Gene Expression and Microarray Investigation of Dendrobium Mixture as Progressive Therapy for the Treatment of Type 2 Diabetes Mellitus

Xu, Qian; Liu, Yun; Yu, Cong; Zheng, Yuan-Yan; Zhang, Jieping; Cui, Yi; Shi, Hong

doi:10.4314/tjpr.v12i2.10

Cited by 6 publications

(8 citation statements)

References 13 publications

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“…The present study used a high-sugar and high-fat feed combined classic model of STZ-induced DN to successfully reproduce the typical pathological changes of DN in rats. The biochemical indexes of these rats were abnormal to varying degrees, and these were significantly improved after DMix treatment, which was consistent with the findings of our previous studies (21,22). Furthermore, the current study demonstrated the therapeutic effects of DMix in protecting renal function and preventing renal fibrosis.…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, any active ingredient of TCM alone cannot reflect the final therapeutic effect of the whole medicine (33,34). In preliminary experimental studies and clinical practice, DMix has been proved to have a good therapeutic effect against diabetes and its complications (21,22).…”

Section: Discussionmentioning

confidence: 99%

“…no. ZL201110408411.0), which was developed by Professor Hong Shi for the long-term clinical treatment of diabetes and its complications (21,22). DMix is composed of Dendrobium, Astragalus, Schisandra, Radix puerariae, Salvia miltiorrhiza, Rehmanniae and Rhizoma anemarrhenae.…”

Section: Introductionmentioning

confidence: 99%

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Dendrobium mixture attenuates renal damage in rats with diabetic nephropathy by inhibiting the PI3K/Akt/mTOR pathway

et al. 2021

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notable glomerular hypertrophy, an increase in mesangial matrix content and renal interstitial fibrosis. Moreover, DMix notably reduced kidney damage. The results demonstrated that DMix inhibited the phosphorylation of PI3K, Akt and mTOR in the kidney tissues of rats with DN, and increased the protein and mRNA expression levels of LC3 and Beclin-1. Therefore, it was suggested that DMix has protective effects on the kidney of rats with DN, which may be associated with the inhibition of the PI3K/Akt/mTOR signaling pathway and activation of renal autophagy by this traditional medicine.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Dendrobium mixture attenuates renal damage in rats with diabetic nephropathy by inhibiting the PI3K/Akt/mTOR pathway

et al. 2021

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“…Based on its clinical manifestations and features, diabetes is categorized as a dispersion-thirst disease in TCM ( 24 ). The incidence of diabetes is multifactorial and may be associated with genetic ( 25 ), environmental ( 26 ), dietary ( 27 ), lifestyle ( 27 ) factors. In TCM, type II diabetes may be treated with the Nourishing-‘yin’, Tonifying-‘qi’ method ( 28 ) or the Nourishing-‘yin’, Tonifying-qi, activating-blood (promoting blood flow) methods ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide‑3‑kinase/protein kinase B signaling pathway

Shi

Cui

et al. 2018

Exp Ther Med

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The present study aimed to evaluate the impact of dendrobium mixture (DMix) on the gene and protein expression of insulin signaling pathway-associated factors in the livers of diabetic rats. The molecular mechanisms by which DMix inhibits gluconeogenesis were also investigated. A total of 47 female Wistar rats were used in the present study. Of these, 11 rats were randomly selected as healthy controls and diabetes was induced in the remaining 36 rats by administering a high-fat and high-sugar diet for 6 weeks, followed by two intraperitoneal injections of streptozotocin. The 36 rats were screened for diabetes and then randomly divided into three groups: Model, metformin and DMix groups. Following 12 weeks of treatment, the fasting blood glucose (FBG), glycosylated serum protein (GSP), serum insulin, blood lipids [total cholesterol (Tch) and triglycerides (TG)], alanine transaminase (ALT) and aspartate transaminase (AST) were assessed. In addition, hematoxylin and eosin staining was used for histomorphological examination of the liver tissues. The mRNA expression of insulin receptor (InsR), forkhead box protein O1 (FoxO1), phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver was measured with reverse transcription-quantitative polymerase chain reaction and the protein expression of InsR, phosphoinositide-3-kinase (PI3K), phosphorylated (p)-PI3K, protein kinase B (Akt), p-Akt, FoxO1, PEPCK and G6Pase in the liver was measured by western blot analysis. The FBG, GSP, InsR, Tch, TG, ALT and AST levels were significantly lower in the DMix-treated group compared with the model group (P<0.05). In addition, DMix treatment notably improved liver histopathology and significantly increased the gene and protein expression of InsR, PI3K and Akt (P<0.05). DMix treatment also significantly reduced the gene and protein expression of FoxO1, PEPCK and G6Pase (P<0.05). DMix effectively reduced FBG and blood lipids and significantly improved liver function and insulin resistance in diabetic rats, possibly by regulating the gene and protein expression of molecules associated with the PI3K/Akt signaling pathway.

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“…Recently, Xu et al (2013) presented a theoretical basis for therapy of type-2 diabetes by searching for potential drug targets in the genome, using gene chip, also called cDNA microarray investigation, which refers to the many specific gene fragments fixed to a solid support [ 71 ]. The gene expression profile, as well as blood sugar lowering and lipid-lowering molecular mechanisms of Dendrobium mixtures in diabetic rat model were examined.…”

Section: Anti-diabetic Propertiesmentioning

confidence: 99%

Dendrobium: Sources of Active Ingredients to Treat Age-Related Pathologies

Čáková

Bonté²,

Lobstein

2017

Aging and disease

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Dendrobium represents one of the most important orchid genera, ornamentally and medicinally. Dendrobiums are sympodial epiphytic plants, which is a name they are worthy of, the name coming from Greek origin: "dendros", tree, and "bios", life. Dendrobium species have been used for a thousand years as first-rate herbs in traditional Chinese medicine (TCM). They are source of tonic, astringent, analgesic, antipyretic, and anti-inflammatory substances, and have been traditionally used as medicinal herbs in the treatment of a variety of disorders, such as, nourishing the stomach, enhancing production of body fluids or nourishing Yin. The Chinese consider Dendrobium as one of the fifty fundamental herbs used to treat all kinds of ailments and use Dendrobium tonic for longevity. This review is focused on main research conducted during the last decade (2006-2016) on Dendrobium plants and their constituents, which have been subjected to investigations of their pharmacological effects involving anticancer, anti-diabetic, neuroprotective and immunomodulating activities, to report their undeniable potential for treating age-related pathologies.

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Gene Expression and Microarray Investigation of Dendrobium Mixture as Progressive Therapy for the Treatment of Type 2 Diabetes Mellitus

Cited by 6 publications

References 13 publications

Dendrobium mixture attenuates renal damage in rats with diabetic nephropathy by inhibiting the PI3K/Akt/mTOR pathway

Dendrobium mixture attenuates renal damage in rats with diabetic nephropathy by inhibiting the PI3K/Akt/mTOR pathway

Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide‑3‑kinase/protein kinase B signaling pathway

Dendrobium: Sources of Active Ingredients to Treat Age-Related Pathologies

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