Gene Pyramiding and Multiple Character Breeding

Kushwah, Ashutosh; Gupta, Soma; Bindra, Shayla; Johal, Norah; Singh, Inderjit; Bharadwaj, Chellapilla; Dixit, G. P.; Gaur, Pooran M.; Nayyar, Harsh; Singh, Sarvjeet

doi:10.1016/b978-0-12-813522-8.00006-6

Cited by 33 publications

(28 citation statements)

References 115 publications

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“…Most crop breeding strategies for abiotic and biotic stress resistance are based on the insertion of a single resistant gene into plants, and thus crop resistance only lasts for a short period of time [23,24]. Therefore, the development of genotypes with resistance against several stresses by pyramiding multiple genes from different sources into a single plant is now emphasized [25][26][27][28]. Crop stress tolerance development has been elucidated in several studies by the pyramiding of multiple resistance genes [29,30] (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Gene Pyramiding for Sustainable Crop Improvement against Biotic and Abiotic Stresses

Dormatey¹,

Sun²,

Ali³

et al. 2020

Preprint

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Sustainable agricultural production is endangered by several ecological factors such as drought, extreme temperatures, excessive salts, parasitic ailments, and insect pest infestation. These challenging environmental factors may have adverse effects on future agriculture production in many countries. In modern agriculture, conventional crop breeding techniques alone are inadequate for achieving the increasing population’s food demand on a sustainable basis. The advancement of molecular genetics and related technologies are promising tools for the selection of new crop species. Gene pyramiding through marker assisted selection (MAS) and other techniques have accelerated the development of durable resistant/tolerant lines with high accuracy in the shortest possible period of time for agricultural sustainability. Gene stacking has not been fully utilized for biotic stress resistance development and quality improvement in most of the major cultivated crops. This review emphasizes on gene pyramiding techniques that are being successfully deployed in modern agriculture for improving crop tolerance to abiotic and biotic stresses for sustainable crop improvement.

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Section: Introductionmentioning

confidence: 99%

Gene Pyramiding for Sustainable Crop Improvement against Biotic and Abiotic Stresses

Dormatey¹,

Sun²,

Ali³

et al. 2020

Preprint

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“…These new methods have aided in the discovery of new, essential R-genes with ease and facilitated their combination into a single variety. The breakdown of pyramided genes has also been recorded in several experiments and explained the theoretical projection (Delmotte et al, 2016;Rana et al, 2019). In turn, this creates probabilities of the emergence of multi-virulent pathogen strains, such as M. oryzae.…”

Section: Gene Pyramiding For Blast Resistancementioning

confidence: 81%

Breeding Strategies and Challenges in the Improvement of Blast Disease Resistance in Finger Millet. A Current Review

Mbinda

Masaki

2021

Front. Plant Sci.

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Climate change has significantly altered the biodiversity of crop pests and pathogens, posing a major challenge to sustainable crop production. At the same time, with the increasing global population, there is growing pressure on plant breeders to secure the projected food demand by improving the prevailing yield of major food crops. Finger millet is an important cereal crop in southern Asia and eastern Africa, with excellent nutraceutical properties, long storage period, and a unique ability to grow under arid and semi-arid environmental conditions. Finger millet blast disease caused by the filamentous ascomycetous fungus Magnaporthe oryzae is the most devastating disease affecting the growth and yield of this crop in all its growing regions. The frequent breakdown of blast resistance because of the susceptibility to rapidly evolving virulent genes of the pathogen causes yield instability in all finger millet-growing areas. The deployment of novel and efficient strategies that provide dynamic and durable resistance against many biotypes of the pathogen and across a wide range of agro-ecological zones guarantees future sustainable production of finger millet. Here, we analyze the breeding strategies currently being used for improving resistance to disease and discuss potential future directions toward the development of new blast-resistant finger millet varieties, providing a comprehensive understanding of promising concepts for finger millet breeding. The review also includes empirical examples of how advanced molecular tools have been used in breeding durably blast-resistant cultivars. The techniques highlighted are cost-effective high-throughput methods that strongly reduce the generation cycle and accelerate both breeding and research programs, providing an alternative to conventional breeding methods for rapid introgression of disease resistance genes into favorable, susceptible cultivars. New information and knowledge gathered here will undoubtedly offer new insights into sustainable finger millet disease control and efficient optimization of the crop’s productivity.

