Gas—liquid chromatography and mass spectrometry of aldononitrile acetates and partially methylated aldononitrile acetates

Dmitriev, Boris A.; Backinowsky, Leon V.; Chizhov, O. S.; Zolotarev, B. M.; Kochetkov, Nikolay K.

doi:10.1016/s0008-6215(00)86179-0

Cited by 94 publications

(21 citation statements)

References 3 publications

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“…Both fractions were incubated with galactose oxidase in 0.1 M phosphate buffer at pH 7.0 overnight at room temperature, The consumption of galactosamine from hexosamine fraction was detected using amino-acid analyzer with glucosamine as an internal standard. The fraction of neutral monosaccharide was freeze-dried after incubation with enzyme and analyzed by gasliquid chromatography in the form of aldononitrile acetates [9].…”

Section: Determination Of Monosaccharide Compositionmentioning

confidence: 99%

Somatic Antigen of Shigella dysenteriae Type 3. Structural Features of Specific Polysaccharide Chain

et al. 1975

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On mild acid hydrolysis of lipolysaccharide from Shigella dysenteriae, type 3 the O‐specific polysaccharide (hapten) was obtained which appeared to be acidic branched hexosaminoglycan. The repeating unit of this polysaccharide represents a pentasaccharide composed of two d‐galactose residues, N‐acetyl‐d‐galactosamine, d‐glucose and unidentified acidic component. On the basis of methylation analysis, periodate oxidation, partial acid hydrolysis and chromic anhydride oxidation it is concluded that the structure of the chemical repeating unit of polysaccharide is where Glcp, is glucopyranose, Galp is galactopyranose, Galf is galactofuranose, GalNAcp is 2‐acetamido‐2‐deoxygalactopyranose and where the configuration of galactofuranoside glycosidic linkage and the structure of the acidic monosaccharide A are not known.

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Section: Determination Of Monosaccharide Compositionmentioning

confidence: 99%

Somatic Antigen of Shigella dysenteriae Type 3. Structural Features of Specific Polysaccharide Chain

et al. 1975

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“…The filtrate was evaporated in vacuo and the residue was dried by repeated addition and distillation of toluene. To the residue were added hydroxyl- (11) glucose; (111) galactose; (IV) heptose amine hydrochloride (1 mg) and pyridine (0.5 ml) freshly distilled over P,O,. The mixture was heated in a stoppered flask at 100 "C for 30 min.…”

Section: Identification Of Neutrae Monosaccharidesmentioning

confidence: 99%

Immunochemical Studies on Shigella boydii Lipopolysaccharides

Dmitriev¹,

Backinowsky²,

Kochetkov³

et al. 1973

European Journal of Biochemistry

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An investigation of the sugar composition of lipopolysaccharides derived from fifteen welldefined Shigella boydii serotypes was carried out. All Sh. boydii lipopolysaccharides contained glucosarnine, glucose, galactose, heptose and 3-deoxyoctulosonic acid. These sugars were considered to represent the core polysaccharides and those except heptose and 3-deoxyoctulosonic acid could represent the constituents of the 0-specific polysaccharide chains. In addition to the basal sugars, the following monosaccharides were detected as constituents of the various Sh. boydii lipopolysaccharides : mannose, rhamnose, L-quinovosamine, galactosamine and 3,6-dideoxyhexose. As a result, a chemical classification of Sh. boydii lipopolysaccharides into eight chemotypes was achieved and this differs from the accepted Sh. boydii classification. Genus Shigella comprises the pathogenic organism which cause dysentery. This makes the systematic investigation on lipopolysaccharides of Shigella microorganisms of great significance for immunology as well as for taxonomy, biosynthesis, genetics and the evolutionary history of this important family of biopoly mers .34 Eur. J. Biochem., Vo1.34 MATERIALS AND METHODS Bacteria and LipopolysaccharidesThe strains of all fifteen serotypes of Sh. boydii used in this study were selected from the collection of the I. I. Mechnikov Institute of Vaccine and Sera.The strains of serotypes 1, 4, 7, 11 and 12 were isolated in the U.S.S.R., the strains of serotypes 2, 3, 6 and 8 came from the U.S.A., those of 5,9 and 10 were from Poland and strains 13, 14 and 15 from England. The bacterial cultures were tested for purity by slide agglutination tests with the adsorbed type sera and by the cultivation on appropriate media. The study showed all the microorganisms to be in S-form.To achieve more detailed serological characteristics of the bacterial cultures, the reciprocal antigenic relations inside the group of Sh. boydii were examined. The antisera were prepared by the immunisation of rabbits with preheated (100 "C) and formaldehyde (0.3O/,, v/v) pretreated microbial suspensions. The antigenic individuality of Sh. boydii serotypes was confirmed in cross agglutination tests with the exception of serotypes 10 and 11, which showed intensive cross reaction. There was no inagglutinability of cultures in the 0-sera. For the preparation of the microbial cells, broth cultures (4 h) were inoculated onto sightly alkaline agar media. Each strain was placed in 25 gels and incubated for 18 to 20 h at 37 "C. The gels were harvested by gentle shaking with saline containing 2O/, (w/v) phenol.The suspension was kept overnight at 37 "C, thereby

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“…2A and B). Their EI-MS spectra are of interest since, as expected, cleavage took place preferentially between vic-CHOMe groups [22]. [23], that of Nicotiana plumbaginifolia cell cultures [24] and that present in the fruit of Olea europaea [25].…”

Section: Resultsmentioning

confidence: 99%

New 4-O-substituted xylosyl units in the xyloglucan from leaves of Hymenaea courbaril

Busato

Vargas-Rechia

Gorin

et al. 2005

International Journal of Biological Macromolecules

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Gas—liquid chromatography and mass spectrometry of aldononitrile acetates and partially methylated aldononitrile acetates

Cited by 94 publications

References 3 publications

Somatic Antigen of Shigella dysenteriae Type 3. Structural Features of Specific Polysaccharide Chain

Somatic Antigen of Shigella dysenteriae Type 3. Structural Features of Specific Polysaccharide Chain

Immunochemical Studies on Shigella boydii Lipopolysaccharides

New 4-O-substituted xylosyl units in the xyloglucan from leaves of Hymenaea courbaril

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