Gene regulation by Y-box proteins: coupling control of transcription and translation

Matsumoto, Ken; Wolffe, Alan P.

doi:10.1016/s0962-8924(98)01300-2

Cited by 282 publications

(244 citation statements)

References 51 publications

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“…The Y-box-binding protein 1 (YB-1), which is a DNA/ RNA-binding protein also known as dbpB, regulates transcription, translation, DNA damage repair and other biological processes in both the nucleus and cytoplasm (Matsumoto and Wolffe, 1998;Kohno et al, 2003). In the cytoplasm, YB-1 regulates mRNA stability and translational regulation (Evdokimova et al, 2001;Ashizuka et al, 2002;Fukuda et al, 2004), while in the nucleus, it plays a pivotal role in transcriptional regulation through specific recognition of the Y-box promoter element (Ladomery and Sommerville, 1995;Kohno et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells

et al. 2006

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Y-box-binding protein 1 (YB-1), which is a member of the DNA-binding protein family containing a cold-shock domain, has pleiotropic functions in response to various environmental stimuli. As we previously showed that YB-1 is a global marker of multidrug resistance in ovarian cancer and other tumor types. To identify YB-1-regulated genes in ovarian cancers, we investigated the expression profile of YB-1 small-interfering RNA (siRNA)-transfected ovarian cancer cells using a high-density oligonucleotide array. YB-1 knockdown by siRNA upregulated 344 genes, including MDR1, thymidylate synthetase, S100 calcium binding protein and cyclin B, and downregulated 534 genes, including CXCR4, N-myc downstream regulated gene 1, E-cadherin and phospholipase C. Exogenous serum addition stimulated YB-1 translocation from the cytoplasm to the nucleus, and treatment with Akt inhibitors as well as Akt siRNA and integrin-linked kinase (ILK) siRNA specifically blocked YB-1 nuclear localization. Inhibition of Akt activation downregulated CXCR4 and upregulated MDR1 (ABCB1) gene expression. Administration of Akt inhibitor resulted in decrease in nuclear YB-1-positive cancer cells in a xenograft animal model. Akt activation thus regulates the nuclear translocation of YB-1, affecting the expression of drug-resistance genes and other genes associated with the malignant characteristics in ovarian cancer cells. Therefore, the Akt pathway could be a novel target of disrupting the nuclear translocation of YB-1 that has important implications for further development of therapeutic strategy against ovarian cancers.

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Section: Introductionmentioning

confidence: 99%

Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells

et al. 2006

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“…The CSPs are eukaryotic homologues of bacterial cold-shock proteins, which function as RNA chaperones (Matsumoto and Wolffe, 1998). Human heat-shock proteins have been well studied; however, much is not known about the functional roles of cold-shock proteins.…”

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confidence: 99%

Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen

et al. 2006

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Y-box-binding proteins are members of the human cold-shock domain protein superfamily, which includes dbpA, dbpB/YB-1, and dbpC/contrin. dbpC/contrin is a germ cell-specific Y-box-binding protein and is suggested to function as a nuclear transcription factor and RNA-binding protein in the cytoplasm. Whereas ubiquitous dbpB/YB-1 expression has been well studied in various types of human carcinomas as a prognostic or predictive marker, the dbpC/contrin expression in human tumour cells has not been reported. In this report, we provide the first evidence showing that dbpC was highly expressed in human testicular seminoma and ovarian dysgerminomas, and in carcinomas in other tissues and that its expression in normal tissues is nearly restricted to germ cells and placental trophoblasts. These results indicate that dbpC/contrin would be a potentially novel cancer/testis antigen.

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“…The Y-box binding proteins are multifunctional regulators of gene expression (Matsumoto & Wolffe 1998) and can bind to both double-stranded and single-stranded DNA and also to mRNA (Franco et al 1997a). Twentynine sequences of this gene were found in libraries of males, females, adult worms and cercariae.…”

Section: Discussionmentioning

confidence: 99%

Clustering of Schistosoma mansoni mRNA sequences and analysis of the most transcribed genes: implications in metabolism and biology of different developmental stages

Prosdocimi

Faria-Campos

Peixoto

et al. 2002

Mem. Inst. Oswaldo Cruz

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Key words: Schistosoma mansoni -bioinformatics -expressed sequences tag -clustering analysis -metabolismSchistosoma mansoni is a dioiceous trematode and one of the etiologic agents of schistosomiasis, the second more significant tropical disease concerning public health. Despite recent efforts undertaken to contain its progress, the disease is still endemic in several countries, with around 200 million people infected by the parasite (http://www.who.int/ctd/schisto/epidemio.htm). The study of S. mansoni is, therefore, very important in human parasitology. Gaining knowledge on the genome of this parasite is essential for a better understanding of its metabolism and biology and will help to elucidate important aspects of the mechanisms of drug resistance and antigenic variation that allow it to escape from the host immune system (Franco et al. 2000).The size of S. mansoni genome is estimated in 270Mb with the number of expressed genes ranging from 15000 to 20000 (Simpson et al. 1982, Franco & Simpson 2001. Although some genomic sequences of S. mansoni have been produced, the Schistosoma Genome Network (SGN) has chosen as first priority the sequencing of cDNA using the expressed sequence tags (ESTs) strategy, from which is possible to obtain fast and relevant information Although resulting in fast and very important information, ESTs available from public databases, such as dbEST, show some degree of redundancy and present a great number of errors, because they are single pass sequences (Miller et al. 1999). To overcome these problems and to increase the length of the sequences, facilitating identification by homology searches, clustering procedures are performed (Oliveira & Johnston 2001). In this kind of procedure, sequences that have some region of similarity are joined into a cluster. Therefore, sequences possessing overlapping regions and representing a single gene are joined into the same cluster, decreasing redundancy. Sequences of each cluster are then aligned to generate a consensus sequence. In this approach, the base (and, if available, the quality value designated by the base caller program) present in each sequence position is considered in the construction of a high quality consensus (Huang & Madan 1999). The clustering procedure can, therefore, have two outcomes: consensus are generated by the alignment of the sequences of a cluster and singlets result from sequences that have not been grouped to any others. Theoretically, each sequence (either a consensus or a singlet) should represent an individual gene, and so, these sequences are called uniques. As is expected that each sequence represents a single gene, the comparison of the number of uniques with the total number of predicted genes make it possible to know, approximately, how many genes have not been discovered yet.

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Gene regulation by Y-box proteins: coupling control of transcription and translation

Cited by 282 publications

References 51 publications

Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells

Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells

Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen

Clustering of Schistosoma mansoni mRNA sequences and analysis of the most transcribed genes: implications in metabolism and biology of different developmental stages

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