Gene expression and function involved in polyol biosynthesis of Trichosporonoides megachiliensis under hyper-osmotic stress

Kobayashi, Yasuhiko; Yoshida, Junjiro; Iwata, Hisashi; Koyama, Yoshiyuki; Ogihara, Jun; Kasumi, Takafumi

doi:10.1016/j.jbiosc.2012.12.004

Cited by 27 publications

(24 citation statements)

References 26 publications

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“…In S. cerevisiae, GPD1 (glyceraldehyde-3-phosphate dehydrogenase gene 1) is well known to modulate osmotic imbalance in the cell when exposed to a hyper-osmotic environment, but GPD2 apparently does not (28). Previously, we obtained similar results with the erythrose reductase (ER) isogenes, ER1, ER2, and ER3, derived from M. megachiliensis, and found that ER3 expression specifically was responsive to osmotic stress caused by glucose (20,29).…”

Section: Discussionsupporting

confidence: 54%

“…In contrast, M. megachiliensis produced glycerol and erythritol at essentially the same time upon exposure to osmotic stress. With longer exposure, however, erythritol became by far the more dominant compatible solute, as previously shown (10,20). Under conditions of oxidative stress, the profile of polyol production was different from the profile observed under osmotic stress conditions: glycerol accumulated rapidly, then erythritol accumulated gradually with time (Fig.…”

Section: Discussionmentioning

confidence: 52%

“…Intracellular polyols from M. megachiliensis were extracted and purified according to the method previously reported (20). The amount of polyols was amperometrically determined using an ICS-3000 (Dionex, Osaka, Japan) chromatographic system equipped with a Carbo Pac MA-1 column using isocratic elution with 0.5 M NaOH at a flow rate of 0.3 ml/min.…”

Section: Determination Of Intracellular Polyol Contentmentioning

confidence: 99%

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Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Iwata

Mizushima

Kobayashi

et al. 2015

Journal of Bioscience and Bioengineering

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 52%

Section: Determination Of Intracellular Polyol Contentmentioning

confidence: 99%

See 1 more Smart Citation

Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Iwata

Mizushima

Kobayashi

et al. 2015

Journal of Bioscience and Bioengineering

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“…; Kobayashi et al . ). Furthermore, after exhausting the glucose in the culture medium, M. megachiliensis begins to use glycerol and erythritol gradually as an energy source once excreted extracellulary (Kobayashi et al .…”

Section: Introductionmentioning

confidence: 97%

“…Furthermore, after exhausting the glucose in the culture medium, M. megachiliensis begins to use glycerol and erythritol gradually as an energy source once excreted extracellulary (Kobayashi et al . ). These findings suggest that M. megachiliensis may take glycerol up into the cell and convert it to erythritol via carbohydrate metabolism.…”

Section: Introductionmentioning

confidence: 97%

Erythritol production by M oniliella megachiliensis using nonrefined glycerol waste as carbon source

Kobayashi

Iwata

Mizushima

et al. 2015

Lett Appl Microbiol

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Significance and Impact of the Study: A shortage of C4 hydrocarbon depending much on naptha-oil has become urgent problem due to rapid reduction of naphtha plants together with global energy revolution. Erythritol, obtained by fermentation, is a rare C4 polyol that can be converted to C4 hydrocarbons. Erythritol is considerably expensive than hydrocarbons, a reduction in cost is critical issue. To meet this, we proposed to utilize low-cost glycerol waste from bio-diesel fuel as a carbon source. Moniliella megachiliensis successfully converted glycerol waste to erythritol. This proposal is promising to obtain C4 hydrocarbon substitute, and concomitantly to dispose a large amount of glycerol waste discharged. AbstractThe number of naphtha plants is being reduced due to a worldwide shift in energy sources. Consequently, a shortage of chemical materials heavily dependent on naphtha-oil, especially C4 compounds such as butene and butane-diol, is an urgent issue in chemical manufacturing. Erythritol is a rare C4 compound produced by fermentation processes using glucose as the carbon source. Since erythritol is considerably more expensive than hydrocarbons derived from naphtha-oil, a reduction in its cost is critical. We found that Moniliella megachiliensis, a highly osmotolerant yeast strain, can utilize nonrefined glycerol waste derived from palm oil or beef tallow and convert it to erythritol. Cell growth on glycerol was almost the same as on glucose, and the cells could grow in up to 300 mg ml À1 glycerol. When 200 mg ml À1 nonrefined glycerol was supplied, the yield of erythritol from the glycerol was approx. 60%, which is slightly higher than that obtained using glucose. The cost of glycerol waste is considerably lower than that of glucose. Thus, the conversion of glycerol waste into valuable erythritol, proposed here, is attractive and promising from the viewpoint of ensuring a supply of C4 hydrocarbons and utilizing a waste natural resource.

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Yeasts for Bioconversion of Crude Glycerol to High-Value Chemicals

Semkiv

Sibirny

2019

Non-Conventional Yeasts: From Basic Research to Application

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Gene expression and function involved in polyol biosynthesis of Trichosporonoides megachiliensis under hyper-osmotic stress

Cited by 27 publications

References 26 publications

Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class

Erythritol production by M oniliella megachiliensis using nonrefined glycerol waste as carbon source

Yeasts for Bioconversion of Crude Glycerol to High-Value Chemicals

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