Gene limiting cadmium accumulation in rice

Ueno, Daisei; Yamaji, Naoki; Kono, Izumi; Huang, Chao‐Feng; Ando, Tsuyu; Yano, Masahiro; Feng, Jian

doi:10.1073/pnas.1005396107

Cited by 691 publications

(716 citation statements)

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“…Among rice P-type ATPases characterized, OsHMA2 transports Zn and Cd (Satoh-Nagasawa et al, 2012;Takahashi et al, 2012;Yamaji et al, 2013), OsHMA3 is involved in Cd accumulation (Ueno et al, 2010;Miyadate et al, 2011), and OsHMA9 is responsible for the transport of Zn, Cu, Pb, and Cd (Lee et al, 2007). Knockout of OsHMA5 only resulted in altered Cu concentration, but not other metals, including Zn, Fe, and Mn (Fig.…”

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

“…et al, 2013), but OsHMA2 is mainly localized in the phloem region in node I (Yamaji et al, 2013), while OsHMA5 is localized in the xylem region. On the other hand, OsHMA3 is localized to the tonoplast of all root cells (Ueno et al, 2010), but OsHMA9 is localized to the plasma membrane in vascular bundles and anthers (Lee et al, 2007). The localization specificity of these HMA members may determine their physiological roles in metal transport in plants.…”

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

“…Furthermore, OsHMA2 at the node is required for preferential distribution of Zn to young leaves and panicles (Yamaji et al, 2013). OsHMA3 is localized to the tonoplast of the root cells and responsible for the sequestration of Cd into the vacuoles (Ueno et al, 2010;Miyadate et al, 2011). On the other hand, OsHMA9 was mainly expressed in vascular tissues, including the xylem and phloem (Lee et al, 2007).…”

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A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

et al. 2013

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ORCID ID: 0000-0003-3411-827X (J.F.M.).Heavy metal-transporting P-type ATPase (HMA) has been implicated in the transport of heavy metals in plants. Here, we report the function and role of an uncharacterized member of HMA, OsHMA5 in rice (Oryza sativa). Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage. At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type. The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice. OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage. The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage. Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk. Furthermore, immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane. Expression of OsHMA5 in a Cu transport-defective mutant yeast (Saccharomyces cerevisiae) strain restored the growth. Taken together, OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

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Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

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A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

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“…Recently, HMA3 has been cloned from Thlaspi carulescens Alpine Penny-cress (Ueno et al, 2011), and rice (Ueno et al, 2010), therefore it is a vacuolar influx transporter, important for cadmium tolerance in both species. This suggests that different homologs of HMA3 may have different metal-substrate specificity.…”

Section: Genes Involved In Zinc Homeostasis In Plantsmentioning

confidence: 99%

Strategies to increase zinc deficiency tolerance and homeostasis in plants

Henriques

Chalfun-Júnior

Aarts

2012

Braz. J. Plant Physiol.

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Zinc deficiency is a global problem of considerable importance for agriculture and human health. Under zinc deficiency conditions, many essential zinc-dependent physiological functions are unable to operate normally, and the cellular homeostasis is adversely affected. This paper described the potential damages that low-zinc bioavailability in soil can have for plants, humans, and animals. In addition, current knowledge on physiological and molecular aspects of zinc homeostasis in plants and strategies used to increase zinc deficiency tolerance were discussed.

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“…The past two decades have seen much discussion on the prospects of phytoremediation-the use of plants to remediate polluted soils (Cunningham and Ow, 1996;Salt et al, 1998;Singh et al, 2003). Likewise, much interest has been raised on the genetic improvement of crop varieties that could reduce dietary intake of these pollutants, as might be possible if toxic metals accumulate less in edible tissues (Ueno et al, 2010). Toward these aims, a better understanding of heavy metal allocation and detoxification in plants is of great importance.…”

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Fission Yeast HMT1 Lowers Seed Cadmium through Phytochelatin-Dependent Vacuolar Sequestration in Arabidopsis

et al. 2012

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Much of our dietary uptake of heavy metals is through the consumption of plants. A long-sought strategy to reduce chronic exposure to heavy metals is to develop plant varieties with reduced accumulation in edible tissues. Here, we describe that the fission yeast (Schizosaccharomyces pombe) phytochelatin (PC)-cadmium (Cd) transporter SpHMT1 produced in Arabidopsis (Arabidopsis thaliana) was localized to tonoplast, and enhanced tolerance to and accumulation of Cd 2+ , copper, arsenic, and zinc. The action of SpHMT1 requires PC substrates, and failed to confer Cd 2+ tolerance and accumulation when glutathione and PC synthesis was blocked by L-buthionine sulfoximine, or only PC synthesis is blocked in the cad1-3 mutant, which is deficient in PC synthase. SpHMT1 expression enhanced vacuolar Cd 2+ accumulation in wild-type Columbia-0, but not in cad1-3, where only approximately 35% of the Cd 2+ in protoplasts was localized in vacuoles, in contrast to the near 100% found in wild-type vacuoles and approximately 25% in those of cad2-1 that synthesizes very low amounts of glutathione and PCs. Interestingly, constitutive SpHMT1 expression delayed root-to-shoot metal transport, and root-targeted expression confirmed that roots can serve as a sink to reduce metal contents in shoots and seeds. These findings suggest that SpHMT1 function requires PCs in Arabidopsis, and it is feasible to promote food safety by engineering plants using SpHMT1 to decrease metal accumulation in edible tissues.

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Gene limiting cadmium accumulation in rice

Cited by 691 publications

References 29 publications

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

Strategies to increase zinc deficiency tolerance and homeostasis in plants

Fission Yeast HMT1 Lowers Seed Cadmium through Phytochelatin-Dependent Vacuolar Sequestration in Arabidopsis

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