Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors

Shan, Yuebin; Luo, Ting; Peng, Chen; Sheng, Ruilong; Cao, Amin; Cao, Xueyan; Shen, Mingwu; Guo, Rui; Tomás, Helena; Shi, Xiangyang

doi:10.1016/j.biomaterials.2011.12.045

Cited by 224 publications

(191 citation statements)

References 57 publications

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“…The main challenge to date is delivery at the target site that is addressed by using viral and non-viral vector (Dufes et al 2005;Pakhare et al 2000). Viral systems have transfection capability, whereas on the other hand nonviral vectors have the capability to receive genetic material and transfer to the cell (Shan et al 2012). Dendrimers are well defined macromolecules consisting of globular structure.…”

Section: Dendrimers In Gene Deliverymentioning

confidence: 99%

A review on comparative study of PPI and PAMAM dendrimers

et al. 2016

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Dendrimers are hyperbranched, monodispersed macromolecules with multivalent functional end groups. Dendrimers have been explored as carrier for many drugs like anticancer, antiviral, antimalarial, antiprotozoal, anti tubercular drugs. Although a number of different types of dendrimers containing different core molecules, branching monomers and surface functional groups have been designed till date for drug delivery applications, yet the poly(propyleneimine) (PPI) and poly(amidoamine) (PAMAM) dendrimers have been the most explored dendrimers in this regard. In this review, we have summarized a comparative data on PPI and PAMAM dendrimers particularly relevant to their properties, synthesis, toxicity, biomedical applications and drug delivery attributes.

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Section: Dendrimers In Gene Deliverymentioning

confidence: 99%

A review on comparative study of PPI and PAMAM dendrimers

et al. 2016

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“…The past decades have seen an increasing interest in the use of various nanoparticulate systems for therapeutics (drug and gene delivery) [1][2][3][4][5][6], diagnostics [7][8][9][10], and the combination of both therapeutics and diagnostics (theranostics) [11][12][13]. In particular, the use of nanoparticles (NPs) as a drug carrier is able to overcome some of the current drawbacks of conventional cancer ligands, imaging agents, and anticancer drugs for simultaneous targeting, imaging, and therapeutic treatment of cancer cells [23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

RGD peptide-modified multifunctional dendrimer platform for drug encapsulation and targeted inhibition of cancer cells

Alves

Oliveira

et al. 2015

Colloids and Surfaces B: Biointerfaces

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100

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Development of multifunctional nanoscale drug-delivery systems for targeted cancer therapy still remains a great challenge. Here, we report the synthesis of cyclic arginine-glycine-aspartic acid (RGD) peptide-conjugated generation 5 (G5) poly(amidoamine) dendrimers for anticancer drug encapsulation and targeted therapy of cancer cells overexpressing αvβ3 integrins. In this study, amine-terminated G5 dendrimers were used as a platform to be sequentially modified with fluorescein isothiocyanate (FI) via a thiourea linkage and RGD peptide via a polyethylene glycol (PEG) spacer, followed by acetylation of the remaining dendrimer terminal amines. The developed multifunctional dendrimer platform (G5.NHAc-FI-PEG-RGD) was then used to encapsulate an anticancer drug doxorubicin (DOX). We show that approximately six DOX molecules are able to be encapsulated within each dendrimer platform. The formed complexes are water-soluble, stable, and able to release DOX in a sustained manner. One- and two-dimensional NMR techniques were applied to investigate the interaction between dendrimers and DOX, and the impact of the environmental pH on the release rate of DOX from the dendrimer/DOX complexes was also explored. Furthermore, cell biological studies demonstrate that the encapsulation of DOX within the G5.NHAc-FI-PEG-RGD dendrimers does not compromise the anticancer activity of DOX and that the therapeutic efficacy of the dendrimer/DOX complexes is solely related to the encapsulated DOX drug. Importantly, thanks to the role played by RGD-mediated targeting, the developed dendrimer/drug complexes are able to specifically target αvβ3 integrin-overexpressing cancer cells and display specific therapeutic efficacy to the target cells. The developed RGD peptide-targeted multifunctional dendrimers may thus be used as a versatile platform for targeted therapy of different types of αvβ3 integrin-overexpressing cancer cells.

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“…They suggested that the entrapment of gold nanoparticles within the dendrimer templates helped to preserve the 3D spherical shape of dendrimers, enabling high compaction of DNA. An important observation was that dendrimer-entrapped gold nanoparticles had lower cytotoxicity compared with the dendrimers without nanoparticles [64].…”

Section: Handbook Of Nanoparticlesmentioning

confidence: 99%

Bio-Functionalized Metallic Nanoparticles with Applications in Medicine

Pruneanu¹,

Coroș²,

Pogăcean³

2015

Handbook of Nanoparticles

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Due to their special physicochemical, optical, and biological properties, noble metal nanoparticles have huge potential for application in many different biological and medical areas, such as highly sensitive diagnostic assay, thermal ablation, radiotherapy, or carriers for drugs and gene delivery. This chapter selectively reviews the bio-functionalization of metallic nanoparticles and their recent applications in medicine. The chapter is divided into four sections: Introduction, Bioconjugation of Metallic Nanoparticles, Cancer Therapy, and Gene Delivery. After a short introduction, we present few general strategies for bioconjugation of metallic nanoparticles: physisorption, physisorption using mediator molecules, covalent binding of biomolecules to cross-linkers, covalent binding of biomolecules to nanoparticles, and linking of biotinylated biomolecules to streptavidin-functionalized nanoparticles. The third section presents the recent advances in cancer therapy based on two strategies: passive targeting and antibody targeting, using functionalized gold nanoparticles. The fourth section describes the gene delivery process, by which foreign DNA is introduced into the host cells. The process typically involves the formation of transient pores or "holes" into the cell membrane, which allows the uptake of foreign material. The main aspects that are discussed about the gene delivery process are the stealth character and the targeted recognition of tissues.

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Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors

Cited by 224 publications

References 57 publications

A review on comparative study of PPI and PAMAM dendrimers

A review on comparative study of PPI and PAMAM dendrimers

RGD peptide-modified multifunctional dendrimer platform for drug encapsulation and targeted inhibition of cancer cells

Bio-Functionalized Metallic Nanoparticles with Applications in Medicine

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