Gene expression levels in some candidate genes for mastitis resistance, milk yield,and milk quality of goats reared under different feeding systems

Yakan, Akın; Özkan, Hüseyin; Şakar, Aysel Eraslan; Ünal, Necmettin; Özbeyaz, Ceyhan

doi:10.3906/vet-1704-7

Cited by 12 publications

(11 citation statements)

References 39 publications

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“…In agreement with present finding, butterfat, protein, lactose and energy values were significantly tended to rise with advancing lactation as reported in West African dwarf goats (Charnobai et al, 1999), whereas Zeng et al (1997) reported high fat, protein, solids non-fat and total solids with the exception of lactose, in the first month after parturition, which declined slightly and then remained constant until drying-off in Alpine does. In accordance with the present study, daily milk production increased steadily for the first 4 weeks following parturition and then decreased gradually as reported by Zeng et al (1997), however, Yakan et al (2018) reported increased daily milk yield (g) from 1 st month to 3 rd month of lactation in Damascus goats.…”

Section: Results a N D Discussionsupporting

confidence: 93%

“…SCC shows an upward trend with the progression of the productive period resulting in a significant negative correlation between SCC and milk production (Gomes et al, 2006). Significantly higher SCC was reported by Zeng et al (1997) in Alpine does during the first 2 weeks of lactation (887 ± 400 X 10 3 /ml) and Damascus goats from 1 st month (488.04 ± 107.86) to 3 rd month (730.08 ± 189.23 ×1000/ml) as reported by Yakan et al (2018).…”

Section: Results a N D Discussionmentioning

confidence: 83%

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Relative Gene Expression Study on Casein Protein and its Regulatory Genes in Mammary Epithelial Cells of Surti Goat

et al. 2020

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This investigation was carried out to study the relative gene expression of casein proteins and its regulatory genes in mammary epithelial cells of Surti goats at 30 and 90 days interval postpartum. 10 healthy Surti goats were selected from Livestock Research Station, Navsari. The non-invasive method was used for the isolation of Mammary Epithelial Cells (MEC) from goat milk samples. The relative gene expression of CSN1S1 (αs1-casein), CSN1S2 (αs2-casein), CSN2 (β-casein), CSN3 (κ-casein) and its regulatory genes C/EBP and STAT5A genes and KRT14 (epithelial cell marker) gene were studied in MEC. The relative gene expression of CSN1S1, CSN1S2, CSN3 and C/EBP genes were significantly up-regulated with the advancement of lactation at 90 days postpartum. The relative gene expression of CSN2 was highly significant and had a positive correlation with its regulatory genes C/EBP and STAT5A at 30 days and 90 days, respectively. The relative gene expression of CSN1S2, CSN3, and C/EBP genes was positively correlated with protein percent at 30 days and 90 days postpartum in Surti goats.

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Section: Results a N D Discussionsupporting

confidence: 93%

Section: Results a N D Discussionmentioning

confidence: 83%

Relative Gene Expression Study on Casein Protein and its Regulatory Genes in Mammary Epithelial Cells of Surti Goat

et al. 2020

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“…: Product Length; FASN: Fatty Acids Synthase; SCD: Stearoyl-CoA Desaturase; ACACA: Acetyl-CoA Carboxylase Alpha; COX-2: Cyclooxygenase-2; TLR2: Toll-like Receptor 2; NF-kB: Nuclear factor Kappa B; LTF: Lactoferrin; PTX3: Pentraxin 3; NRF2: Nuclear Factor Erythroid 2-related Factor 2; ACTB: Actin-beta; G6PD: Glucose-6-phosphate Dehydrogenase. Genes Accession no Forward and reverse sequences of primers P.L Ref FASN NM_001285629.1 F: 5′-GCACACAATATGGACCCCCA-3′ R: 5′-CATGCTGTAGCCTACGAGGG-3′ 183 * SCD NM_001285619.1 F: 5′-ATCGCCCTTACGACAAGACC-3′ R: 5′-CATAAGCCAGACCGATGGCA-3′ 186 * ACACA XM_018064168.1 F: 5′-GCCTGCCCGAGTTTTGAGTG-3′ R: 5′-CGCACTCTGGAGCGGATAAA-3′ 105 * COX-2 XM_018060731.1 F: 5′-GTAGGCCAGGAGGTCTTTGG-3′ R: 5′- GCCTGCTTGTCTGGAACAAC-3′ 142 * NRF2 NM_001314327.1 F: 5′-CTGTTCTCTGCTGTCAAGGG-3’ R: 5′-AACTCGCCGGTCTCTTCATC-3’ 222 * TLR2 NM_001285603.1 F: 5′-TGCTGTGCCCTCTTCCTGTT-3′ R: 5′-GGGACGAAGTCTCGCTTATGAA-3′ 260 47 NF-kB XM_018049265.1 F: 5′-TGGGGATACTGAACAACGCC-3′ R: 5′-ATCTGTCTCAGGGCCTCCAT-3′ 115 * LTF NM_001285548.1 F: 5′-CAAGTGTGTGCCCAACTCTA-3′ R: 5′-GCTCTCTCCATTCGTGTTCTC-3′ 105 19 PTX3 XM_018048475.1 …”

