Gene expression of Candida albicans strains isolates from patients with denture stomatitis submitted to treatments with photodynamic therapy and nystatin

Alonso, Gilberto; Klein, Marlise I.; Jordão, Cláudia Carolina; Carmello, Juliana Cabrini; Pavarina, Ana Cláudia

doi:10.1016/j.pdpdt.2021.102292

Cited by 6 publications

(22 citation statements)

References 56 publications

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“…is C. albicans. The data presented herein regarding genes are exclusively related to C. albicans [62][63][64][65][66]. Regarding the experimental models, Candida spp.…”

Section: Discussionmentioning

confidence: 99%

“…from oxidative stress. Alonso et al [66] sought to determine the expressions of the ALS1, HWP1, EFG1, CAP1, CAT1, SOD1, SAP1, PLB1 and LIP3 genes, which are virulence factors in C. albicans. The authors used both conventional nystatin therapy and aPDT to treat C. albicans isolates obtained from the palates and dentures of patients from a dental school in São Paulo, Brazil.…”

Section: Changes In Gene Expressionmentioning

confidence: 99%

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New Applications of Photodynamic Therapy in the Management of Candidiasis

Rodríguez-Cerdeira

Martínez‐Herrera

Fabbrocini

et al. 2021

JoF

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The most important aetiological agent of opportunistic mycoses worldwide is Candida spp. These yeasts can cause severe infections in the host, which may be fatal. Isolates of Candida albicans occur with greater frequency and variable resistance patterns. Photodynamic therapy (PDT) has been recognised as an alternative treatment to kill pathogenic microorganisms. PDT utilises a photosensitizer, which is activated at a specific wavelength and oxygen concentration. Their reaction yields reactive oxygen species that kill the infectious microorganism. A systematic review of new applications of PDT in the management of candidiasis was performed. Of the 222 studies selected for in-depth screening, 84 were included in this study. All the studies reported the antifungal effectiveness, toxicity and dosimetry of treatment with antimicrobial PDT (aPDT) with different photosensitizers against Candida spp. The manuscripts that are discussed reveal the breadth of the new applications of aPDT against Candida spp., which are resistant to common antifungals. aPDT has superior performance compared to conventional antifungal therapies. With further studies, aPDT should prove valuable in daily clinical practice.

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“…is C. albicans. The data presented herein regarding genes are exclusively related to C. albicans [62][63][64][65][66]. Regarding the experimental models, Candida spp.…”

Section: Discussionmentioning

confidence: 99%

Section: Changes In Gene Expressionmentioning

confidence: 99%

New Applications of Photodynamic Therapy in the Management of Candidiasis

Rodríguez-Cerdeira

Martínez‐Herrera

Fabbrocini

et al. 2021

JoF

View full text Add to dashboard Cite

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“…The number of subjects in each included study ranged between 22 and 65. Five studies [14,15,22,26,35] were conducted in Brazil, four [23,25,27,31] in Saudi Arabia, one [24] in India, and one in Iran [34]. Only eight studies [14,15,23,[25][26][27]31] reported gender of the participants where the majority were females.…”

Section: General Characteristicsmentioning

confidence: 99%

“…It is worth mentioning, however, that the aforementioned reviews included only very limited number of studies (3-5 studies) and failed to include all potentially eligible studies, and thus the results might be inconclusive. Additionally, since then more recent relevant clinical trials assessing the e cacy of PDT in DS have been published [22,27,31], again with interesting results.…”

Section: Introductionmentioning

confidence: 96%

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Efficacy of photodynamic therapy on candida colonization and clinical symptoms in denture stomatitis: An updated systematic review and meta-analysis

