Gene Expression in Donor Corneal Endothelium

Specific expression of annexin A8 in adult murine stratified epithelia

“…The preferred location in the suprabasal layers in these murine stratified epithelia may hint at ANXA8 being involved in epithelial differentiation. The only simple epithelium, in which we detected ANXA8 immunohistochemically, was the cornea endothelium confirming previous reports on annexin A8 expression in this tissue (Gottsch et al 2003).…”

Specific expression of annexin A8 in adult murine stratified epithelia

“…The preferred location in the suprabasal layers in these murine stratified epithelia may hint at ANXA8 being involved in epithelial differentiation. The only simple epithelium, in which we detected ANXA8 immunohistochemically, was the cornea endothelium confirming previous reports on annexin A8 expression in this tissue (Gottsch et al 2003).…”

Postnatal Gene Expression in the Normal Mouse Cornea by SAGE

“…Notably, 758 tags from the two libraries had no match in any public database, and therefore represent novel genes. Similar to the SAGE of human normal 33 and Fuch's dystrophy corneal endothelium, 34 the expression profiles from this study reveal a characteristic fingerprint of the mouse cornea.…”

Efficient Generation of Human Embryonic Stem Cell-Derived Corneal Endothelial Cells by Directed Differentiation

“…Both Na + K + ATPase α 1 and Na + K + ATPase α 3 mRNA were highly expressed by hESC-CECs, however, naïve hESCs also expressed Na + /K + ATPase α 1 and Na + /K + ATPase α 3, which made these two not useful as differentiation markers (data not shown). Similarly, other corneal endothelial pump function genes SLC16A1, SLC9A1, SLC4A2, ADCY10, and CA12 [ 42 – 45 ] were expressed by hESC-CECs, but were not upregulated compared to hESCs (data not shown). On the other hand, aquaporin 1, another vital pump component [ 46 ], was expressed by hESC-CECs in increasing levels with time, indicating a maturation of the hESC-CEC ( Fig 5D ).…”

Specific expression of annexin A8 in adult murine stratified epithelia

“…The preferred location in the suprabasal layers in these murine stratified epithelia may hint at ANXA8 being involved in epithelial differentiation. The only simple epithelium, in which we detected ANXA8 immunohistochemically, was the cornea endothelium confirming previous reports on annexin A8 expression in this tissue (Gottsch et al 2003).…”

Postnatal Gene Expression in the Normal Mouse Cornea by SAGE

“…Notably, 758 tags from the two libraries had no match in any public database, and therefore represent novel genes. Similar to the SAGE of human normal 33 and Fuch's dystrophy corneal endothelium, 34 the expression profiles from this study reveal a characteristic fingerprint of the mouse cornea.…”

Efficient Generation of Human Embryonic Stem Cell-Derived Corneal Endothelial Cells by Directed Differentiation

“…Both Na + K + ATPase α 1 and Na + K + ATPase α 3 mRNA were highly expressed by hESC-CECs, however, naïve hESCs also expressed Na + /K + ATPase α 1 and Na + /K + ATPase α 3, which made these two not useful as differentiation markers (data not shown). Similarly, other corneal endothelial pump function genes SLC16A1, SLC9A1, SLC4A2, ADCY10, and CA12 [ 42 – 45 ] were expressed by hESC-CECs, but were not upregulated compared to hESCs (data not shown). On the other hand, aquaporin 1, another vital pump component [ 46 ], was expressed by hESC-CECs in increasing levels with time, indicating a maturation of the hESC-CEC ( Fig 5D ).…”

Specific expression of annexin A8 in adult murine stratified epithelia

“…The preferred location in the suprabasal layers in these murine stratified epithelia may hint at ANXA8 being involved in epithelial differentiation. The only simple epithelium, in which we detected ANXA8 immunohistochemically, was the cornea endothelium confirming previous reports on annexin A8 expression in this tissue (Gottsch et al 2003).…”

Postnatal Gene Expression in the Normal Mouse Cornea by SAGE

“…Notably, 758 tags from the two libraries had no match in any public database, and therefore represent novel genes. Similar to the SAGE of human normal 33 and Fuch's dystrophy corneal endothelium, 34 the expression profiles from this study reveal a characteristic fingerprint of the mouse cornea.…”

Efficient Generation of Human Embryonic Stem Cell-Derived Corneal Endothelial Cells by Directed Differentiation

“…Both Na + K + ATPase α 1 and Na + K + ATPase α 3 mRNA were highly expressed by hESC-CECs, however, naïve hESCs also expressed Na + /K + ATPase α 1 and Na + /K + ATPase α 3, which made these two not useful as differentiation markers (data not shown). Similarly, other corneal endothelial pump function genes SLC16A1, SLC9A1, SLC4A2, ADCY10, and CA12 [ 42 – 45 ] were expressed by hESC-CECs, but were not upregulated compared to hESCs (data not shown). On the other hand, aquaporin 1, another vital pump component [ 46 ], was expressed by hESC-CECs in increasing levels with time, indicating a maturation of the hESC-CEC ( Fig 5D ).…”