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“…The general procedure of MAS is given in Figure 1 (Rana et al, 2019). Marker-assisted selection involves the following major methods: (1) screening of populations (e.g., F2, F3, recombinant inbred lines, double haploids, etc.)…”

Section: Procedures Of Masmentioning

confidence: 99%

“…References AFLP (Amplified fragment length polymorphism) used for DNA fingerprinting (Vuylsteke et al, 2007) AP-PCR (Arbitrarily primed PCR) used for genomic fingerprinting (Welsh & McClelland, 1991) AS-PCR (Allele-specific PCR) used for detection of mutations, polymorphisms, and haplotypes (Bottema et al, 1993) ASAP (Allele-specific Associated Primers) used for developing resistance in Pisum sativum against bean yellow mosaic virus (Yu et al, 1996) CAPS (Cleaved amplified polymorphic sequences) used for preparation of genetic map (Shavrukov, 2016) DAF (DNA amplification fingerprinting) used for producing a characteristic spectrum of short DNA products useful for detecting genetic differences (Caetano-Anollés et al, 1991) ISA (Inter-SSR amplification) used for genome fingerprinting (Zietkiewicz et al, 1994) RAPD (Random-amplified polymorphic DNA) used for comparing DNA sequences (Kumar & Gurusubramanian, 2011) RFLP (Restriction fragment length polymorphism) used to characterize the microbial communities (Schütte et al, 2008) SAP (Specific amplicon polymorphism) for analysis of PCR products amplified from mapped loci of rice genomic DNA (Williams et al, 1991) SCAR (Sequence characterized amplified region) used for Bdv2 gene's molecular confirmation in wheat germplasm and assessment for resistance against barely yellow dwarf viruses (Kausar et al, 2015) SPAR (Single Primer Amplification Reactions) for the assessment of diversity in Jatropha curcas L. (Ranade et al, 2008) SSLP (Microsatellite simple sequence length polymorphism) for its characterization in rice (Panaud et al, 1996) SSR (Simple sequence repeats) for analysis of its polymorphism between N22 and Uma rice varieties (Waghmare et al, 2018) STS (Sequence tagged sites) for its Generation and validation from diverse genotypes of dioecious Jojoba (Heikrujam et al, 2014) (Rana et al, 2019) index combining molecular and phenotypic data, and (6) genomic selection, in which genomic estimated breeding value is obtained using information from genome-wide markers.…”

Section: Techniquesmentioning

confidence: 99%

Marker-assisted selection: A smart biotechnological strategy for modern plant breeding

Shrestha

Subedi

Shrestha

2020

PJA

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Plant breeders and geneticists use molecular marker-assisted selection also called as MAS as a useful approach for breeding of plant to make selection more efficient and speed up the breeding cycle. MAS can be more efficient, effective, and reliable than phenotypic selection. Molecular markers are useful to identify the economically important traits in the breeding population for further manipulation in a short time. Due to the applicability of markers at the seedling stage ensuring high precision at the reduced level of cost, marker-assisted selection offer the chances to improve responses from selection. The MAS using DNA level polymorphism accelerate the pace of selection. The main marker technologies applied are chiefly co-dominant markers i.e. microsatellite markers/SSR (Simple Sequence Repeats) marker, RFLP (Restriction Fragment Length Polymorphism) marker and SNPs (Single nucleotide polymorphisms). This review overviews the various MAS technologies and their applications in crop improvement programs.

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Gene Pyramiding and Multiple Character Breeding

Cited by 33 publications

References 115 publications

Gene Pyramiding for Sustainable Crop Improvement against Biotic and Abiotic Stresses

Gene Pyramiding for Sustainable Crop Improvement against Biotic and Abiotic Stresses

Breeding Strategies and Challenges in the Improvement of Blast Disease Resistance in Finger Millet. A Current Review

Marker-assisted selection: A smart biotechnological strategy for modern plant breeding

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