Galardin Inhibits Collagen Degradation by Rabbit Keratocytes by Inhibiting the Activation of Pro-Matrix Metalloproteinases

Enhancement by neutrophils of collagen degradation by corneal fibroblasts

“…We first examined collagen degradation in three-dimensional cultures of corneal fibroblasts or neutrophils. Consistent with our previous observations [6], corneal fibroblasts mediated collagen degradation in a time-dependent manner (Fig. 2A).…”

Enhancement by neutrophils of collagen degradation by corneal fibroblasts

“…We first examined collagen degradation in three-dimensional cultures of corneal fibroblasts or neutrophils. Consistent with our previous observations [6], corneal fibroblasts mediated collagen degradation in a time-dependent manner (Fig. 2A).…”

Dexamethasone Inhibition of IL-1–Induced Collagen Degradation by Corneal Fibroblasts in Three-Dimensional Culture

“…The cells were incubated in collagen gels for 48 hours in the presence of plasminogen, in the absence or presence of IL-1␤ (0.1 ng/mL), and in the presence of various concentrations of dexamethasone (0.01 to 1.0 nM). Consistent with our previous observations, 6 IL-1␤ markedly increased the extent of collagen degradation by corneal fibroblasts. Dexamethasone inhibited in a dose-dependent manner the collagen degradation by corneal fibroblasts apparent in the absence or presence of IL-1␤, with IL-1␤-induced collagen degradation being almost completely inhibited at a dexamethasone concentration of 1 nM (Fig.…”

Inhibition by Triptolide of IL-1–Induced Collagen Degradation by Corneal Fibroblasts

“…The cells were cultured in the presence of various concentrations of IL-1␤ for 48 hours. Consistent with our previous observations, 6 IL-1␤ increased collagen degradation by corneal fibroblasts in a dose-dependent manner. Its effect was statistically significant at a concentration of 0.001 ng/mL, and maximum effect was obtained at 0.1 ng/mL (Fig.…”

“…We also have shown that galardin inhibits collagen degradation by corneal fibroblasts by preventing the conversion of proMMPs to active MMPs. 6 Our present results, however, show that triptolide did not affect the plasmin activity in culture supernatants or the conversion of proMMPs to MMPs, but rather inhibited the synthesis of these proteases.…”

Enhancement by neutrophils of collagen degradation by corneal fibroblasts

“…We first examined collagen degradation in three-dimensional cultures of corneal fibroblasts or neutrophils. Consistent with our previous observations [6], corneal fibroblasts mediated collagen degradation in a time-dependent manner (Fig. 2A).…”

Dexamethasone Inhibition of IL-1–Induced Collagen Degradation by Corneal Fibroblasts in Three-Dimensional Culture

“…The cells were incubated in collagen gels for 48 hours in the presence of plasminogen, in the absence or presence of IL-1␤ (0.1 ng/mL), and in the presence of various concentrations of dexamethasone (0.01 to 1.0 nM). Consistent with our previous observations, 6 IL-1␤ markedly increased the extent of collagen degradation by corneal fibroblasts. Dexamethasone inhibited in a dose-dependent manner the collagen degradation by corneal fibroblasts apparent in the absence or presence of IL-1␤, with IL-1␤-induced collagen degradation being almost completely inhibited at a dexamethasone concentration of 1 nM (Fig.…”

Inhibition by Triptolide of IL-1–Induced Collagen Degradation by Corneal Fibroblasts

“…The cells were cultured in the presence of various concentrations of IL-1␤ for 48 hours. Consistent with our previous observations, 6 IL-1␤ increased collagen degradation by corneal fibroblasts in a dose-dependent manner. Its effect was statistically significant at a concentration of 0.001 ng/mL, and maximum effect was obtained at 0.1 ng/mL (Fig.…”

“…We also have shown that galardin inhibits collagen degradation by corneal fibroblasts by preventing the conversion of proMMPs to active MMPs. 6 Our present results, however, show that triptolide did not affect the plasmin activity in culture supernatants or the conversion of proMMPs to MMPs, but rather inhibited the synthesis of these proteases.…”

Enhancement by neutrophils of collagen degradation by corneal fibroblasts

“…We first examined collagen degradation in three-dimensional cultures of corneal fibroblasts or neutrophils. Consistent with our previous observations [6], corneal fibroblasts mediated collagen degradation in a time-dependent manner (Fig. 2A).…”

Dexamethasone Inhibition of IL-1–Induced Collagen Degradation by Corneal Fibroblasts in Three-Dimensional Culture

“…The cells were incubated in collagen gels for 48 hours in the presence of plasminogen, in the absence or presence of IL-1␤ (0.1 ng/mL), and in the presence of various concentrations of dexamethasone (0.01 to 1.0 nM). Consistent with our previous observations, 6 IL-1␤ markedly increased the extent of collagen degradation by corneal fibroblasts. Dexamethasone inhibited in a dose-dependent manner the collagen degradation by corneal fibroblasts apparent in the absence or presence of IL-1␤, with IL-1␤-induced collagen degradation being almost completely inhibited at a dexamethasone concentration of 1 nM (Fig.…”

Inhibition by Triptolide of IL-1–Induced Collagen Degradation by Corneal Fibroblasts

“…The cells were cultured in the presence of various concentrations of IL-1␤ for 48 hours. Consistent with our previous observations, 6 IL-1␤ increased collagen degradation by corneal fibroblasts in a dose-dependent manner. Its effect was statistically significant at a concentration of 0.001 ng/mL, and maximum effect was obtained at 0.1 ng/mL (Fig.…”

“…We also have shown that galardin inhibits collagen degradation by corneal fibroblasts by preventing the conversion of proMMPs to active MMPs. 6 Our present results, however, show that triptolide did not affect the plasmin activity in culture supernatants or the conversion of proMMPs to MMPs, but rather inhibited the synthesis of these proteases.…”