu70, AtRAD51 and AtRad1 to X-Ray Radiation

Litvinov¹,

Rashydov²

2017

JAST-A

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Abstract:The study of influence of the fractionated and acute ionizing radiation on plants revealed that it is able to induce genomic instability. The hypothesis that transcription rate of several evolutionary conserved DNA repair genes AtKu70, AtRAD51 and AtRad1, which keeps genome stability in cells of model plant Arabidopsis thaliana, changes differently depending on dose and mode of ionizing radiation exposure had been tested. Gel electrophoresis-based reverse transcription polymerase chain reaction (RT-PCR) method was used for quantifying mRNA transcription levels. The data demonstrated that mode and dose of irradiation affect transcription rate of the genes AtKu70, AtRAD51 and AtRad1. The fractionated and acute X-ray irradiation may result in adaptive response through the induction of key DNA double-strand break (DSB) repair genes AtKu70 and AtRAD51, as well as in genome instability through transcriptional activation of error-prone AtRad1-mediated DNA DSB repair combined with decreased expression of AtRAD51. In plants at doses within the range of 3-9 Gy, an adaptive influence is prevailed, but at doses of 12-21 Gy an error-prone repair of double-strand DNA damage is activated. Fractionation of dose has a significant effect on the transcription of the genes AtKu70, AtRAD51 and AtRad1 only at doses of 15 Gy and 21 Gy. Acute dose of 15 Gy activates error-prone AtRad1-mediated DSB repair and repressed both AtRAD51-dependent and AtKu70-dependent repair pathways, while fractionated irradiation at the same total dose induces more accurate homologous recombination and canonical non-homologous end joining of the DNA strands. In case of A. thaliana exposed to X-rays at dose 21 Gy, the situation is going reversed because of strong induction of the all three genome caretaker genes in leaves of acutely irradiated plants in contrast to the plants under fractionated exposure.

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Section: Co-regulation Of Transcriptional Response Of Genes Participsupporting

confidence: 53%

“…3). Specific transcription factors, such as suppressor of gamma response 1 (SOG1) [2,5], and RNA-interference can be considered as potential regulators of transcription of these genes.…”

Section: Radiation-induced Expression Of Atku70 Andmentioning

confidence: 99%

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The Transcriptional Response of Arabidopsis thaliana L. Genes AtKu70, AtRAD51 and AtRad1 to X-Ray Radiation

Litvinov¹,

Rashydov²

2017

JAST-A

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“…We will not however cover in any detail ecological and agronomical aspects of the impact of UV-B on plants, as well as UV-B stress signaling (i.e. where UV-B-mediated damage is perceived), for which the reader is referred to the recent literature (Britt 2004, Ballare et al 2011, Ballare et al 2012, Mannuss et al 2012, Gonzalez Besteiro and Ulm 2013, Wargent and Jordan 2013.…”

Section: Uv-bmentioning

confidence: 99%

The UVR8 UV-B Photoreceptor: Perception, Signaling and Response

Tilbrook

Arongaus

Binkert

et al. 2013

The Arabidopsis Book

230

187

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Ultraviolet-B radiation (UV-B)is an intrinsic part of sunlight that is accompanied by significant biological effects. Plants are able to perceive UV-B using the UV-B photoreceptor UVR8 which is linked to a specific molecular signaling pathway and leads to UV-B acclimation. Herein we review the biological process in plants from initial UV-B perception and signal transduction through to the known UV-B responses that promote survival in sunlight. The UVR8 UV-B photoreceptor exists as a homodimer that instantly monomerises upon UV-B absorption via specific intrinsic tryptophans which act as UV-B chromophores. The UVR8 monomer interacts with COP1, an E3 ubiquitin ligase, initiating a molecular signaling pathway that leads to gene expression changes. This signaling output leads to UVR8-dependent responses including UV-B-induced photomorphogenesis and the accumulation of UV-B-absorbing flavonols. Negative feedback regulation of the pathway is provided by the WD40-repeat proteins RUP1 and RUP2, which facilitate UVR8 redimerization, disrupting the UVR8-COP1 interaction. Despite rapid advancements in the field of recent years, further components of UVR8 UV-B signaling are constantly emerging, and the precise interplay of these and the established players UVR8, COP1, RUP1, RUP2 and HY5 needs to be defined. UVR8 UV-B signaling represents our further understanding of how plants are able to sense their light environment and adjust their growth accordingly.

