Garcinia mangostana peel extracts exhibit hepatoprotective activity against thioacetamide-induced liver cirrhosis in rats

Abood, Walaa Najm; Bradosty, Sarwan W.; Shaikh, Faiyaz K.; Salehen, Nur'Ain; Farghadani, Reyhaneh; Agha, Nabaz Fisal Shakir; Al-Medhtiy, Morteta H.; Kamil, Treska Dhia Aldeen; Agha, Ali Salim; Abdulla, Mahmood Ameen

doi:10.1016/j.jff.2020.104200

Cited by 26 publications

(36 citation statements)

References 43 publications

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“…Elevated serum aminotransferase levels are a marker of liver injury, therefore we measured ALT and AST in mouse serum to assess the extent of liver injury. Interestingly, the results showed that TAA significantly increased serum ALT and AST concentrations, which was consistent with the results of some researchers (Tsai et al, 2021), while that DHM was able to modify this phenomenon (Salama et al, 2013;Abood et al, 2020;Elnfarawy et al, 2020). In addition, liver indices were measured in mice and the results represent the effect of DHM with hepatoprotective activity on the liver weight/body weight ratio, which was consistent with previous studies (Salama et al, 2013;Abood et al, 2020;Elnfarawy et al, 2020).…”

Section: Discussionsupporting

confidence: 91%

Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

et al. 2021

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As a natural active substance, dihydromyricetin (DHM) has been proven to have good hepatoprotective activity. However, the therapeutic effect of DHM on liver fibrosis, which has become a liver disease threatening the health of people around the world, has not been studied to date. The purpose of this study was to investigate the effect of DHM as a new nutritional supplement on thioacetamide (TAA)-induced liver fibrosis. The liver fibrosis model was established by intraperitoneal injection of TAA (200 mg/kg, every 3 days) for 8 weeks, and oral administration of DHM (20 mg/kg and 40 mg/kg, daily) after 4 weeks of TAA-induced liver fibrosis. The results showed that DHM treatment significantly inhibited the activities of alanine aminotransferase (ALT) (37.81 ± 7.62 U/L) and aspartate aminotransferase (AST) (55.18 ± 10.94 U/L) in serum of liver fibrosis mice, and increased the levels of superoxide dismutase (SOD) and glutathione (GSH) while reversed the level of malondialdehyde (MDA). In addition, histopathological examination illustrated that TAA induced the inflammatory infiltration, apoptosis and fibroatherosclerotic deposition in liver, which was further confirmed by western-blot and immunofluorescence staining. Moreover, DHM inhibited hepatocyte apoptosis by regulating the phosphorylation level of phosphatidylinositol 3-kinase (PI3K), protein kinase-B (AKT) and its downstream apoptotic protein family. Interestingly, immunofluorescence staining showed that DHM treatment significantly inhibited alpha smooth muscle actin (α-SMA), which was a marker of hepatic stellate cell activation, and regulated the expression of transforming growth factor (TGF-β1). Importantly, supplementation with DHM significantly inhibited the release of nuclear factor kappa-B (NF-κB) signaling pathway and pro-inflammatory factors in liver tissue induced by TAA, and improved liver fiber diseases, such as tumor necrosis factor alpha (TNF-α) and recombinant rat IL-1β (IL-1β). In conclusion, the evidence of this study revealed that DHM is a potential hepatoprotective and health factor, and which also provides the possibility for the treatment of liver fibrosis.

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Section: Discussionsupporting

confidence: 91%

Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

et al. 2021

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“…Recent studies indicates that α-MGS could attenuate lipopolysaccharide/d-galactosamine-induced liver pathological injury, and decrease the hepatic malondialdehyde level, serum ALT, AST, bilirubin and albumin. 21 , 22 The damage effect of γ-irradiation provokes oxidative stress in the liver tissue which is evidenced by the significant increase in the level of liver lipid peroxidation (MDA) concomitant with a significant decrease in the activity of the antioxidant enzymes SOD, CAT. The interaction of MDA and SOD demonstrated that SOD activities can indirectly reflect the scavenging capacity of oxygen free radicals.…”

