Gas-liquid chromatoographic properties of catecholamines, phenylethylamines and indolalkylamines as their propionyl derivatives

In Matrix Derivatization Combined with LC-MS/MS Results in Ultrasensitive Quantification of Plasma Free Metanephrines and Catecholamines

“…This is illustrated for dopamine in Figure S-6, which shows the product ion spectrum at collision energies of 15 and 30 eV. Propionylation was shown to be effective in aqueous medium for gas chromatographic analysis of biogenic amines and showed advantages over the more reactive fluorinated reagents. − . However, in this study, we did not perform in situ derivatization in plasma but only in neat solutions and with pyridine as a catalyst.…”

“…This study proves that catecholamines and metanephrines can be propionylated directly in plasma, which greatly improves their detection by mass spectrometry. This increase in sensitivity is probably related to the increased lipophilicity of the derivatives and subsequent increase in ionization efficiency. , Furthermore, the method is highly automated, which reduces possible human errors. It can be improved even more by converting the liquid chromatography method to ultraperformance liquid chromatography.…”

In Matrix Derivatization Combined with LC-MS/MS Results in Ultrasensitive Quantification of Plasma Free Metanephrines and Catecholamines

“…This is illustrated for dopamine in Figure S-6, which shows the product ion spectrum at collision energies of 15 and 30 eV. Propionylation was shown to be effective in aqueous medium for gas chromatographic analysis of biogenic amines and showed advantages over the more reactive fluorinated reagents. − . However, in this study, we did not perform in situ derivatization in plasma but only in neat solutions and with pyridine as a catalyst.…”

“…This study proves that catecholamines and metanephrines can be propionylated directly in plasma, which greatly improves their detection by mass spectrometry. This increase in sensitivity is probably related to the increased lipophilicity of the derivatives and subsequent increase in ionization efficiency. , Furthermore, the method is highly automated, which reduces possible human errors. It can be improved even more by converting the liquid chromatography method to ultraperformance liquid chromatography.…”

Gas Chromatographic Analysis of Histamine in Mahi-mahi ( Coryphaena hippurus )

“…It is known, however, that the GC-ECD is more sensitive than the GC-FID, particularly toward electronegative functional groups such as halogens (48). Hiemke et al (49) reported that the derivatized histamine is unstable in the presence of water and that up to 50% of the derivatives can be lost during preparation. Our observations showed that the histamine derivative was stable for short periods (2-3 days at refrigeration temperature), after which time smaller and broader peaks were seen in the chromatograms.…”

Distribution and Properties of Thiol S‐Methyltransferase in Rat Brain

“…A toluene-based scintillant (3 ml) was added, and the radioactivity measured by liquid scintillation counting. When cysteine or homocysteine was used as substrate, the amino groups were propionylated (Hiemke et al, 1978) prior to extraction. Methylated mercaptoethylamine was removed from the aqueous phase by liquid ion-exchange extraction.…”

In Matrix Derivatization Combined with LC-MS/MS Results in Ultrasensitive Quantification of Plasma Free Metanephrines and Catecholamines

“…This is illustrated for dopamine in Figure S-6, which shows the product ion spectrum at collision energies of 15 and 30 eV. Propionylation was shown to be effective in aqueous medium for gas chromatographic analysis of biogenic amines and showed advantages over the more reactive fluorinated reagents. − . However, in this study, we did not perform in situ derivatization in plasma but only in neat solutions and with pyridine as a catalyst.…”

“…This study proves that catecholamines and metanephrines can be propionylated directly in plasma, which greatly improves their detection by mass spectrometry. This increase in sensitivity is probably related to the increased lipophilicity of the derivatives and subsequent increase in ionization efficiency. , Furthermore, the method is highly automated, which reduces possible human errors. It can be improved even more by converting the liquid chromatography method to ultraperformance liquid chromatography.…”

Gas Chromatographic Analysis of Histamine in Mahi-mahi ( Coryphaena hippurus )

“…It is known, however, that the GC-ECD is more sensitive than the GC-FID, particularly toward electronegative functional groups such as halogens (48). Hiemke et al (49) reported that the derivatized histamine is unstable in the presence of water and that up to 50% of the derivatives can be lost during preparation. Our observations showed that the histamine derivative was stable for short periods (2-3 days at refrigeration temperature), after which time smaller and broader peaks were seen in the chromatograms.…”

Distribution and Properties of Thiol S‐Methyltransferase in Rat Brain

“…A toluene-based scintillant (3 ml) was added, and the radioactivity measured by liquid scintillation counting. When cysteine or homocysteine was used as substrate, the amino groups were propionylated (Hiemke et al, 1978) prior to extraction. Methylated mercaptoethylamine was removed from the aqueous phase by liquid ion-exchange extraction.…”

In Matrix Derivatization Combined with LC-MS/MS Results in Ultrasensitive Quantification of Plasma Free Metanephrines and Catecholamines

“…This is illustrated for dopamine in Figure S-6, which shows the product ion spectrum at collision energies of 15 and 30 eV. Propionylation was shown to be effective in aqueous medium for gas chromatographic analysis of biogenic amines and showed advantages over the more reactive fluorinated reagents. − . However, in this study, we did not perform in situ derivatization in plasma but only in neat solutions and with pyridine as a catalyst.…”

“…This study proves that catecholamines and metanephrines can be propionylated directly in plasma, which greatly improves their detection by mass spectrometry. This increase in sensitivity is probably related to the increased lipophilicity of the derivatives and subsequent increase in ionization efficiency. , Furthermore, the method is highly automated, which reduces possible human errors. It can be improved even more by converting the liquid chromatography method to ultraperformance liquid chromatography.…”

Gas Chromatographic Analysis of Histamine in Mahi-mahi ( Coryphaena hippurus )

“…It is known, however, that the GC-ECD is more sensitive than the GC-FID, particularly toward electronegative functional groups such as halogens (48). Hiemke et al (49) reported that the derivatized histamine is unstable in the presence of water and that up to 50% of the derivatives can be lost during preparation. Our observations showed that the histamine derivative was stable for short periods (2-3 days at refrigeration temperature), after which time smaller and broader peaks were seen in the chromatograms.…”

Distribution and Properties of Thiol S‐Methyltransferase in Rat Brain

“…A toluene-based scintillant (3 ml) was added, and the radioactivity measured by liquid scintillation counting. When cysteine or homocysteine was used as substrate, the amino groups were propionylated (Hiemke et al, 1978) prior to extraction. Methylated mercaptoethylamine was removed from the aqueous phase by liquid ion-exchange extraction.…”