Gastrointestinal digestion of hazelnut allergens on molecular level: Elucidation of degradation kinetics and resistant immunoactive peptides using mass spectrometry

Korte, Robin; Bräcker, Julia; Brockmeyer, Jens

doi:10.1002/mnfr.201700130

Cited by 22 publications

(23 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, Campana variety showed only an additional band with mass of approximately 33-35 kDa in the processed sample compared to the raw extract. On the contrary, in Romana and Georgia roasted extracts, the band at 50 kDa belonging to Cor a 11 allergen became weak compared to the relative raw sample [39]. Moreover, in Georgia roasted sample, a new band of approximately 73 kDa was highlighted, although with a weak signal.…”

Section: Varietal Diversity Andmentioning

confidence: 83%

Effects of the Varietal Diversity and the Thermal Treatment on the Protein Profile of Peanuts and Hazelnuts

Angelis

Bavaro

Monaci

et al. 2018

Journal of Food Quality

View full text Add to dashboard Cite

Several bu er compositions were compared for their e ciency in protein extraction from both raw and roasted peanut and hazelnut samples, the nal goal being to understand the modi cation of protein solubility upon roasting and maximize the extraction yield. Denaturant conditions provided by urea-TBS bu er resulted in satisfactory extraction yields for both peanut and hazelnut samples, before and after the thermal treatment. In addition, di erent varieties of peanuts and hazelnuts were characterized to highlight the extent of variability in the protein pro le accounted by the varietal factor and eventual di erential resistance among cultivars to protein modi cation induced by the thermal processing. e protein pro le was characterized by gel electrophoresis, and speci c bands were analyzed by micro-HPLC-MS/MS coupled to software-based protein identi cation. No signi cant di erence was observed for the investigated hazelnut cultivars, namely, Campana, Romana, and Georgia, whereas interesting features were presented for the peanut varieties Virginia, Zambia, and China. In particular, Zambia variety lacked two bands of approximately 36 and 24 kDa that were visible in Virginia and China varieties, which could suggest a lower allergenic potential of this particular variety which deserves to be further investigated before drawing nal conclusions.

show abstract

Section: Varietal Diversity Andmentioning

confidence: 83%

Effects of the Varietal Diversity and the Thermal Treatment on the Protein Profile of Peanuts and Hazelnuts

Angelis

Bavaro

Monaci

et al. 2018

Journal of Food Quality

View full text Add to dashboard Cite

show abstract

“…However, it deserves to be noted that here the peptide identification is complicated by the availability of full-length protein sequences from on-line databases and by the heterogeneity of the peptide mixture generated with not predictable specificity 31 . Given the high complexity of the enzyme mixtures used for GDD an ‘unspecific cleavage’ was set for peptide identification together with a wide range in the expected peptide lengths and molecular weight 61,62 . Such comprehensive set up for peptide searching would have raised exponentially the amount of data processing; therefore, in order to constrain the final software output to only the information fitting our purposes, we selected a customized restricted protein database, for specific searches.…”

Section: Resultsmentioning

confidence: 99%

Scouting for Naturally Low-Toxicity Wheat Genotypes by a Multidisciplinary Approach

Pilolli

Gadaleta

Mamone

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Over the last years, great efforts have been devoted to develop effective gluten detoxification strategies with a consequent detrimental alteration of the technological properties as well. Obtaining low-gluten products without affecting the rheological properties of wheat could still be considered a new challenge to face. In this investigation, we presented a comprehensive characterization of durum wheat genotypes aimed at identifying low gluten ones, which combine the potential lower toxicity/immunogenicity with conserved yield and rheological properties to encompass the perspective usability for bread or pasta making. A preliminary profiling of gluten proteins was accomplished by immunoassay-based quantification and liquid chromatography coupled to UV detection, focusing on the gliadin fraction as main responsible for immunoreactivity in celiac disease patients. In addition, data on grain protein content, grain yield per spike, dry gluten and gluten index were collected in order to provide complementary information about productivity-related traits and quali-quantitative characteristics related to wheat nutritional value and its technological properties. The whole pool of data was statistically evaluated driving to the selection of a preferred list of candidate low-toxicity genotypes that were subjected to in-vitro simulated gastroduodenal digestion and untargeted HR-MS/MS peptide identification. Finally, an in-silico risk assessment of potential toxicity for celiac disease patients was performed according to the most recent guidance provided by EFSA.

show abstract

“…In the present study, F. ruminococcaceae and F. prausnitzii, belonging to the genus Faecalibacterium, were significant lower in the PTB group than in healthy controls. Previous studies have described that F. ruminococcaceae and F. prausnitzii may exert beneficial effects on human health though their metabolites, short-chain fatty acids (SCFA) [31, 32]. SCFAs are able to affect lipid, glucose, and cholesterol metabolism in many tissues, where a large proportion are used as a source of energy [33].…”

Section: Discussionmentioning

confidence: 99%

Characterization of gut microbiota in children with pulmonary tuberculosis

Zhu

Liao

et al. 2019

BMC Pediatr

View full text Add to dashboard Cite

BackgroundGut microbiota plays a critical role in many important physiological processes and is linked with various pulmonary infectious diseases. The relationship between pulmonary tuberculosis (PTB) and gut microbiota has been poorly studied. The present study aimed to characterize gut microbiota in pediatric patients with PTB.MethodsA case-controlled study was executed for the characterization of gut microbiota in pediatric PTB patients. Fecal samples were collected from the PTB patients and healthy controls upon admission. In addition, a one-month follow-up assessment was performed to investigate alterations in the gut microbiota post anti-tuberculosis treatment. 16SrDNA sequencing analysis of fecal DNA was completed on the Illumina MiSeq platform.ResultsCompared with healthy controls, the gut microbiota of pediatric patients with PTB was characterized by decreased microbial diversity. PTB patients further presented an up-regulation of the pro-inflammatory bacteria Prevotella, the opportunistic pathogen Enterococcus, as well as a reduction of beneficial bacteria including Ruminococcaceae, Bifidobacteriaceae and prausnitzii. One-month after anti-tuberculosis therapy, the richness of gut microbiota in PTB patients was distinctly depleted.ConclusionsThe gut microbiota of pediatric patients with PTB was significantly distinct from healthy controls. Additionally, the richness of gut microbiota in PTB patients decreased after one-month anti-tuberculosis treatment. It is hypothesized that the homeostasis of gut microbiota in PTB patients may affect the pathogenies of PTB by de-regulation of the hosts’ immune status through the gut-lung axis.

show abstract

Gastrointestinal digestion of hazelnut allergens on molecular level: Elucidation of degradation kinetics and resistant immunoactive peptides using mass spectrometry

Cited by 22 publications

References 65 publications

Effects of the Varietal Diversity and the Thermal Treatment on the Protein Profile of Peanuts and Hazelnuts

Effects of the Varietal Diversity and the Thermal Treatment on the Protein Profile of Peanuts and Hazelnuts

Scouting for Naturally Low-Toxicity Wheat Genotypes by a Multidisciplinary Approach

Characterization of gut microbiota in children with pulmonary tuberculosis

Contact Info

Product

Resources

About