Section: Discussionmentioning

confidence: 99%

Mangosteen Hinders Gamma Radiation-Mediated Oxidative Stress and Liver Injury by Down-Regulating TNF-α/NF-κB and Pro-Fibrotic Factor TGF-β1 Inducing Inflammatory Signaling

et al. 2021

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Background: Liver injury due to ionizing radiation exposure either accidental or after radiotherapy treatment, may lead to many alterations in proteins expression related to inflammation or apoptosis. Our study investigated the curative effect of Mangosteen (MGS) extract (fruit rind) against ionizing radiation (IR) induced liver damage. Methods: Hepatotoxicity was induced in Wister rats by exposure to an acute single dose (6 Gy) of IR while MGS was given orally to rats (500 mg/kg bwt) and administered daily for 30 days after irradiation. Results: MGS treatment has significantly attenuated redox imbalance state and toxicity induced by protracted exposure to gamma-rays in liver tissues, which was substantiated by the significant amelioration of liver function tests, MDA contents, antioxidant enzymes (SOD and CAT) activities and NO level. MGS inhibited also the inflammatory markers (TNF-alpha, IL-6 and CRP) and downregulated transcriptional factor NF-Kappa-B/TGF-β1. These alterations were concomitant with an improvement of the Proliferating cell nuclear antigen (PCNA) which is a protein expressed in the nuclei of cells during cell cycle and is important for both DNA synthesis and DNA repair. These results were confirmed by amelioration in histological and ultrastructural examinations. Conclusion: We concluded that MGS could ameliorate via minimizing significantly the amount of oxidative damage, inflammations disturbances and pro-apoptotic alternations induced by IR. MGS may be a promising supplement with protective effects from irradiation-induced injury such as TNF-α/NF-κB/TGF-β1 management.

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“…Adyab et al [6] observed that the supplementation of mangosteen flesh occurred from a concentration of 200 mg/kg and improved liver and kidney histopathological structures of rats almost completely to their normal structures after a highfat diet. Abood et al [12] stated that treatment with mangosteen peel's extract prevents liver cirrhosis and Copyright: Rusman, et al Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.…”

Section: Introductionmentioning

confidence: 99%

“…Abood et al . [ 12 ] stated that treatment with mangosteen peel’s extract prevents liver cirrhosis and maintains the parenchymal architecture of hepatocytes. However, there are no previous studies that confirm the effect of mangosteen peel infusion on the organs after H 2 O 2 induction.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

et al. 2021

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Background and Aim: Hydrogen peroxide (H2O2) is a free radical, widely use as a food preservative, may cause adverse effects to the body. Mangosteen contains various antioxidants may scavenge free radical produced by H2O2. This study examined the effects of mangosteen peel infusion on the liver and kidney of rats after H2O2 induction. Materials and Methods: Thirty-six female Wistar rats were divided into six groups: Negative control, positive control (1% H2O2), as well as mangosteen peel infusion groups at a concentration of 0.25; 0.5; 1, and 2%. H2O2 induction was administered orally every day for 2 months followed by mangosteen peel infusion treatment (1 month) after H2O2 induction for 1 month. At the end of the experiment, the rats were sacrificed. The liver and kidney of each rat were collected for reactive oxygen species (ROS) and histopathological examinations. Furthermore, blood was collected for creatinine examination. Results: H2O2 induction caused the highest ROS level elevation in the positive control group which was treated with H2O2 only. Meanwhile, 2% of mangosteen peel infusion returned the ROS levels to normal. It was also observed that mangosteen peel infusion restored creatinine levels to normal. Furthermore, 2% of mangosteen peel infusion improved the histopathological structure of the liver and kidneys after H2O2 induction. Conclusion: Mangosteen peel infusion especially at a concentration of 2% has the potential to improve liver and kidney structure and functions after H2O2 induction.

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Garcinia mangostana peel extracts exhibit hepatoprotective activity against thioacetamide-induced liver cirrhosis in rats

Cited by 26 publications

References 43 publications

Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

Mangosteen Hinders Gamma Radiation-Mediated Oxidative Stress and Liver Injury by Down-Regulating TNF-α/NF-κB and Pro-Fibrotic Factor TGF-β1 Inducing Inflammatory Signaling

Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

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