GC–MS and LC–MS approaches for determination of tocopherols and tocotrienols in biological and food matrices

Bartosińska, Ewa; Buszewska-Forajta, Magdalena; Siluk, Danuta

doi:10.1016/j.jpba.2016.02.051

Cited by 52 publications

(36 citation statements)

References 88 publications

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“…As it is recommended to use at least two methods for measuring total antioxidant capacity (Apak et al, 2013;Bartosi nska, Buszewska-Forajta, & Siluk, 2016), the ferric reducing antioxidant power (FRAP) assay developed by Benzie and Strain (1996) was also performed on the sample extracts.…”

Section: Ferric Reducing Antioxidant Powermentioning

confidence: 99%

Antioxidative properties and macrochemical composition of five commercial mungbean varieties in Australia

et al. 2020

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Mungbeans are growing in popularity among Australian consumers, driven by their beneficial nutritional and phytochemical composition. However, data on the antioxidative, mineral, and phytochemical content of Australian mungbeans at the point of consumer purchase remains scarce. Here, five commercial mungbean samples were analysed for total antioxidant capacity, total phenolics, and total monomeric anthocyanins. Attenuated total reflectance midinfrared spectroscopy was utilised as a rapid and reliable method of obtaining information about the macrochemical composition of the mungbean hulls. Total antioxidant capacity ranged from 170 to 570 mg Trolox equivalents per 100 g, total phenolic content from 130 to 240 mg gallic acid equivalents per 100 g, and anthocyanin content from 10 to 40 mg cyanidin‐3‐glucoside equivalents per 100 g. There was a significant difference between varieties in all measures of antioxidant, phenolic and anthocyanin contents. Using principal component analysis, the midinfrared spectra for the five mungbean varieties could be isolated, highlighting the differences in their phytochemical composition. In general, whole Australian mungbeans appear to have the highest antioxidant, phenolic, and anthocyanin contents. Midinfrared spectroscopy appears to be a valuable method of obtaining and comparing the macrochemical composition of mungbeans. This technology is likely to be of increasing use in the future.

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Section: Ferric Reducing Antioxidant Powermentioning

confidence: 99%

Antioxidative properties and macrochemical composition of five commercial mungbean varieties in Australia

et al. 2020

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“…Since tocochromanol derivatives are weakly ionizing analytes, the main drawback of LC/MS methods for their determination is related to poor reproducibility of the ionization process, which consequently affects each step of analytical method validation. For this reason LC/MS is rarely used for the quantification of T and T3 in biological and food matrices …”

Section: Introductionmentioning

confidence: 99%

“…For this reason LC/MS is rarely used for the quantification of T and T3 in biological and food matrices. 15 So far, literature reports concerning simultaneous analysis of T and T3 with the use of LC/MS [16][17][18][19][20][21][22] are scarce. Likewise, works describing the ion source optimization in the case of simultaneous determination of multiple tocochromanols are limited.…”

Section: Introductionmentioning

confidence: 99%

Ionization of tocopherols and tocotrienols in atmospheric pressure chemical ionization

Bartosińska

Moor

Siluk

et al. 2018

Rapid Comm Mass Spectrometry

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“…Qualitative and quantitative determination of T and T3 in various matrices is usually performed with the application of fluorescence (FLD) (Chajès, Lhuillery, Sattler, Kostner, & Bougnoux, ; Handelman, Epstein, Machlin, van Kuijk, & Dratz, ; Rupérez, Barbas, Castro, Martínez, & Herrera, ; Shim et al, ; Takahashi et al, ; Weiser, Riss, & Kormann, ; Yeum et al, ) and diode‐array detection (Gleize, Steib, André, & Reboul, ; Yang et al, ; Yuan, Ju, Jin, Ren, & Liu, ). Mass spectrometry (MS) is rarely implemented in determination of T and T3 (Ansolin, de Souza, de Almeida Meirelles, & Batista, ; Bartosińska, Buszewska‐Forajta, & Siluk, ; Nagy et al, ; Viñas et al, ) as the ionization process of these compounds is problematic. To our best knowledge, few authors have described the use of LC–MS methods for the simultaneous quantification of more than one tocochromanol, for instance the normal‐phase liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry (NP–LC–APCI–MS) method presented by Nagy et al (), the RP–LC–APCI–MS method described by Viñas et al (), the reversed‐phase liquid chromatography coupled with electrospray ionization mass spectrometry (RP–LC–ESI–MS) method by Bustamante‐Rangel, Delgado‐Zamarreño, Sánchez‐Pérez, & Carabias‐Martínez () or the RP–UPLC–ESI–MS n method presented by Górnaś, Pugajeva, & Segliņa ().…”

Section: Introductionmentioning

confidence: 99%

“…The authors reported increased sensitivity of the APPI method in reference to ESI‐ or APCI‐based analytical methods used for tocochromanol quantification. However, a significant number of other studies refer to quantification of a few tocols or one of the tocopherols along with other lipophilic constituents (Bartosińska et al, ). Moreover, some authors indicated problematic efficiency and repeatability of tocochromanol ionization in both ESI (Bustamante‐Rangel et al, ) and APCI (Bartosińska, Borsuk‐De Moor, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Bartosińska

Jacyna

Moor

et al. 2018

Biomedical Chromatography

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Tocopherols and tocotrienols have been extensively studied owing to their anticancer potential, especially against breast cancer. Therefore, the aim of this study was to quantitatively determine tocochromanols in human breast adipose tissue with the use of HPLC-FLD. The sample preparation procedure included homogenization and solvent extraction with isopropanol-ethanol-0.1% formic acid mixture prior to solid-phase extraction. After implementation of central composite design, satisfactory separation of all eight target compounds was achieved within 10.5 min. Chromatographic runs were carried out with the use of a naphthylethyl chromatographic column with methanol-water mixture (89:11, v/v) as the mobile phase. Fluorescence detection of tocochromanols was performed with excitation and emission wavelengths 298 and 330 nm, respectively. The method was validated in terms of linearity, carryover, recovery, precision, accuracy and stability. Extraction yield was also determined for accurate evaluation of vitamin E content in human breast adipose tissue samples. Finally, concentrations of particular tocochromanols compounds were assessed in human breast adipose tissue samples obtained from 99 patients, including women with breast cancer, healthy volunteers and deceased women who had died as a result of accidents. The raw data was transformed according to the newly developed equation for accurate estimation of the concentrations of tocochromanols in breast adipose tissue samples. Results obtained in the study indicated that the proposed analytical assay could be useful in breast cancer research.

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GC–MS and LC–MS approaches for determination of tocopherols and tocotrienols in biological and food matrices

Cited by 52 publications

References 88 publications

Antioxidative properties and macrochemical composition of five commercial mungbean varieties in Australia

Antioxidative properties and macrochemical composition of five commercial mungbean varieties in Australia

Ionization of tocopherols and tocotrienols in atmospheric pressure chemical ionization

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

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