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Cited by 1 publication
(1 citation statement)
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“…After centrifugation, the pellet was washed with cold PBS, suspended in 500 µl PBS, and incubated with 5 µl RNase (20 µg ml −1 final concentration) for 30 min. The cells were kept on ice for 10 min and incubated with propidium iodide (50 µg ml −1 final concentration) for 1 h in the dark [73], [74]. The cell cycle distribution of the cells of each sample was then determined using a FACSCalibur instrument (BD Biosciences, San Jose, CA) equipped with CellQuest 3.3 software.…”
Section: Methodsmentioning
confidence: 99%
“…After centrifugation, the pellet was washed with cold PBS, suspended in 500 µl PBS, and incubated with 5 µl RNase (20 µg ml −1 final concentration) for 30 min. The cells were kept on ice for 10 min and incubated with propidium iodide (50 µg ml −1 final concentration) for 1 h in the dark [73], [74]. The cell cycle distribution of the cells of each sample was then determined using a FACSCalibur instrument (BD Biosciences, San Jose, CA) equipped with CellQuest 3.3 software.…”
Section: Methodsmentioning
confidence: 99%