Gene expression in acute Stanford type A dissection: a comparative microarray study

Weis-Müller, Barbara Theresia; Modlich, Olga; Drobinskaya, Irina; Unay, Derya; Huber, Rita; Bojar, Hans; Schipke, Jochen D.; Feindt, P.; Gams, E.; Müller, Wolfram; Goecke, Timm O.; Sandmann, W.

doi:10.1186/1479-5876-4-29

Cited by 48 publications

(31 citation statements)

References 38 publications

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“…The previous microarray study also observed that IL-2, -6 and -8 genes were up-regulated in aortic dissection [15]. It has also been noted that IL-6 is secreted at high levels in human aortic aneurysm disease [20], [21].…”

Section: Discussionmentioning

confidence: 75%

JAK2-Centered Interactome Hotspot Identified by an Integrative Network Algorithm in Acute Stanford Type A Aortic Dissection

Sun

Teschendorff

et al. 2014

PLoS ONE

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The precise mechanisms underlying dissections, especially those without connective tissue diseases or congenital vascular diseases, are incompletely understood. This study attempted to identify both the expression profile of the dissected ascending aorta and the interactome hotspots associated with the disease, using microarray technology and gene regulatory network analysis. There were 2,737 genes differentially expressed between patients with acute Stanford type A aortic dissection and controls. Eight interactome hotspots significantly associated with aortic dissection were identified by an integrative network algorithm. In particular, we identified a JAK2-centered expression module, which was validated in an independent gene expression microarray data set, and which was characterized by over-expressed cytokines and receptors in acute aortic dissection cases, indicating that JAK2 may play a key role in the inflammatory process, which potentially contributes to the occurrence of acute aortic dissection. Overall, the analytical strategy used in this study offered the possibility to identify functional relevant network modules and subsequently facilitated the biological interpretation in the complicated disease.

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Section: Discussionmentioning

confidence: 75%

JAK2-Centered Interactome Hotspot Identified by an Integrative Network Algorithm in Acute Stanford Type A Aortic Dissection

Sun

Teschendorff

et al. 2014

PLoS ONE

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“…MMP-19 was upregulated and MMP-12 and MMP-9 were downregulated. Weis-Müller and colleagues [28] also compared tissue from dissected aorta with normal aortic tissue using microarrays andcould not detect MMP-12 expression in the array, but observed a higher gene expression of the matrix metalloproteinases MMP-11, MMP-14 and MMP-19. A possible explanation is that our gene expression studies considered the entry site of the dissection and not the whole aortic wall .…”

Section: Discussionmentioning

confidence: 99%

Expression of matrix metalloproteinase-12 in aortic dissection

Song

Xie

Liu

et al. 2013

BMC Cardiovasc Disord

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BackgroundAortic dissection(AD) is an acute process of large blood vessels characterized by dangerous pathogenic conditions and high disability and high mortality. The pathogenesis of AD remains debated. Matrix metalloproteinase-12 (MMP-12) participates in many pathological processes such as abdominal aortic aneurysm, atherosclerosis, emphysema and cancer. However, this elastase has rarely been assessed in the presence of AD. The aim of the present study was to investigate the expression of MMP-12 in aortic tissue so as to offer a better understanding of the possible mechanisms of AD.MethodsThe protein expression levels of MMP-12 were analyzed and compared in aorta tissue and the blood serum samples by reverse transcription polymerase chain reaction(RT-PCR), Western blotting, immuno-histochemistry, fluorescence resonance energy transfer ( FRET ) activity assay and enzyme-linked immuno sorbent assay ( ELISA ), respectively. Ascending aorta tissue specimens were obtained from 12 patients with an acute Stanford A-dissection at the time of aortic replacement, and from 4 patients with coronary artery disease (CAD) undergoing coronary artery bypass surgery. Meanwhile, serum samples were harvested from 15 patients with an acute Stanford A-dissection and 10 healthy individuals who served as the control group.ResultsMMP-12 activity could be detected in both AD and CAD groups, but the level in the AD group was higher than those in the CAD group (P < 0.05). MMP-12 proteolysis existed in both serum samples of the AD and healthy groups, and the activity level in the AD group was clearly higher than in the healthy group (P < 0.05). For AD patients, MMP-12 activity in serum was higher than in the aorta wall (P < 0.05). MMP-12 activity in the aortic wall tissue can be inhibited by MMP inhibitor v (P < 0.05).ConclusionThe present study directly demonstrates that MMP-12 proteolytic activity exists within the aorta specimens and blood samples from aortic dissection patients. MMP-12 might be of potential relevance as a clinically diagnostic tool and therapeutic target in vascular injury and repair.

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“…They compared tissue from dissected aorta with normal aortic tissue using microarrays and found some indications of AAD differentially expressed genes;19 however, the pathway of these differentially expressed genes is still to be defined. Also, the underlying mechanisms which make the aorta more prone to be dissected remain unclarified.…”

Section: Discussionmentioning

confidence: 99%

Pathway analysis of differentially expressed genes in patients with acute aortic dissection

Mohamed¹,

Hanke²,

Erasmi³

et al. 2007

Thorac cardiovasc Surg

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Background: Acute aortic dissection (AAD) is a life-threatening condition with high mortality and a relatively unclarifi ed pathophysiological mechanism. Although differentially expressed genes in AAD have been recognized, interactions between these genes remain poorly defi ned. This study was conducted to gain a better understanding of the molecular mechanisms underlying AAD and to support the future development of a clinical test for monitoring patients at high risk.

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Gene expression in acute Stanford type A dissection: a comparative microarray study

Abstract: Background: We compared gene expression profiles in acutely dissected aorta with those in normal control aorta.

Cited by 48 publications

References 38 publications

JAK2-Centered Interactome Hotspot Identified by an Integrative Network Algorithm in Acute Stanford Type A Aortic Dissection

JAK2-Centered Interactome Hotspot Identified by an Integrative Network Algorithm in Acute Stanford Type A Aortic Dissection

Expression of matrix metalloproteinase-12 in aortic dissection

Pathway analysis of differentially expressed genes in patients with acute aortic dissection

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