Gametophyte Morphology and Development of Six Species of Pteris (Pteridaceae) from Java Island Indonesia

Abstract

The morphology of sporophyte, the type of reproduction, and cytology of Pteris had been reported, while the gametophyte morphology of Pteris in Java island has not been studied yet. The objective of this study was to describe the gametophyte morphology and development of P. biaurita, P. ensiformis, P. exelsa, P. longipinnula, P. tripartita, and P. vittata in Java island. Spores were obtained from fertile leaves of Pteris plants originated from several locations in Java island. The number of spores per sporangium was counted from fresh fertile leaves with mature sporangia. As much as 0.002 g spores was sown in a transparent box with sterile medium contain of vermiculite, sphagnum moss, and perlite with ratio 2:2:1. The gametophyte development of each species was observed under a microscope every 7 days. The spores of P. ensiformis were germinated faster, ten days after sowing, while the spores of P. longipinnula were germinated slower, 18 days after sowing. The pattern of spore germination is Vittaria-type. The development of gametophyte is Ceratopteris-type in common, but in a few cases is the Adiantum-type. The gametophyte development of observed Pteris species is varied in six characters including the number of filament cell, germinated time, the formation time of notch and gametangia, margin shape, and development type.

Growth and Development of Apogamous Adiantum lunulatum Burm. f. Gametophyte from Dry and Humid Areas in Java Island

“…This result is in accordance to the previous study [19] that showed the gametophyte cells would continue to proliferate along with the age of the gametophyte and the total number of the proliferating cell was varied among species.…”

“…The medium was put in a plastic box, covered with a paper filter, and sterilized using boiling water and cool it down for one night, then as many as 0.003 g spores for each treatment were sown on media [19]. Growth and development of gametophytes were observed using stereo microscope once a week for and repeatedly under 10× for each treatment.…”

DESCRIPCIÓN DEL DESARROLLO MORFOGÉNICO DE LOS PRÓTALOS Y ESPORÓFITOS JOVENES DE Pteris quadriaurita RETZ. (PTERIDACEAE-POLYPODIIDAE) EN TRES SOPORTES NATURALES

“…Se han publicado varios estudios de la fase gametofítica del género de varias especies entre los que destacan las investigaciones de (Mehra, 1938) p. 202-210;(Crotty, 1967) p.105-117; (Cohen & Crotty, 1979) p. 179-182;(Laird & Sheffield, 1986) p. 139-143;(Gremmrich, 1988) p. 159-164; (Beri & Bir, 1993) p. 73-78;(Mendoza, Pérez-García, Reyes-Jaramillo, & Ricci, 1995) p. 51-57; (Huang, Chou, Hsieh, Wang, & Chiou, 2006) p. 261-268; (Letelier & Penailillo, 2007) p. 93-97; (Prada, Moreno, & Gabriel y Galan, 2008) p. 14-25; (Zhang, Shi, Zhang, & Jiang, 2008) p. 33-41; (Tanco, Martínez, & Bonomo, 2009) p. 10-17; (Martínez, 2010) p. 89-102; (Huang, Hsu, S-Y., Hsieh-Mei, & Chiou, 2011) p. 79-87;(Chao, Lui, Chiang, & Chiou, 2012) p. 1-7; (Martínez, Prada, Tanco, & Bonomo, 2013) p. 46-52; (Baskaran, Jeyachandran, & Meighias, 2014) p. 2350-2358; (Puspitasari, Chikmawati, & Praptosuwiryo, 2015) p. 98-104.…”

MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT <em>Davallia denticulata</em> dan <em>Davallia trichomanoides</em>

“…Pengamatan tipe reproduksi dilakukan untuk memperoleh sampel dengan tipe reproduksi seksual. Pengamatan dilakukan dengan penghitungan jumlah spora pada satu sporangium mengikuti Puspitasari et al (2015). Satu sporangium diletakkan di atas kaca benda yang telah ditetesi air, kemudian ditutup dengan kaca penutup.…”

“…Media tumbuh yang disiapkan, terdiri dari vermiculite, gambut, dan perlite (Puspitasari et al 2015). Sebanyak 3 komposisi perlakuan media diuji, yaitu media pertama (M1) terdiri dari vermiculite:lumut sphagnum:perlite, dengan perbandingan 2:2:1; media kedua (M2) terdiri dari vermiculite:perlite, dengan perbandingan 2:1; media ketiga (M3) terdiri dari vermiculite:lumut sphagnum, dengan perbandingan 1:1.…”