Al-Maweri,

Alhajj,

Anweigi

et al. 2023

Preprint

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Background: Photodynamic therapy (PDT) has recently been proposed as a promising therapeutic option for Denture Stomatitis (DS). This updated systematic review and meta-analysis investigated the current evidence regarding the efficacy of PDT for DS. Materials and methods: PubMed, Scopus, Web of Science, Google Scholar, and ProQuest were searched up to June 7, 2023. All relevant clinical trials were included. RevMan software was used for meta-analysis. Results: Elven randomized clinical trials (460 DS patients) were included. Eight studies assessed the efficacy of PDT vs. topical antifungal, while three studies assessed the adjunctive use of PDT (PDT+antifungal therapy) vs. topical antifungal therapy alone. The results revealed insignificant less efficacy of PDT in comparison to topical antifungal therapy on candida colonization at 15 days (MD: 0.95, 95% CI: -0.28, 2.19, p = 0.13), but comparable results at the end of follow-up (MD: -0.17, 95% CI: -1.33, 0.98, p = 0.77). The pooled two studies revealed relative better efficacy of adjunctive use of PDT with antifungal therapy on candida colonization compared to antifungal therapy alone at 15 days (MD: -6.67, 95% CI: -15.15, 1.82, p = 0.12), and at the end of follow-up (MD: -7.14, 95% CI: -19.78, 5.50, p = 0.27). Additionally, the results revealed comparable efficacy of PDT and topical antifungal therapy in improvement of the clinical signs. Conclusions: PDT might be a viable option for DS as an adjunct or an alternative to the topical antifungal medications. Further studies with large sample sizes and standardized PDT parameters are warranted.

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An Electroluminodynamic Flexible Device for Highly Efficient Eradication of Drug‐Resistant Bacteria

et al. 2022

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treatments. [2] Especially, compared with conventional chemotherapy, PDT acts its biocidal activity mainly through the oxidative damage of biological macromolecules such as phospholipids, enzymes, proteins, and DNA in pathological tissue/cells, and therefore is difficult to induce drug resistance. [3] The drastic emergence of antibiotic resistance has evolved into a worldwide health crisis, and PDT is considered as a promising technology to combat drugresistant bacteria. [4] Although it shows strong curative antibacterial effect, PDT usually needs to be driven by external physical light, which makes it available in special environment or assisted by bulky equipment. [5] Therefore, the development of more convenient, universal, and manipulatable driving PDT strategies is highly desired.Recently, several attempts have been made to use in situ self-luminescence, such as bioluminescence, electrochemiluminescence (ECL), and chemiluminescence (CL), as excitation light source for driving PDT. [6] For example, a bioluminescent luciferase conjugated quantum dot was prepared, which could emit light and excite commercial photosensitizer micelles (Foscan) while interacting with coelenterazine, and successfully generated ROS and killed ≈50% lung cancer cells. [7] In 2018, Wang et al. exploited an electric-driven device to emit ECL by electrochemical reaction of luminescent agent (luminol) and H 2 O 2 , which further excited their synthesized polythiophene photosensitizer to produce ROS, and achieved 67-79% sterilization of bacteria and yeast. [8] More recently, Fan and co-workers also prepared a penetration-independent nano-photosensitizer Photodynamic therapy (PDT) has attracted wide attention in antibacterial applications due to its advantages of spatial-temporal selectivity, noninvasiveness, and low incidence to develop drug resistance. To make it more convenient, universal, and manipulatable for clinical application, a conceptually antibacterial strategy, namely "electroluminodynamic therapy" (ELDT), is presented by nanoassembly of an electroluminescent (EL) material and a photosensitizer, which is capable of generating reactive oxygen species (ROS) in situ under an electric field, i.e., the fluorescence emitted by the EL molecules excites the photosensitizer to generate singlet oxygen ( 1 O 2 ), for the oxidative damage of pathogens. Based on the scheme of ELDT, a flexible therapeutic device is fabricated through a hydrogel loading with ELDT nanoagents, followed by integration with a flexible battery, satisfying the requirements of being light and wearable for wound dressings. The ELDT-based flexible device presents potent ROS-induced killing efficacies against drug-resistant bacteria (>99.9%), so as to effectively inhibit the superficial infection and promote the wound healing. This research reveals a proof-of-concept ELDT strategy as a prospective alternative to PDT, which avoids the utilization of a physical light source, and achieves convenient and effective killing of drug-resistant bacteria through a hydrogel-ba...

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Gene expression of Candida albicans strains isolates from patients with denture stomatitis submitted to treatments with photodynamic therapy and nystatin

Cited by 6 publications

References 56 publications

New Applications of Photodynamic Therapy in the Management of Candidiasis

New Applications of Photodynamic Therapy in the Management of Candidiasis

Efficacy of photodynamic therapy on candida colonization and clinical symptoms in denture stomatitis: An updated systematic review and meta-analysis

An Electroluminodynamic Flexible Device for Highly Efficient Eradication of Drug‐Resistant Bacteria

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