2018
DOI: 10.1007/s00122-018-3166-7
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Abstract: Key messageCRISPR-Cas9-based genome editing and EMS mutagenesis revealed inter-cultivar differences and additivity in the contribution of TaGW2 homoeologues to grain size and weight in wheat.AbstractThe TaGW2 gene homoeologues have been reported to be negative regulators of grain size (GS) and thousand grain weight (TGW) in wheat. However, the contribution of each homoeologue to trait variation among different wheat cultivars is not well documented. We used the CRISPR-Cas9 system and TILLING to mutagenize each… Show more

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Cited by 131 publications
(83 citation statements)
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“…Indeed, variation in the GW2 gene in rice leads to grain weight differences of up to 50%, whereas a similar mutant in a single genome of the wheat ortholog (TaGW2-A) affects grain weight by only approximately 7% in wheat (Song et al 2007;Simmonds et al 2016). This hypothesis is supported by the fact that downregulating multiple homoeologs of TaGW2 has an additive effect, with simultaneous downregulation of all three homoeologs, by gene editing and mutants, increasing grain weight by 16.3% and 20.7%, respectively ( Figure 3B; Wang et al 2018b).…”
Section: Genetic Control Of Grain Weightmentioning
confidence: 84%
“…Indeed, variation in the GW2 gene in rice leads to grain weight differences of up to 50%, whereas a similar mutant in a single genome of the wheat ortholog (TaGW2-A) affects grain weight by only approximately 7% in wheat (Song et al 2007;Simmonds et al 2016). This hypothesis is supported by the fact that downregulating multiple homoeologs of TaGW2 has an additive effect, with simultaneous downregulation of all three homoeologs, by gene editing and mutants, increasing grain weight by 16.3% and 20.7%, respectively ( Figure 3B; Wang et al 2018b).…”
Section: Genetic Control Of Grain Weightmentioning
confidence: 84%
“…The expression level of TaGW7 was also evaluated in the plants carrying gene editing mutations in the B and D genomes (Figure b). These analyses revealed a substantial reduction of transcripts generated from the edited copies of the TaGW7 gene, which agrees with TaGW2 gene expression pattern from our previous study (Wang et al ., 2018b).…”
Section: Resultsmentioning
confidence: 99%
“…In wheat, CRISPR‐Cas9 gene editing was successfully applied to modify such traits of agronomic value as disease resistance (Wang et al ., ), gluten content (Sánchez‐León et al ., ), protein content (Zhang et al ., ), male sterility (Okada et al ., ) and grain size and weight (Wang et al ., ; Zhang et al ., , ). These studies also demonstrated that the editing of either individual copies of duplicated genes on homoeologous chromosomes or all three homoeologous copies of genes is possible (Wang et al ., 2014, 2018a).…”
Section: Introductionmentioning
confidence: 99%
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“…Presence of multiple copies of homoeologous genes may have consequence on phenotypic variation due to dosage effects and or functional redundancy (Borrill et al, 2019). Dosage effect, in which the phenotypic variation is amplified by the addition of each gene copies can act additively (e.g., genes controlling grain protein content (Avni et al, 2014) and grain size (Wang et al, 2018)) or non-additively (e.g., genes controlling amylopectin content in wheat (Kim et al, 2003)). Non-additive variation between homoeologous gene has been shown to be an important source of variation in wheat.…”
Section: Discussionmentioning
confidence: 99%