Section: Methodsmentioning

confidence: 99%

Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats

Yakan

Özkan

Çamdeviren

et al. 2021

Sci Rep

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The molecular regulation of milk secretion and quality in the transition period from colostrum to milk in goats is largely unknown. In the present study, mammary gland secretion of goats was collected in 0th, 4th, 7th, 14th and 28th days after parturition. In addition to composition and fatty acid profile of colostrum or milk, FASN, SCD, ACACA, COX-2, NRF2, TLR2, NF-kB, LTF and PTX3 genes expression patterns were determined from milk somatic cells. While somatic cell count (SCC), malondialdehyde (MDA), fat, fat-free dry matter, protein and lactose were highest as expression levels of the oxidative and inflammatory genes, freezing point and electrical conductivity were lowest in colostrum. With the continuation of lactation, most of the fatty acids, n3 ratio, and odour index increased but C14:0 and C16:0 decreased. While FASN was upregulated almost threefolds in 14th day, ACACA was upregulated more than fivefolds in 7th and 14th days. Separately, the major genes in fatty acid synthesis, inflammation and oxidative stress were significantly associated with each other due to being positively correlated. MDA was positively correlated with SCC and some of the genes related inflammation and oxidative stress. Furthermore, significant negative correlations were determined between SCC and fatty acid synthesis related genes. With this study, transition period of mammary secretion was particularly clarified at the molecular levels in Damascus goats.

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“…Unlike GECs, SCs are easily extracted from milk samples in forms of pellets. They were also effectively utilized to assess the expression of some milk performance-related genes and proteins in buffalo and goat [ 14 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes

El-Magd

Fathy

Kahilo

et al. 2021

Animals

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Prolactin (PRL) and its receptor (PRLR) were considered as potential genetic markers for milk production and quality traits in cattle. However, little information is available regarding PRLR genetic diversity and association studies with milk traits in Egyptian water buffaloes. Therefore, the present study was conducted to search for mutations in PRLR and determine their associations with milk performance in these animals. Exon3 (E3) and E10 of PRLR were screened for polymorphisms using single strand conformation polymorphism (SSCP) and sequencing in 400 buffaloes. The associations between haplotypes and milk production (fat%, protein%, lactose%, and solid%) traits as well as mRNA and protein levels of PRL and PRLR were studied. Two single nucleotide polymorphisms (SNPs) in E10 were detected: g.11685G>A (p.Ala494Thr) and g.11773T>C (p.Val523Aal). The G and T alleles were wild (ancestral) alleles, while the A and C alleles were mutant alleles. These SNPs resulted in four haplotypes; AC, AT, GC, and GT. Buffaloes with wild GT haplotypes showed significantly higher milk yield, fat% and protein%, mRNA and protein levels of PRL and PRLR in milk somatic cells than other animals. Animals carrying mutant AC haplotype had inferior milk traits and lowest levels of associated mRNAs and proteins. With these results, we could conclude that the selection of buffaloes with wild GT haplotypes for g.11685G>A and g.11773T>C SNPs of the PRLR gene might improve the milk production traits of Egyptian water buffaloes.

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Gene expression levels in some candidate genes for mastitis resistance, milk yield,and milk quality of goats reared under different feeding systems

Cited by 12 publications

References 39 publications

Relative Gene Expression Study on Casein Protein and its Regulatory Genes in Mammary Epithelial Cells of Surti Goat

Relative Gene Expression Study on Casein Protein and its Regulatory Genes in Mammary Epithelial Cells of Surti Goat

Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats

Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes

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