Gene expression profile and physiological and biochemical characterization of hexaploid wheat inoculated with Blumeria graminis f. sp. tritici

Ma, Lu; Zhong, Shengyi; Liu, N.; Chen, W.Q.; Liu, T.G.; Li, X.; Zhang, M.; Ren, Zhihua; Yang, Jizhi; Luo, Peigao

doi:10.1016/j.pmpp.2015.02.005

Cited by 14 publications

(21 citation statements)

References 30 publications

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“…In addition to causing symptoms in the spike, FHB seriously affects the developing seeds. Since the developing seeds are the most important carbohydrate sinks, and there is a dynamic balance between carbohydrate sources (e.g., the leaves) and sinks [ 11 , 12 ], changes in gene expression in various tissues would be expected to occur upon plant pathogen attack [ 13 ]. Therefore, it would be interesting to characterize changes in gene expression in both the leaves and the spikes of wheat plants during FHB infection and to use the expression levels in the leaves as a control when considering gene expression changes in the spike.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Zhong

Chen

et al. 2018

IJMS

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Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is one of the most destructive fungal diseases of wheat (Triticum aestivum L.). Because of the quantitative nature of FHB resistance, its mechanism is poorly understood. We conducted a comparative transcriptome analysis to identify genes that are differentially expressed in FHB-resistant and FHB-susceptible wheat lines grown under field conditions for various periods after F. graminearum infection and determined the chromosomal distribution of the differentially expressed genes (DEGs). For each line, the expression in the spike (which exhibits symptoms in the infected plants) was compared with that in the flag leaves (which do not exhibit symptoms in the infected plants). We identified an island of 53 constitutive DEGs in a 140 kb region with high homology to the FhbL693b region on chromosome 3B. Of these genes, 13 were assigned to specific chloroplast-related pathways. Furthermore, one gene encoded inositol monophosphate (IMPa) and two genes encoded ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Our findings suggest that the temporary susceptibility in locally infected spikes results from the cross-talk between RuBisCO and IMPa, which blocks secondary signaling pathways mediated by salicylic acid and induces a systemic acquired resistance in the distant leaf tissue.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Zhong

Chen

et al. 2018

IJMS

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“…Two wheat lines, the powdery mildew resistant line PI 672538 [ 26 ] that carries the Pm40 gene without PmL962 [ 27 ] and the susceptible line L1034, were selected from the F 7 populations of a cross between the susceptible line MY11 and the resistant line YU25. Powdery mildew resistance in YU25 is putatively derived from Th .…”

Section: Methodsmentioning

confidence: 99%

“…From agronomical traits and application standpoints of view, we developed the following four spring wheat lines: L658 (PI 672537), L693 (PI 672538), L696 (PI 672539), and L699 (PI 672540). These lines exhibited excellent agronomic traits, including yield and resistance to Fusarium head blight conferred by FhbL693a and FhbL693b [ 24 ], to stripe rust conferred by YrL693 [ 25 ] and to powdery mildew conferred by Pm40 [ 22 , 23 , 26 , 27 ]. Though the pedigree of PI 672538 includes Th .…”

Section: Introductionmentioning

confidence: 99%

“…To accelerate the application of Pm40 in wheat breeding programs, we have explored the underlying molecular mechanism of resistance to powdery mildew as conferred by the host gene Pm40 , including studying the physiological changes and gene expression profile of Pm40 during host-pathogen interaction [ 27 ]. Although we have constructed genetic linkage maps of Pm40 , the exact gene location is still unclear, and gene-based markers, such as EST-STS markers, for molecular marker-assisted selection remain elusive.…”

Section: Introductionmentioning

confidence: 99%

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Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

Zhong

Fatima

et al. 2016

PLoS ONE

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The wheat powdery mildew resistance gene Pm40, which is located on chromosomal arm 7BS, is effective against nearly all prevalent races of Blumeria graminis f. sp tritici (Bgt) in China and is carried by the common wheat germplasm PI 672538. A set of the F1, F2 and F2:3 populations from the cross of the resistant PI 672538 with the susceptible line L1034 were used to conduct genetic analysis of powdery mildew resistance and construct a high-density linkage map of the Pm40 gene. We constructed a high-density linkage genetic map with a total length of 6.18 cM and average spacing between markers of 0.48 cM.Pm40 is flanked by Xwmc335 and BF291338 at genetic distances of 0.58 cM and 0.26 cM, respectively, in deletion bin C-7BS-1-0.27. Comparative genomic analysis based on EST-STS markers established a high level of collinearity of the Pm40 genomic region with a 1.09-Mbp genomic region on Brachypodium chromosome 3, a 1.16-Mbp genomic region on rice chromosome 8, and a 1.62-Mbp genomic region on sorghum chromosome 7. We further anchored the Pm40 target intervals to the wheat genome sequence. A putative linear index of 85 wheat contigs containing 97 genes on 7BS was constructed. In total, 9 genes could be considered as candidates for the resistances to powdery mildew in the target genomic regions, which encoded proteins that were involved in the plant defense and response to pathogen attack. These results will facilitate the development of new markers for map-based cloning and marker-assisted selection of Pm40 in wheat breeding programs.

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“…In China, powdery mildew is increasing its importance and for the last decade more than six million hectares of wheat cultivated area have been affected annually. Currently it is more dangerous than any other wheat diseases for this region (Zeng et al, 2014;Ma et al, 2015;Shen et al, 2015;Mwale et al, 2017). Some researchers predict powdery mildew to become more important in the future because of the climate change, which will provide more favourable conditions for the development of the disease (Tang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Virulence and diversity of the Blumeria graminis f. sp. tritici populations in Lithuania and Southern Ukraine

Traskovetskaya

Gorash

Liatukas

et al. 2019

Zemdirbyste-Agriculture

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For the present study, samples of cleistothecia were collected in Central Lithuania and Southern Ukraine. To characterize the virulence, complexity and diversity of powdery mildew (Blumeria graminis f. sp. tritici) populations, 80 isolates were derived from single ascospores, 40 isolates from each population. Pathotype analysis was conducted on 16 differentials with known Pm genes. According to the proposed nomenclature, 32 pathotypes were identified in the Lithuanian powdery mildew population and 30-in the Ukrainian population. The most frequent phenotype in Lithuania was NGDE (7.5%), and in Ukraine it was NGKE (15%). The Ukrainian powdery mildew population was more complex and contained more virulence genes per isolate. The virulence test was carried out by inoculation on detached leaves of 26 common wheat (Triticum aestivum L.) differentials with known Pm genes and 12 resistant winter wheat breeding lines and cultivars. The frequencies of virulence to these differentials ranged from 0% to 100%, and most of them had high level of virulence. No virulence was found to Pm20 and Pm25+3a genes, cultivar 'Lastivka odeska' and breeding lines CN 89/16 and PI 170911 in both populations, whereas the frequencies of virulence were more than 50% to Pm1a,

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Gene expression profile and physiological and biochemical characterization of hexaploid wheat inoculated with Blumeria graminis f. sp. tritici

Cited by 14 publications

References 30 publications

Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

Virulence and diversity of the Blumeria graminis f. sp. tritici populations in Lithuania and Southern Ukraine

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