Gas-liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid

James, A. T.; Martin, A. J. P.

doi:10.1042/bj0500679

Cited by 1,733 publications

(401 citation statements)

References 5 publications

(8 reference statements)

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“…One part was evaporated to small volume and continuously extracted with ether for some hours; the ether extract was chromatographed on paper in butanol + acetic acid and in butanol + ammonia (sprayed with bromcresol green and bromthymol blue respectively). The other part was steam distilled after adding an equal volume of 2~-sulphuric acid, and the volatile acids in the distillate identified by gas-phase chromatography (James & Martin, 1952 …”

Section: P H a Sneathmentioning

confidence: 99%

Cultural and Biochemical Characteristics of the Genus Chromobacterium

Sneath

1956

Journal of General Microbiology

160

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SUMMARY: The cultural and biochemical characteristics of 38 strains of Chromobacterium are described. They are non-sporing aerobic Gram-negative rods which produce a violet pigment and possess both polar monotrichous and peritrichous flagella. They produce little acidity fkom carbohydrates and utilize citrate.T w o main groups of strains were distinguishable, a mesophilic and a psychrophilic group. The former usually ferment glucose anaerobically, are markedly proteolytic, facultatively anaerobic, and give a positive egg-yolk reaction, but do not hydrolyse aesculin. The psychrophilic organisms produce acid fkom glucose but only aerobically, are poorly proteolytic, strictly aerobic, and hydrolyse aesculin but do not produce an egg-yolk reaction.

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Section: P H a Sneathmentioning

confidence: 99%

Cultural and Biochemical Characteristics of the Genus Chromobacterium

Sneath

1956

Journal of General Microbiology

160

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“…Peaks were identified by comparison of their retention times with those of authentic standards, and in the case of the fatty acids by comparing retention times on a James plot (James & Martin, 1952). Materials.…”

Section: B a R C H E R Methodsmentioning

confidence: 99%

Modification of the Membrane Composition of Mycoplasma mycoides subsp. capri by the Growth Medium

Archer¹

1975

Journal of General Microbiology

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S U M M A R YMycoplasma mycoides subsp. Capri was grown in different media. The amounts of the lipids in these media were varied, resulting in altered lipid compositions of the cells. Lowering the amounts of cholesterol in the media resulted in less cholesterol incorporation into the cell lipid, with a concomitant decrease in the amount of phospholipid. The changes in the phospholipid/cholesterol ratio in the mycoplasmas were very small compared with the large changes in the amount of cholesterol which occurred when the cholesterol content in the medium was altered. Changes in the amounts of glycolipid and glyceride in the cell lipid also resulted from alterations of the cholesterol concentration in the media. Under these conditions cells with reduced cholesterol contents were more sensitive to lysis by digitonin. No changes were observed in the polyacrylamide gel electrophoretic pattern of cellular or membrane proteins when the cholesterol was replaced by other sterols.

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“…The time that any solute spends in the mobile phase, that is the unretained peak time (t M ), summed with the socalled adjusted retention time ( ), which corresponds to the time the solute spends distributed into the stationary phase, is equivalent to the retention time (t R ) [see equation (1)]:…”

Section: Principles Of Gas Chromatographic Retention Parametersmentioning

confidence: 99%

Linear retention indices in gas chromatographic analysis: a review

Zellner

Bicchi

Dugo

et al. 2008

Flavour & Fragrance J

197

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ABSTRACT:The main purpose of any chromatographic analysis is to resolve mixtures of compounds into less complex mixtures or ultimately into pure components. In addition to this function, the chromatographic system can provide retention data which serve as complementary information for the positive identification of resolved components. The need to express gas chromatographic retention data in a standardized system has long been recognized and retention index values presented to be a valuable parameter. Those values are mainly calculated by applying the equations proposed by Kováts, for isothermal analysis, and van den Dool and Kratz, for programmed gas chromatographic runs. In general, these indices denote the retention behaviour of the compounds of interest according to a uniform scale determined by a series of closely related standard substances. The use of retention indices in the flavour and fragrance field is well-documented, and they are widely applied for the comparison of results between laboratories, as well as to characterize stationary phases.

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Gas-liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid

Cited by 1,733 publications

References 5 publications

Cultural and Biochemical Characteristics of the Genus Chromobacterium

Cultural and Biochemical Characteristics of the Genus Chromobacterium

Modification of the Membrane Composition of Mycoplasma mycoides subsp. capri by the Growth Medium

Linear retention indices in gas chromatographic analysis: a review

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