Gastrointestinal Motility and Gut Hormone Secretion in response to Shenhuang Plaster in a Postoperative Ileus Rat Model

Abstract

Postoperative ileus (POI), a gastrointestinal function disorder, is a complication that arises from surgery. Shenhuang plaster (SHP) application to the Shenque acupoint (CV8) to promote the recovery of gastrointestinal function has achieved definite curative effects in clinical settings; however, the underlying pharmacological mechanism remains unknown. In this study, we evaluated the effects of SHP using a Sprague Dawley rat POI model. Then, gastrointestinal transit in different rat groups was evaluated by the movement of fluorescein-labelled dextran. Ghrelin, obestatin, motilin (MTL), and vasoactive intestinal peptide (VIP) plasma concentrations were measured via a radioimmunoassay. The expression of the ghrelin and obestatin receptors (GHS-R1α and GPR39) in the intestinal muscularis of rats in different groups was comparatively identified via western blotting. The results indicated that SHP application improved gastrointestinal motility in POI model rats. SHP application significantly increased ghrelin concentration and the expression of its receptor and inhibited obestatin concentration and the expression of its receptor in blood. Further, ghrelin concentration and the capability of gastrointestinal transit were positively correlated. Simultaneously, SHP application also promoted the secretion of other gastrointestinal motility hormones, such as MTL and VIP. Hence, these results provide evidence that SHP can promote the recovery of gastrointestinal transmission in POI rat models through regulation of ghrelin and other intestinal hormones.

Effect of dexamethasone pretreatment using deep learning on the surgical effect of patients with gastrointestinal tumors

“…Early recovery of gastrointestinal function after gastrointestinal surgery is clinically important because it reduces the incidence of postoperative ileus (POI). POI is associated with postoperative pain, increased nausea and vomiting, and increased treatment costs [ 27 ]. This work confirmed that FET in Exp group patients was much shortened compared with the FET in the Ctrl group patients ( P <0.05).…”

Exploring the chemical components of Kuanchang-Shu granule and its protective effects of postoperative ileus in rats by regulating AKT/HSP90AA1/eNOS pathway

“…It is noteworthy that a large number of effective schemes are provided for clinical treatment along with vigorous progression research of combination of traditional Chinese medicine and western medicine [ 5 ]. Several formulas, single herb or active ingredient of traditional Chinese medicines, such as Shenhuang plaster [ 6 ], Zingiberis Siccatum Rhizoma [ 7 ], Cistanche deserticola [ 8 ] and hesperidin [ 9 ], had played unique advantages in the treatment of gastrointestinal diseases, whose mechanisms were related to inhibiting inflammatory responses by regulating ghrelin and other intestinal hormones, activating the transient receptor potential A1 (TRPA1) channels in entero-chromaffin cells, stimulating interstitial Cajal cells and stimulating Ca 2+ -dependent myosin phosphorylation. Among them, Da-Cheng-Qi Decoction (DCQD) is a representative laxative prescription with remarkable effect recorded in “Shang Han Lun”, which can effectively improve the inflammatory symptoms of intestinal peristalsis and obstruction, alleviate gastrointestinal paralysis, and improve gastrointestinal motor function with a rapid and highly potent effect [ 10 , 11 ].…”