Gas chromatographic resolution of substituted glutamic acid enantiomers

Synthesis and Pharmacological Characterization of Aminocyclopentanetricarboxylic Acids: New Tools to Discriminate between Metabotropic Glutamate Receptor Subtypes

“…Gas chromatography (GC) was performed on Varian 3700 or 3400 chromatographs equipped with a flame ionisation detector, using helium (1 bar) as carrier gas and fitted with a Flexibond OV-1701 capillary column (15 m × 0.25 mm, Pierce Chemical Co.) or a Chirasil-Val capillary column (50 m × 0.32 mm, Alltech). GC of amino acids was performed after derivatization to N -(trifluoroacetyl)- O -isopropyl esters …”

Synthesis and Pharmacological Characterization of Aminocyclopentanetricarboxylic Acids: New Tools to Discriminate between Metabotropic Glutamate Receptor Subtypes

“…Gas chromatography (GC) was performed on Varian 3700 or 3400 chromatographs equipped with a flame ionisation detector, using helium (1 bar) as carrier gas and fitted with a Flexibond OV-1701 capillary column (15 m × 0.25 mm, Pierce Chemical Co.) or a Chirasil-Val capillary column (50 m × 0.32 mm, Alltech). GC of amino acids was performed after derivatization to N -(trifluoroacetyl)- O -isopropyl esters …”

γ‐Fluorinated Analogues of Glutamic Acid and Glutamine

“…4-Fluoroglutamic acid 1 was esterified and injected directly into the injection port at the temperature range of 190 to 230°C allowing separation of erythro and threo isomers on several types of columns (Tolman et al, 1984). Soon after, Azerad reported gas chromatography resolution of the four stereoisomers as their N-trifluoroacetyl diisopropyl esters (Maurs et al, 1985).…”

Synthesis and polymerization of benzyl (3R,4R)-3-methylmalolactonate via enzymatic preparation of the chiral precursor

“…Samples (0.5-1 mg) were derivatized using a 1.5M solution of dry HCl in isopropanol for 30 min at 100°C. 19 After drying with nitrogen, the residue was dissolved in 200 µl of CH 2 The lactones were analyzed on a Varian 3300 chromatograph equipped with a fused-silica capillary column (Cydex-B, Scientific Glass Engineering (Austin, TX), 25 m × 0.33 mm id, d f = 0.25 µm). Nitrogen was used as the carrier gas, the column flow rate was 1.4 ml.min −1 .…”

“…Samples (0.5-1 mg) were derivatized using a 1.5M solution of dry HCl in isopropanol for 30 min at 100°C. 19 After drying with nitrogen, the residue was dissolved in 200 µl of CH 2 Cl 2 and 50 µl of trifluoroacetic anhydride and left for 30 min at room temperature. Excess reagent was evaporated under a stream of nitrogen and the residue dissolved in 0.5 ml of dichloromethane for injection.…”

Synthesis and Pharmacological Characterization of Aminocyclopentanetricarboxylic Acids: New Tools to Discriminate between Metabotropic Glutamate Receptor Subtypes

“…Gas chromatography (GC) was performed on Varian 3700 or 3400 chromatographs equipped with a flame ionisation detector, using helium (1 bar) as carrier gas and fitted with a Flexibond OV-1701 capillary column (15 m × 0.25 mm, Pierce Chemical Co.) or a Chirasil-Val capillary column (50 m × 0.32 mm, Alltech). GC of amino acids was performed after derivatization to N -(trifluoroacetyl)- O -isopropyl esters …”

γ‐Fluorinated Analogues of Glutamic Acid and Glutamine

“…4-Fluoroglutamic acid 1 was esterified and injected directly into the injection port at the temperature range of 190 to 230°C allowing separation of erythro and threo isomers on several types of columns (Tolman et al, 1984). Soon after, Azerad reported gas chromatography resolution of the four stereoisomers as their N-trifluoroacetyl diisopropyl esters (Maurs et al, 1985).…”

Synthesis and polymerization of benzyl (3R,4R)-3-methylmalolactonate via enzymatic preparation of the chiral precursor

“…Samples (0.5-1 mg) were derivatized using a 1.5M solution of dry HCl in isopropanol for 30 min at 100°C. 19 After drying with nitrogen, the residue was dissolved in 200 µl of CH 2 The lactones were analyzed on a Varian 3300 chromatograph equipped with a fused-silica capillary column (Cydex-B, Scientific Glass Engineering (Austin, TX), 25 m × 0.33 mm id, d f = 0.25 µm). Nitrogen was used as the carrier gas, the column flow rate was 1.4 ml.min −1 .…”

“…Samples (0.5-1 mg) were derivatized using a 1.5M solution of dry HCl in isopropanol for 30 min at 100°C. 19 After drying with nitrogen, the residue was dissolved in 200 µl of CH 2 Cl 2 and 50 µl of trifluoroacetic anhydride and left for 30 min at room temperature. Excess reagent was evaporated under a stream of nitrogen and the residue dissolved in 0.5 ml of dichloromethane for injection.…”

Synthesis and Pharmacological Characterization of Aminocyclopentanetricarboxylic Acids: New Tools to Discriminate between Metabotropic Glutamate Receptor Subtypes

“…Gas chromatography (GC) was performed on Varian 3700 or 3400 chromatographs equipped with a flame ionisation detector, using helium (1 bar) as carrier gas and fitted with a Flexibond OV-1701 capillary column (15 m × 0.25 mm, Pierce Chemical Co.) or a Chirasil-Val capillary column (50 m × 0.32 mm, Alltech). GC of amino acids was performed after derivatization to N -(trifluoroacetyl)- O -isopropyl esters …”

γ‐Fluorinated Analogues of Glutamic Acid and Glutamine

“…4-Fluoroglutamic acid 1 was esterified and injected directly into the injection port at the temperature range of 190 to 230°C allowing separation of erythro and threo isomers on several types of columns (Tolman et al, 1984). Soon after, Azerad reported gas chromatography resolution of the four stereoisomers as their N-trifluoroacetyl diisopropyl esters (Maurs et al, 1985).…”

Synthesis and polymerization of benzyl (3R,4R)-3-methylmalolactonate via enzymatic preparation of the chiral precursor

“…Samples (0.5-1 mg) were derivatized using a 1.5M solution of dry HCl in isopropanol for 30 min at 100°C. 19 After drying with nitrogen, the residue was dissolved in 200 µl of CH 2 The lactones were analyzed on a Varian 3300 chromatograph equipped with a fused-silica capillary column (Cydex-B, Scientific Glass Engineering (Austin, TX), 25 m × 0.33 mm id, d f = 0.25 µm). Nitrogen was used as the carrier gas, the column flow rate was 1.4 ml.min −1 .…”

“…Samples (0.5-1 mg) were derivatized using a 1.5M solution of dry HCl in isopropanol for 30 min at 100°C. 19 After drying with nitrogen, the residue was dissolved in 200 µl of CH 2 Cl 2 and 50 µl of trifluoroacetic anhydride and left for 30 min at room temperature. Excess reagent was evaporated under a stream of nitrogen and the residue dissolved in 0.5 ml of dichloromethane for injection.…”