Gene expression profiling of the peripheral blood mononuclear cells of offspring of one type 2 diabetic parent

Safi, Sher Zaman; Qvist, Rajes; Chinna, Karuthan; Ashraf, Muhammad Aqeel; Paramasivam, Darishiani; Ismail, I.A.

doi:10.1007/s13410-015-0369-1

Cited by 23 publications

(21 citation statements)

References 37 publications

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“…There has been continuing documentation of gene expression studies in different situations: among offspring who had one parent with type 2 diabetes [24], PPARG (Pro12Ala) polymorphisms [25], and variants of a TCF7L2 gene in the north east part of Uttar Pradesh [26]. An earlier editorial in the journal summarized aspects of such studies [27].…”

mentioning

confidence: 99%

Diabetes and data in many forms

Sridhar¹

2016

Int J Diabetes Dev Ctries

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mentioning

confidence: 99%

Diabetes and data in many forms

Sridhar¹

2016

Int J Diabetes Dev Ctries

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“…The FTIR spectrum (Surhio et al, 2014, Safi et al, 2015) of KPAsp (a), KPAsp/CMCTS semi-IPN (b) and KPAsp/CMCTS IPN (c) hydrogels are shown in Fig. 3.…”

Section: Resultsmentioning

confidence: 99%

One-step synthesis of interpenetrating network hydrogels: Environment sensitivities and drug delivery properties

et al. 2016

Saudi Journal of Biological Sciences

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A novel interpenetrating network hydrogel for drug controlled release, composed of modified poly(aspartic acid) (KPAsp) and carboxymethyl chitosan (CMCTS), was prepared in aqueous system. The surface morphology and composition of hydrogels were characterized by SEM and FTIR. The swelling properties of KPAsp, KPAsp/CMCTS semi-IPN and KPAsp/CMCTS IPN hydrogels were investigated and the swelling dynamics of the hydrogels was analyzed based on the Fickian equation. The pH, temperature and salt sensitivities of hydrogels were further studied, and the prepared hydrogels showed extremely sensitive properties to pH, temperature, the ionic salts kinds and concentration. The results of controlled drug release behaviors of the hydrogels revealed that the introduction of IPN observably improved the drug release properties of hydrogels, the release rate of drug from hydrogels can be controlled by the structure of the hydrogels and pH value of the external environment, a relative large amount of drug released was preferred under simulated intestinal fluid. These results illustrated high potential of the KPAsp/CMCTS IPN hydrogels for application as drug carriers.

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“…Diabetes mellitus (DM) is a serious global health issue. According to the World Health Organization, approximately 180 million people worldwide currently have type 2 DM, and this number is expected to double by 2030 [ 32 , 33 ]. Diabetes is a disease that is strongly associated with various complications including insulin resistance and pancreatic β-cell dysfunction [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Stimulation of β-adrenergic receptors plays a protective role via increased expression of RAF-1 and PDX-1 in hyperglycemic rat pancreatic islet (RIN-m5F) cells

Safi

Qvist

Ong

et al. 2017

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IntroductionIt is a widely held view that a progressive reduction of beta-cell mass occurs in the progression of diabetes. RAF-1 kinase and pancreas duodenal homeobox 1 (PDX-1) are major factors that promote survival of cells and maintain normal insulin functions. In this study we investigated the effect of a β-adrenergic receptor agonist and antagonist on RAF-1 and PDX-1, and their respective effects on apoptosis and insulin release in RIN-m5F cells.Material and methodsRIN-m5F cells were cultured in normal (5 mM) and high (25 mM) glucose to mimic diabetic conditions, followed by treatment with 5 µM, 10 µM and 20 µM of isoproterenol and isoproterenol + propranolol for 6, 12 and 24 h. Western blotting and reverse transcription analysis were performed to examine the expression of RAF-1 and PDX-1. Annexin-V-FITC and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assays were used to investigate apoptosis. ELISA was used to measure insulin levels. Reverse transcription polymerase chain reaction was conducted to investigate the expression of genes.ResultsStimulation of β-adrenergic receptors with isoproterenol significantly induced RAF-1 and PDX-1 genes in a concentration-dependent and time-independent manner. Changes were significant both at protein and mRNA levels. Up-regulation of RAF-1 and PDX-1 was accompanied by improved insulin levels and reduced apoptosis. Concentrations of 10 µM and 20 µM for 12 and 24 h were more effective in achieving significant differences in the experimental and control groups. Propranolol reversed the effect of isoproterenol mostly at maximum concentrations and time periods.ConclusionsA positive effect of a β-adrenergic agonist on RAF-1 and PDX-1, reduction in β-cell apoptosis and improved insulin contents can help to understand the pathogenesis of diabetes and to develop novel approaches for the β-cell dysfunction in diabetes.

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Gene expression profiling of the peripheral blood mononuclear cells of offspring of one type 2 diabetic parent

Cited by 23 publications

References 37 publications

Diabetes and data in many forms

Diabetes and data in many forms

One-step synthesis of interpenetrating network hydrogels: Environment sensitivities and drug delivery properties

Stimulation of β-adrenergic receptors plays a protective role via increased expression of RAF-1 and PDX-1 in hyperglycemic rat pancreatic islet (RIN-m5F) cells

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