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“…Paraquat was considered to be slightly toxic to adult bees (FAO, 2003), Cousin et al (2013) 65 showed the disruption in larval development of oenocytes at very low concentrations, as well as 66 DNA damage (Lehmann, 2005;Mannuss et al, 2012). 67…”

mentioning

confidence: 99%

Estudo do melhoramento genético no sistema produtivo de pólen apícola e suas implicações na saúde das abelhas <i>Apis mellifera </i>L<i>.</i>

Mattos¹

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Estudo do melhoramento genético no sistema produtivo de pólen apícola e suas implicações na saúde das abelhas Apis mellifera L. Ribeirão Preto -SP 2016 IGOR MEDICI DE MATTOSEstudo do melhoramento genético no sistema produtivo de pólen apícola e suas implicações na saúde das abelhas Apis mellifera L. À Dra. Emily Griffith pela ajuda no processamento dos dados estatísticos e desenvolvimento do modelo matemático. À Raquel Lunardi Baccetto pela ajuda na elaboração deste texto, bem como pela disponibilidade e apoio.A todos os colegas do Boco A do Departamento de Genética e também do Laboratório de Biologia e Genética de Abelhas (LBGA), que aqui, por receio de cometer injustiças, agradeço coletivamente.Meu especial "MUITO OBRIGADO" ao técnico Jairo de Souza por toda dedicação, disponibilidade, orientação, confiança e amizade.Por fim, a todos que, de alguma forma, contribuíram direta e indiretamente para a realização desse trabalho e conviveram comigo durante esses 4 anos, meus sinceros agradecimentos. A produção de pólen apícola vem experimentando uma rápida e empolgante expansão desde o final da década de 1990. Tal crescimento pode ser explicado pelo aumento do interesse popular em alimentos de alto valor nutricional, bem como outras inúmeras aplicações desse produto na produção animal. Atualmente em torno de 1.500 toneladas de pólen são produzidas anualmente em todo o mundo. Apesar da importância econômica, social e ecológica assumida por esse setor da cadeia produtiva apícola, poucos estudos vêm abordando o tema, desde o fim da década de 1960. Tal desinteresse científico fez com que diversos aspectos relacionados às técnicas de manejo mais adequadas permanecessem pouco entendidos ou até mesmo nunca validados. Como agravante, um acentuado declínio populacional vem sendo percebido nas espécies de polinizadores, em especial A. mellifera. Apesar da gravidade e recorrência, tal fenômeno ainda é pouco entendido. Inúmeros agentes parecem exercer algum tipo de influencia negativa na biologia e fisiologia das abelhas melíferas (pesticidas, patógenos, ectoparasitas e perda de habitat), porém nenhum desses fatores foi comprovadamente identificado como desencadeador. Hoje a hipótese emergente, mais aceita entre os pesquisadores da área, trata do possível efeito sinergístico desses fatores como possível desencadeador dessa síndrome. É nesse contexto que estudos relativos à sanidade dessas abelhas se tornaram essenciais para a conservação da espécie e de seus serviços ecológicos. O pólen como imunoefetor e mitigador do estresse oxidativo se tornou, portanto, uma das mais proeminentes alternativas para proteção das abelhas A. mellifera. A pesquisa teve como principal objetivo a obtenção de informações que propiciassem o melhoramento das práticas vigentes de produção de pólen apícola, além do melhor entendimento do efeito do pólen no sistema imunológico de abelhas melíferas (analisado através do estudo da prevalência de patógenos) e no sistema de defesa antioxidante desses insetos. Este estudo traz informações importantes sobre o...

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Gene regulation in response to DNA damage

Cited by 66 publications

References 235 publications

The Transcriptional Response of Arabidopsis thaliana L. Genes AtKu70, AtRAD51 and AtRad1 to X-Ray Radiation

The Transcriptional Response of Arabidopsis thaliana L. Genes AtKu70, AtRAD51 and AtRad1 to X-Ray Radiation

The UVR8 UV-B Photoreceptor: Perception, Signaling and Response

Estudo do melhoramento genético no sistema produtivo de pólen apícola e suas implicações na saúde das abelhas <i>Apis mellifera </i>L<i>.</i>

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