GbFT, a FLOWERING LOCUS T homolog from Ginkgo biloba, promotes flowering in transgenic Arabidopsis

Wang, Lanlan; Yan, Jiaping; Zhou, Xian; Cheng, Shuiyuan; Chen, Zexiong; Song, Qiling; Liu, Xiaomeng; Ye, Jiabao; Zhang, Weiwei; Wu, Guangxu; Xu, Feng

doi:10.1016/j.scienta.2018.12.020

Cited by 12 publications

(9 citation statements)

References 42 publications

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“…Phenylpropanoids are precursors of flavonoids that play vital roles in flower development and the defense against oxidative stress and abiotic stimuli 39 . “Cutin, suberine and wax biosynthesis” may be related to flower organ development in transgenic Arabidopsis plants 40 . Unigenes with higher expression in seed were mainly enriched in the “biosynthesis of unsaturated fatty acids” pathway.…”

Section: Discussionmentioning

confidence: 99%

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Ruan

Wang

et al. 2022

Sci Rep

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Mikania micrantha is a notorious invasive weed that has caused huge economic loss and negative ecological consequences in invaded areas. This species can adapt well to invasive environments with various stress factors. The identification of gene families and functional pathways related to environmental adaptability is lack in M. micrantha at the multi-organ full-length transcriptome level. In this study, we sequenced the transcriptomes of five M. micrantha organs using PacBio single-molecule real-time sequencing and Illumina RNA sequencing technologies. Based on the transcriptome data, full-length transcripts were captured and gene expression patterns among the five organs were analyzed. KEGG enrichment analysis of genes with higher expression indicated their special roles in environmental stress response and adversity adaptation in the various five organs. The gene families and pathways related to biotic and abiotic factors, including terpene synthases, glutathione S-transferases, antioxidant defense system, and terpenoid biosynthesis pathway, were characterized. The expression levels of most differentially expressed genes in the antioxidant defense system and terpenoid biosynthesis pathway were higher in root, stem, and leaf than in the other two organs, suggesting that root, stem, and leaf have strong ability to respond to adverse stresses and form the important organs of terpenoid synthesis and accumulation. Additionally, a large number of transcription factors and alternative splicing events were predicted. This study provides a comprehensive transcriptome resource for M. micrantha, and our findings facilitate further research on the adaptive evolution and functional genomics of this species.

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Section: Discussionmentioning

confidence: 99%

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Ruan

Wang

et al. 2022

Sci Rep

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“…About 15-day-old seedlings were transferred into soil and grown under long-day condition. T 1 and T 2 transgenic plants were further confirmed by PCR and β-glucuronidase activity, referring to the method of Wang et al (2019) . T 3 seeds from T 2 transgenic plants were harvested following the screening by the MS plates with 100 mg/L kanamycin.…”

Section: Methodsmentioning

confidence: 99%

“…Further, DELLAs interacted with SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) to interfere SPL transcriptional activity and affect flowering time (Yu et al, 2012). At present, some flowering-related genes have been identified in G. biloba, such as GbCO (Yan et al, 2017), GbFT (Wang et al, 2019), GbMADS2 (Wang et al, 2015), GbSEP (Cheng et al, 2016), and GbMADS9 (Yang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of the EMBRYONIC FLOWER 2 Gene Involved in Flowering in Ginkgo biloba

et al. 2021

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Ginkgo biloba has edible, medicinal, and ornamental value. However, the long juvenile phase prevents the development of the G. biloba industry, and there are few reports on the identification and functional analysis of genes regulating the flowering time of G. biloba. EMBRYONIC FLOWER 2 (EMF), an important protein in flower development, functions to promote vegetative growth and repress flowering. In this study, a novel EMF gene (GbEMF2) was cloned and characterized from G. biloba. GbEMF2 contains a 2,193 bp open reading frame (ORF) encoding 730 amino acids. GbEMF2 harbors conserved VEFS-Box domain by the plant EMF protein. The phylogenic analysis showed that GbEMF2 originated from a polycomb-group (Pc-G) protein ancestor and was a member of the EMF2 protein. The quantitative real-time PCR (qRT-PCR) analysis revealed that GbEMF2 was expressed in all detected organs, and it showed a significantly higher level in ovulating strobilus and microstrobilus than in other organs. Compared with emf2 mutant plants, overexpression of GbEMF2 driven by the CaMV 35S promoter in emf2 mutant Arabidopsis plants delayed flowering but earlier than wild-type (WT) plants. This result indicated that GbEMF2 repressed flowering in G. biloba. Moreover, the RNA-seq analysis of GbEMF2 transgenic Arabidopsis plants (GbEMF2-OE/emf2), WT plants, and emf2 mutants screened out 227 differentially expressed genes (DEGs). Among these DEGs, FLC, MAF5, and MAF5-1 genes were related to flower organ development and regulated by GbEMF2. In addition, some genes participating in sugar metabolism, such as Alpha-amylase 1 (AMY1), BAM1, and Sucrose synthase 3 (SUS3) genes, were also controlled by GbEMF2. Overall, our results suggested that GbEMF2 negatively regulates flowering development in G. biloba. This finding provided a foundation and target gene for shortening the Ginkgo juvenile period by genetic engineering technology.

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“…Few studies provided information about genetic mechanism of flowering in G. biloba . Although our previous work cloned and function reported some genes involved in flower development including three MADS-box transcription factor genes [ 26 – 28 ] and two circadian-regulated genes ( GbCO and GbFT ) in photoperiodic pathway [ 4 , 29 ]. However, little literature reported genetic regulation of sexual differentiation and sexual determination of G. biloba , especially for miRNA identification of strobili sexual differentiation and its regulatory mechanism.…”

Section: Introductionmentioning

confidence: 99%

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, RNA, and degradome sequencing

Liu

Cheng

et al. 2020

BMC Plant Biol

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Background: Ginkgo biloba, a typical dioecious plant, is a traditional medicinal plant widely planted. However, it has a long juvenile period, which severely affected the breeding and cultivation of superior ginkgo varieties. Results: In order to clarify the complex mechanism of sexual differentiation in G. biloba strobili. Here, a total of 3293 miRNAs were identified in buds and strobili of G. biloba, including 1085 known miRNAs and 2208 novel miRNAs using the three sequencing approaches of transcriptome, small RNA, and degradome. Comparative transcriptome analysis screened 4346 and 7087 differentially expressed genes (DEGs) in male buds (MB) _vs_ female buds (FB) and microstrobilus (MS) _vs_ ovulate strobilus (OS), respectively. A total of 6032 target genes were predicted for differentially expressed miRNA. The combined analysis of both small RNA and transcriptome datasets identified 51 miRNA-mRNA interaction pairs that may be involved in the process of G. biloba strobili sexual differentiation, of which 15 pairs were verified in the analysis of degradome sequencing. Conclusions: The comprehensive analysis of the small RNA, RNA and degradome sequencing data in this study provided candidate genes and clarified the regulatory mechanism of sexual differentiation of G. biloba strobili from multiple perspectives.

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GbFT, a FLOWERING LOCUS T homolog from Ginkgo biloba, promotes flowering in transgenic Arabidopsis

Cited by 12 publications

References 42 publications

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Functional Characterization of the EMBRYONIC FLOWER 2 Gene Involved in Flowering in Ginkgo biloba

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, RNA, and degradome sequencing

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